Insights into the effect of dilute acid, hot water or alkaline pretreatment on cellulose accessible surface area and the overall porosity of Populus

Meng, Xianzhi; Wells, Tyrone; Sun, Qining; Huang, Fang; Ragauskas, Arthur J.

doi:10.1039/C5GC00689A

Title: Insights into the effect of dilute acid, hot water or alkaline pretreatment on cellulose accessible surface area and the overall porosity of Populus

Journal Article · Fri Jun 19 00:00:00 EDT 2015 · Green Chemistry

DOI:https://doi.org/10.1039/C5GC00689A· OSTI ID:1237144

Meng, Xianzhi ^[1]; Wells, Tyrone ^[2]; Sun, Qining ^[2]; Huang, Fang ^[2]; Ragauskas, Arthur J. ^[3]

Georgia Inst. of Technology, Atlanta, GA (United States). Bioenergy Science Center
Georgia Inst. of Technology, Atlanta, GA (United States). Renewable Bioproducts Institute
Univ. of Tennessee, Knoxville, TN (United States). Bioenergy Science Center, Dept. of Chemical and Biomolecular Engineering, and Dept. of Forestry, Wildlife, and Fisheries

Pretreatment is known to render biomass more reactive to cellulase by altering the chemical compositions as well as physical structures of biomass. Simons stain technique along with mercury porosimetry were applied on the acid, neutral, and alkaline pretreated materials to measure the accessible surface area of cellulose and pore size distribution of Populus. Results indicated that acid pretreatment is much more effective than water and alkaline pretreatment in terms of cellulose accessibility increase. Further investigation suggests that lignin does not dictate cellulose accessibility to the extent that hemicellulose does, but it does restrict xylan accessibility which in turn controls the access of cellulase to cellulose. The most interesting finding is that severe acid pretreatment significantly decreases the average pore size, i.e., 90% average size decrease could be observed after 60 min dilute acid pretreatment at 160 °C; moreover, the nano-pore space formed between coated microfibrils is increased after pretreatment, especially for the acid pretreatment, suggesting this particular type of biomass porosity is probably the most fundamental barrier to effective enzymatic hydrolysis.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1237144

Journal Information:: Green Chemistry, Vol. 17; ISSN 1463-9262

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 132 works

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Title: Insights into the effect of dilute acid, hot water or alkaline pretreatment on cellulose accessible surface area and the overall porosity of Populus

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