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Title: Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge

Abstract

This paper presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reduction in amine functionalities and phosphate groups. Near edge x-ray absorption fine structure(NEXAFS) measurements confirm the presence of additional oxide bonds upon DBD treatment, suggesting oxidation of the outer layer of the cell wall. Electron microscopy images show that the bacteria undergo physical distortion to varying degrees, resulting in deformation of the bacterial structure. The electromagnetic field around the DBD coil causes severe damage to the cell structure, possibly resulting in leakage of vital cellular materials. The oxidation and chemical modification of the bacterial components are evident from the Fourier transform infrared spectroscopy and NEXAFS results. The bacterial reculture experiments confirm inactivation of airborne E. coli upon treating with DBD.

Authors:
 [1];  [2];  [3];  [3];  [3];  [4];  [5];  [5];  [1];  [1];  [1];  [1];  [6]
  1. NASA Ames Research Center, Moffett Field, CA (United States)
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  3. Novaerus, Inc., Chicago, IL (United States)
  4. Aalto Univ., Espoo (Finland)
  5. Dublin City Univ., Dublin (Ireland)
  6. NASA Ames Research Center, Moffett Field, CA (United States); Univ. Space Research Association, Mountain View, CA (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1240000
Grant/Contract Number:  
AC02-76SF00515
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biointerphases
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1934-8630
Publisher:
AIP
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacteria; X-ray absorption spectroscopy; X-ray absorption near edge structure; carbon; Fourier transform infrared spectroscopy

Citation Formats

Romero-Mangado, Jaione, Nordlund, Dennis, Soberon, Felipe, Deane, Graham, Maughan, Kevin, Sainio, Sami, Singh, Gurusharan, Daniels, Stephen, Saunders, Ian T., Loftus, David, Meyyappan, M., Koehne, Jessica, and Gandhiraman, Ram P. Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge. United States: N. p., 2016. Web. doi:10.1116/1.4941367.
Romero-Mangado, Jaione, Nordlund, Dennis, Soberon, Felipe, Deane, Graham, Maughan, Kevin, Sainio, Sami, Singh, Gurusharan, Daniels, Stephen, Saunders, Ian T., Loftus, David, Meyyappan, M., Koehne, Jessica, & Gandhiraman, Ram P. Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge. United States. https://doi.org/10.1116/1.4941367
Romero-Mangado, Jaione, Nordlund, Dennis, Soberon, Felipe, Deane, Graham, Maughan, Kevin, Sainio, Sami, Singh, Gurusharan, Daniels, Stephen, Saunders, Ian T., Loftus, David, Meyyappan, M., Koehne, Jessica, and Gandhiraman, Ram P. 2016. "Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge". United States. https://doi.org/10.1116/1.4941367. https://www.osti.gov/servlets/purl/1240000.
@article{osti_1240000,
title = {Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge},
author = {Romero-Mangado, Jaione and Nordlund, Dennis and Soberon, Felipe and Deane, Graham and Maughan, Kevin and Sainio, Sami and Singh, Gurusharan and Daniels, Stephen and Saunders, Ian T. and Loftus, David and Meyyappan, M. and Koehne, Jessica and Gandhiraman, Ram P.},
abstractNote = {This paper presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reduction in amine functionalities and phosphate groups. Near edge x-ray absorption fine structure(NEXAFS) measurements confirm the presence of additional oxide bonds upon DBD treatment, suggesting oxidation of the outer layer of the cell wall. Electron microscopy images show that the bacteria undergo physical distortion to varying degrees, resulting in deformation of the bacterial structure. The electromagnetic field around the DBD coil causes severe damage to the cell structure, possibly resulting in leakage of vital cellular materials. The oxidation and chemical modification of the bacterial components are evident from the Fourier transform infrared spectroscopy and NEXAFS results. The bacterial reculture experiments confirm inactivation of airborne E. coli upon treating with DBD.},
doi = {10.1116/1.4941367},
url = {https://www.osti.gov/biblio/1240000}, journal = {Biointerphases},
issn = {1934-8630},
number = 1,
volume = 11,
place = {United States},
year = {Fri Feb 12 00:00:00 EST 2016},
month = {Fri Feb 12 00:00:00 EST 2016}
}

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Works referencing / citing this record:

Atmospheric Pressure Pulsed Plasma Induces Cell Death in Photosynthetic Organs via Intracellularly Generated ROS
journal, April 2017