The procedure solves the problem of calling the DNA base pair sequence from two channel electropherogram separations in an automated fashion. The core of the program involves a peak picking algorithm based upon first, second, and third derivative spectra for each electropherogram channel, signal levels as a function of time, peak spacing, base pair signal to noise sequence patterns, frequency vs ratio of the two channel histograms, and confidence levels generated during the run. The ratios of the two channels at peak centers can be used to accurately and reproducibly determine the base pair sequence. A further enhancement is a novel Gaussian deconvolution used to determine the peak heights used in generating the ratio.
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Yeung, Edward S., Hall, David R., and Hazen, Kevin H. Automated DNA Base Pair Calling Algorithm.
Computer software. Vers. 00. DOE/DP. 7 Jul. 1999.
Web.
@misc{osti_1230482,
title = {Automated DNA Base Pair Calling Algorithm, Version 00},
author = {Yeung, Edward S. and Hall, David R. and Hazen, Kevin H.},
abstractNote = {The procedure solves the problem of calling the DNA base pair sequence from two channel electropherogram separations in an automated fashion. The core of the program involves a peak picking algorithm based upon first, second, and third derivative spectra for each electropherogram channel, signal levels as a function of time, peak spacing, base pair signal to noise sequence patterns, frequency vs ratio of the two channel histograms, and confidence levels generated during the run. The ratios of the two channels at peak centers can be used to accurately and reproducibly determine the base pair sequence. A further enhancement is a novel Gaussian deconvolution used to determine the peak heights used in generating the ratio.},
doi = {},
url = {https://www.osti.gov/biblio/1230482},
year = {Wed Jul 07 00:00:00 EDT 1999},
month = {Wed Jul 07 00:00:00 EDT 1999},
note =
}
