skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer

Abstract

A significant limiting factor in achieving high spatial resolution for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is the size of the laser spot at the sample surface. We present modifications to the beam-delivery optics of a commercial MALDI-linear ion trap-Orbitrap instrument, incorporating an external Nd:YAG laser, beam-shaping optics, and an aspheric focusing lens, to reduce the minimum laser spot size from ~50 μm for the commercial configuration down to ~9 μm for the modified configuration. This improved system was applied for MALDI-MS imaging of cross sections of juvenile maize leaves at 5-μm spatial resolution using an oversampling method. There are a variety of different metabolites including amino acids, glycerolipids, and defense-related compounds were imaged at a spatial resolution well below the size of a single cell. Such images provide unprecedented insights into the metabolism associated with the different tissue types of the maize leaf, which is known to asymmetrically distribute the reactions of C4 photosynthesis among the mesophyll and bundle sheath cell types. The metabolite ion images correlate with the optical images that reveal the structures of the different tissues, and previously known and newly revealed asymmetric metabolic features are observed.

Authors:
 [1];  [2];  [1];  [1]
  1. Iowa State Univ., Ames, IA (United States); Ames Lab., Ames, IA (United States)
  2. Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1226917
Report Number(s):
IS-J 8571
Journal ID: ISSN 1618-2642; PII: 8460
Grant/Contract Number:  
AC02-07CH11358
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Analytical and Bioanalytical Chemistry
Additional Journal Information:
Journal Volume: 407; Journal Issue: 8; Journal ID: ISSN 1618-2642
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; mass spectrometry imaging; metabolite; maize

Citation Formats

Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., and Lee, Young Jin. Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer. United States: N. p., 2015. Web. doi:10.1007/s00216-015-8460-5.
Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., & Lee, Young Jin. Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer. United States. https://doi.org/10.1007/s00216-015-8460-5
Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., and Lee, Young Jin. 2015. "Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer". United States. https://doi.org/10.1007/s00216-015-8460-5. https://www.osti.gov/servlets/purl/1226917.
@article{osti_1226917,
title = {Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer},
author = {Korte, Andrew R. and Yandeau-Nelson, Marna D. and Nikolau, Basil J. and Lee, Young Jin},
abstractNote = {A significant limiting factor in achieving high spatial resolution for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is the size of the laser spot at the sample surface. We present modifications to the beam-delivery optics of a commercial MALDI-linear ion trap-Orbitrap instrument, incorporating an external Nd:YAG laser, beam-shaping optics, and an aspheric focusing lens, to reduce the minimum laser spot size from ~50 μm for the commercial configuration down to ~9 μm for the modified configuration. This improved system was applied for MALDI-MS imaging of cross sections of juvenile maize leaves at 5-μm spatial resolution using an oversampling method. There are a variety of different metabolites including amino acids, glycerolipids, and defense-related compounds were imaged at a spatial resolution well below the size of a single cell. Such images provide unprecedented insights into the metabolism associated with the different tissue types of the maize leaf, which is known to asymmetrically distribute the reactions of C4 photosynthesis among the mesophyll and bundle sheath cell types. The metabolite ion images correlate with the optical images that reveal the structures of the different tissues, and previously known and newly revealed asymmetric metabolic features are observed.},
doi = {10.1007/s00216-015-8460-5},
url = {https://www.osti.gov/biblio/1226917}, journal = {Analytical and Bioanalytical Chemistry},
issn = {1618-2642},
number = 8,
volume = 407,
place = {United States},
year = {Sun Jan 25 00:00:00 EST 2015},
month = {Sun Jan 25 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 96 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Compound and metabolite distribution measured by MALDI mass spectrometric imaging in whole-body tissue sections
journal, February 2007


Sublimation as a method of matrix application for mass spectrometric imaging
journal, September 2007


Dynamics of ions of intact proteins in the Orbitrap mass analyzer
journal, August 2009


Mass Spectrometric Imaging for Biomedical Tissue Analysis
journal, May 2010


MALDI Mass Spectrometry Imaging in Microscope Mode with Infrared Lasers: Bypassing the Diffraction Limits
journal, December 2013


Histology by Mass Spectrometry: Label-Free Tissue Characterization Obtained from High-Accuracy Bioanalytical Imaging
journal, April 2010


Laser Beam Filtration for High Spatial Resolution MALDI Imaging Mass Spectrometry
journal, May 2013


Implementation of a Gaussian Beam Laser and Aspheric Optics for High Spatial Resolution MALDI Imaging MS
journal, April 2014


Targeted Multiplex Imaging Mass Spectrometry in Transmission Geometry for Subcellular Spatial Resolution
journal, February 2013


MALDI-MS imaging of features smaller than the size of the laser beam
journal, October 2005


Detection of phosphoenolpyruvate and ribulose 1,5-bisphosphate carboxylase transcripts in maize leaves by in situ hybridization with sulfonated cDNA probes.
journal, April 1989


Multiplex MALDI-MS Imaging of Plant Metabolites Using a Hybrid MS System
book, October 2014


MALDI produced ions inspected with a linear ion trap-orbitrap hybrid mass analyzer
journal, August 2009


Determination of flavone, flavonol, and flavanone aglycones by negative ion liquid chromatography electrospray ion trap mass spectrometry
journal, June 2001


The Phenylpropanoid Pathway in Arabidopsis
journal, January 2011


Effect of Maysin on Wild-Type, Deltamethrin-Resistant, and Bt-Resistant Helicoverpa armigera (Lepidoptera: Noctuidae)
journal, June 2003


Phosphatidylglycerol and Chilling Sensitivity in Plants
journal, March 1985


Lipid molecular species composition of thylakoid membranes
journal, January 1980


Role of natural benzoxazinones in the survival strategy of plants
book, January 2000


What role does sulpholipid play within the thylakoid membrane?
journal, January 1986


What role does sulpholipid play within the thylakoid membrane?
book, January 1986


Effect of Maysin on Wild-Type, Deltamethrin-Resistant, and Bt-Resistant Helicoverpa armigera (Lepidoptera: Noctuidae)
journal, June 2003


Direct imaging of single cells and tissue at sub-cellular spatial resolution using transmission geometry MALDI MS
journal, November 2012


Works referencing / citing this record:

Study of the cyanoacrylate fuming mechanism by matrix-assisted laser desorption/ionization mass spectrometry
journal, February 2019


Confirmation of sub-cellular resolution using oversampling imaging mass spectrometry
journal, November 2019


Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation
journal, December 2019


Mass spectrometry imaging for plant biology: a review
journal, October 2015


Plant defense and herbivore counter-defense: benzoxazinoids and insect herbivores
journal, November 2016


Metabotyping of 30 maize hybrids under early-sowing conditions reveals potential marker-metabolites for breeding
journal, September 2018


microMS: A Python Platform for Image-Guided Mass Spectrometry Profiling
journal, June 2017


3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish
journal, November 2017


Subcellular Peptide Localization in Single Identified Neurons by Capillary Microsampling Mass Spectrometry
journal, August 2018


Assimilation of ‘omics’ strategies to study the cuticle layer and suberin lamellae in plants
journal, October 2017


Chemical Imaging of Cyanoacrylate-Fumed Fingerprints by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Imaging,
journal, March 2018


Applications of Fourier Transform Ion Cyclotron Resonance (FT-ICR) and Orbitrap Based High Resolution Mass Spectrometry in Metabolomics and Lipidomics
journal, May 2016


Mass spectrometry imaging for plant biology: a review
journal, October 2015


Plant defense and herbivore counter-defense: benzoxazinoids and insect herbivores
journal, November 2016


Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
journal, January 2020


3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish
journal, November 2017


Sample Preparation for Mass Spectrometry Imaging of Plant Tissues: A Review
journal, February 2016


Applications of Fourier Transform Ion Cyclotron Resonance (FT-ICR) and Orbitrap Based High Resolution Mass Spectrometry in Metabolomics and Lipidomics
journal, May 2016