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Title: Genome-scale metabolic reconstructions and theoretical investigation of methane conversion in Methylomicrobium buryatense strain 5G(B1)

Journal Article · · Microbial Cell Factories
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Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accurate metabolic model coupled with flux balance analysis can provide a solid interpretative framework for experimental data analyses and integration.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Grant/Contract Number:
AR0000350; AC36-08GO28308
OSTI ID:
1618797
Alternate ID(s):
OSTI ID: 1233694
Report Number(s):
NREL/JA-5100-65604; 188; PII: 377
Journal Information:
Microbial Cell Factories, Journal Name: Microbial Cell Factories Vol. 14 Journal Issue: 1; ISSN 1475-2859
Publisher:
Springer Science + Business MediaCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 84 works
Citation information provided by
Web of Science

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Cited By (18)

A tale of two methane monooxygenases journal November 2016
Methane utilization in Methylomicrobium alcaliphilum 20ZR: a systems approach journal February 2018
Reconstruction of a Genome Scale Metabolic Model of the polyhydroxybutyrate producing methanotroph Methylocystis parvus OBBP journal June 2019
Biological conversion of methane to putrescine using genome-scale model-guided metabolic engineering of a methanotrophic bacterium Methylomicrobium alcaliphilum 20Z journal June 2019
Metagenomic Binning Recovers a Transcriptionally Active Gammaproteobacterium Linking Methanotrophy to Partial Denitrification in an Anoxic Oxygen Minimum Zone journal February 2017
Rare Earth Elements Alter Redox Balance in Methylomicrobium alcaliphilum 20ZR journal November 2018
A Genome-Scale Metabolic Model for Methylococcus capsulatus (Bath) Suggests Reduced Efficiency Electron Transfer to the Particulate Methane Monooxygenase journal December 2018
Metabolic engineering of methanotrophs and its application to production of chemicals and biofuels from methane: Biofuel energy consumption-Economic growth relationship: An empirical investigation of Brazil journal August 2016
A modular approach for high-flux lactic acid production from methane in an industrial medium using engineered Methylomicrobium buryatense 5GB1 journal April 2018
Reconstruction of methanol and formate metabolic pathway in non-native host for biosynthesis of chemicals and biofuels journal August 2016
Muconic acid production from methane using rationally-engineered methanotrophic biocatalysts journal January 2019
Industrial biomanufacturing: The future of chemical production journal January 2017
Genomic Evidence for Simultaneous Optimization of Transcription and Translation through Codon Variants in the pmoCAB Operon of Type Ia Methanotrophs journal July 2019
A comparative transcriptome analysis of the novel obligate methanotroph Methylomonas sp. DH-1 reveals key differences in transcriptional responses in C1 and secondary metabolite pathways during growth on methane and methanol journal February 2019
Role of the malic enzyme in metabolism of the halotolerant methanotroph Methylotuvimicrobium alcaliphilum 20Z journal November 2019
Fatty Acid Biosynthesis Pathways in Methylomicrobium buryatense 5G(B1) journal January 2017
Oxygen-limited metabolism in the methanotroph Methylomicrobium buryatense 5GB1C journal January 2017
Genome-scale metabolic reconstruction and metabolic versatility of an obligate methanotroph Methylococcus capsulatus str. Bath journal January 2019

Linked Research (7)

Figures / Tables (7)

Fig. 1(p. 3)figure Fig. 1
Fig. 2(p. 4)figure Fig. 2
Table 1(p. 6)table Table 1
Table 1 (Continued)(p. 7)table Table 1 (Continued)
Table 1 (Continued)(p. 8)table Table 1 (Continued)
Fig. 3(p. 10)figure Fig. 3
Table 2(p. 11)table Table 2

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Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
methane metabolism
flux balance model
Methylomicrobium buryatense
strains 5G and 5G(B1)
metabolic engineering of methane utilization