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Title: Keto-isovalerate decarboxylase enzymes and methods of use thereof

Patent ·
OSTI ID:1236558

Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.

Research Organization:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AR0000006
Assignee:
Butamax Advanced Biofuels LLC
Patent Number(s):
9,238,828
Application Number:
13/560,147
OSTI ID:
1236558
Resource Relation:
Patent File Date: 2012 Jul 27
Country of Publication:
United States
Language:
English

References (57)

Use of the valine biosynthetic pathway to convert glucose into isobutanol journal December 2010
Biochemical and molecular characterization of α-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis journal September 2004
Branched-Chain Keto Acid Decarboxylase from Lactococcus lactis (KdcA), a Valuable Thiamine Diphosphate-Dependent Enzyme for Asymmetric C-C Bond Formation journal June 2007
Application of α-keto acid decarboxylases in biotransformations journal June 1998
Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes journal July 2009
Aspartate-27 and glutamate-473 are involved in catalysis by Zymomonas mobilis pyruvate decarboxylase journal April 1999
The role of induced fit and conformational changes of enzymes in specificity and catalysis journal March 1988
Identification, Cloning, and Characterization of a Lactococcus lactis Branched-Chain  -Keto Acid Decarboxylase Involved in Flavor Formation journal January 2005
Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase journal December 2006
Solvent tolerant microorganisms and methods of isolation patent June 2009
Fermentive production of four carbon alcohols patent December 2010
Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes patent March 2011
Fermentive production of four carbon alcohols patent August 2011
Solvent tolerant microorganisms patent September 2011
Ketol-acid reductoisomerase using NADH patent March 2012
Fermentive production of four carbon alcohols patent May 2012
Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans patent May 2012
Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase patent June 2012
Ketol-acid reductoisomerase using NADH patent July 2012
Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof patent August 2012
Fermentive production of four carbon alcohols patent September 2012
Fermentive production of four carbon alcohols patent October 2012
Production of four carbon alcohols using improved strain patent February 2013
Yeast strain for production of four carbon alcohols patent March 2013
Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria patent June 2013
Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway patent June 2013
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis patent June 2013
Strain comprising increased expression of a CFA coding region for butanol production patent August 2013
Yeast with increased butanol tolerance involving filamentous growth response patent October 2013
Yeast with increased butanol tolerance involving a multidrug efflux pump gene patent December 2013
Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria patent January 2014
Expression of hexose kinase in recombinant host cells patent January 2014
Strain for butanol production with increased membrane unsaturated trans fatty acids patent February 2014
Enhanced pyruvate to acetolactate conversion in yeast patent March 2014
Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans patent April 2014
Fermentive production of four carbon alcohols patent May 2014
Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols patent July 2014
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis patent July 2014
Yeast with increased butanol tolerance involving cell wall integrity pathway patent August 2014
Production of isobutanol in yeast mitochondria patent September 2014
Fermentive production of four carbon alcohols patent September 2014
Fermentive Production Of Four Carbon Alcohols patent-application July 2008
Identification and use of Bacterial [2FE-2S] Dihydroxy-acid Dehydratases patent-application April 2010
Enhanced Iron-sulfur Cluster Formation for Increased Dihydroxy-Acid Dehydratase Activity in Lactic Acid Bacteria patent-application April 2010
Carbon Pathway Optimized Production Hosts for the Production of Isobutanol patent-application May 2010
Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol patent-application March 2011
Bacterial Strains for Butanol Production patent-application August 2011
Fermentive Production of Isobutanol Using Highly Effective Ketol-acid Reductoisomerase Enzymes patent-application October 2011
Engineering Resistance to Aliphatic Alcohols patent-application March 2012
Recombinant host cells comprising phosphoketolases patent-application June 2012
Fermentive Production of Four Carbon Alcohols patent-application August 2012
Lignocellulosic Hydrolysates as Feedstocks for Isobutanol Fermentation patent-application February 2013
Acetate Supplementation of Medium for Butanologens patent-application September 2013
Host Cells and Methods for Production of Isobutanol patent-application January 2014
Host Cells and Methods for Production of Isobutanol patent-application January 2014
Activity of FE-S Cluster Requiring Proteins patent-application February 2014
Dhad Variants for Butanol Production patent-application July 2014