This software was developed to measure absolute fluorescent intensities of gel pads on a microchip in units defined by a standard fluorescent slide. It can accomodate varying measurement conditions (e.g. exposure time, sensitivity of detector, resolution of detector, etc.) as well as fluorescent microscopes with non-uniform sensitivity across their field of view allowing the user to compare measurements done on different detectors with varying exposure times, sensitivities, and resolutions. The software is designed both to operate Roper Scientific, Inc. cameras and to use image files produced by the program supplied with that equipment for its calculations. the intensity of the gel pad signal is computed so as to reduce background influence.
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@misc{osti_1230614,
title = {Biochip Image Grid Normalization Absolute Signal Fluorescence Measurement Using, Version 00},
author = {Alferov, Oleg},
abstractNote = {This software was developed to measure absolute fluorescent intensities of gel pads on a microchip in units defined by a standard fluorescent slide. It can accomodate varying measurement conditions (e.g. exposure time, sensitivity of detector, resolution of detector, etc.) as well as fluorescent microscopes with non-uniform sensitivity across their field of view allowing the user to compare measurements done on different detectors with varying exposure times, sensitivities, and resolutions. The software is designed both to operate Roper Scientific, Inc. cameras and to use image files produced by the program supplied with that equipment for its calculations. the intensity of the gel pad signal is computed so as to reduce background influence.},
doi = {},
url = {https://www.osti.gov/biblio/1230614},
year = {Tue Apr 17 00:00:00 EDT 2001},
month = {Tue Apr 17 00:00:00 EDT 2001},
note =
}
