Metaproteomics reveals functional shifts in microbial and human proteins during a preterm infant gut colonization case

Young, Jacque C.; Pan, Chongle; Adams, Rachel M.; Brooks, Brandon; Banfield, Jillian F.; Morowitz, Michael J.; Hettich, Robert L.

doi:10.1002/pmic.201400563

Title: Metaproteomics reveals functional shifts in microbial and human proteins during a preterm infant gut colonization case

Journal Article · Tue Jul 21 00:00:00 EDT 2015 · Proteomics

DOI:https://doi.org/10.1002/pmic.201400563· OSTI ID:1581112

Young, Jacque C. ^[1]; Pan, Chongle ^[2]; Adams, Rachel M. ^[1]; Brooks, Brandon ^[3]; Banfield, Jillian F. ^[3]; Morowitz, Michael J. ^[4]; Hettich, Robert L. ^[2]

Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of California, Berkeley, CA (United States)
Univ. of Pittsburgh, PA (United States)

The microbial colonization of the human gastrointestinal tract plays an important role in establishing health and homeostasis. However, the time-dependent functional signatures of microbial and human proteins during early colonization of the gut have yet to be determined. Thus, we employed shotgun proteomics to simultaneously monitor microbial and human proteins in fecal samples from a preterm infant during the first month of life. Microbial community complexity and functions increased over time, with compositional changes that were consistent with previous metagenomic and rRNA gene data indicating three distinct colonization phases. Overall microbial community functions were established relatively early in development and remained stable. Human proteins detected included those responsible for epithelial barrier function and antimicrobial activity. Some neutrophil-derived proteins increased in abundance early in the study period, suggesting activation of the innate immune system. Moreover, abundances of cytoskeletal and mucin proteins increased later in the time course, suggestive of subsequent adjustment to the increased microbial load. Our study provides the first snapshot of coordinated human and microbial protein expression in the infant gut during early development.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH); March of Dimes Foundation; National Science Foundation (NSF)

Grant/Contract Number:: AC02-05CH11231; 5-FY10-103; AC05-00OR22725

OSTI ID:: 1581112

Alternate ID(s):: OSTI ID: 1237608

Journal Information:: Proteomics, Vol. 15, Issue 20; ISSN 1615-9853

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 39 works

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