Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production

Park, Sang‐Hyuck; Ong, Rebecca Garlock; Sticklen, Mariam

doi:10.1111/pbi.12505

Title: Strategies for the production of cell wall‐deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production

Journal Article · Wed Dec 02 00:00:00 EST 2015 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12505· OSTI ID:1227588

Park, Sang‐Hyuck ^[1];

^[2]; Sticklen, Mariam ^[1]

Department of Plant, Soil and Microbial Sciences Michigan State University East Lansing MI USA
Department of Chemical Engineering and Materials Science DOE Great Lakes Bioenergy Research Center Michigan State University Lansing MI USA, Department of Chemical Engineering Michigan Technological University Houghton MI USA

Summary Microbial cell wall‐deconstructing enzymes are widely used in the food, wine, pulp and paper, textile, and detergent industries and will be heavily utilized by cellulosic biorefineries in the production of fuels and chemicals. Due to their ability to use freely available solar energy, genetically engineered bioenergy crops provide an attractive alternative to microbial bioreactors for the production of cell wall‐deconstructing enzymes. This review article summarizes the efforts made within the last decade on the production of cell wall‐deconstructing enzymes in planta for use in the deconstruction of lignocellulosic biomass. A number of strategies have been employed to increase enzyme yields and limit negative impacts on plant growth and development including targeting heterologous enzymes into specific subcellular compartments using signal peptides, using tissue‐specific or inducible promoters to limit the expression of enzymes to certain portions of the plant or certain times, and fusion of amplification sequences upstream of the coding region to enhance expression. We also summarize methods that have been used to access and maintain activity of plant‐generated enzymes when used in conjunction with thermochemical pretreatments for the production of lignocellulosic biofuels.

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Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1227588

Alternate ID(s):: OSTI ID: 1227591; OSTI ID: 1363935

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Vol. 14 Journal Issue: 6; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 25 works

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