Exploiting algal NADPH oxidase for biophotovoltaic energy

Anderson, Alexander; Laohavisit, Anuphon; Blaby, Ian K.; Bombelli, Paolo; Howe, Christopher J.; Merchant, Sabeeha S.; Davies, Julia M.; Smith, Alison G.

doi:10.1111/pbi.12332

Title: Exploiting algal NADPH oxidase for biophotovoltaic energy

Journal Article · Thu Jan 29 00:00:00 EST 2015 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12332· OSTI ID:1234170

Anderson, Alexander ^[1]; Laohavisit, Anuphon ^[1]; Blaby, Ian K. ^[2]; Bombelli, Paolo ^[3]; Howe, Christopher J. ^[3]; Merchant, Sabeeha S. ^[4]; Davies, Julia M. ^[1]; Smith, Alison G. ^[1]

Department of Plant Sciences University of Cambridge Cambridge UK
Department of Chemistry and Biochemistry UCLA Los Angeles CA USA
Department of Biochemistry University of Cambridge Cambridge UK
Institute for Genomics and Proteomics UCLA Los Angeles CA USA

Photosynthetic microbes exhibit light-dependent electron export across the cell membrane, which can generate electricity in biological photovoltaic (BPV) devices. How electrons are exported remains to be determined; the identification of mechanisms would help selection or generation of photosynthetic microbes capable of enhanced electrical output. We show that plasma membrane NADPH oxidase activity is a significant component of light-dependent generation of electricity by the unicellular green alga Chlamydomonas reinhardtii. NADPH oxidases export electrons across the plasma membrane to form superoxide anion from oxygen. The C. reinhardtii mutant lacking the NADPH oxidase encoded by RBO1 is impaired in both extracellular superoxide anion production and current generation in a BPV device. Complementation with the wild-type gene restores both capacities, demonstrating the role of the enzyme in electron export. Monitoring light-dependent extracellular superoxide production with a colorimetric assay is shown to be an effective way of screening for electrogenic potential of candidate algal strains. The results show that algal NADPH oxidases are important for superoxide anion production and open avenues for optimizing the biological component of these devices.

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Research Organization:: Univ. of Cambridge, Cambridge (United Kingdom); Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: DE–FC02–02ER63421; FC02-02ER63421

OSTI ID:: 1234170

Alternate ID(s):: OSTI ID: 1234171; OSTI ID: 1363910; OSTI ID: 1438189

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Vol. 14 Journal Issue: 1; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 28 works

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