Rapid microfluidic thermal cycler for nucleic acid amplification

Beer, Neil Reginald; Vafai, Kambiz

Title: Rapid microfluidic thermal cycler for nucleic acid amplification

Patent · Tue Oct 27 00:00:00 EDT 2015

OSTI ID:1224444

Beer, Neil Reginald; Vafai, Kambiz

A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-07NA27344

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Number(s):: 9,170,060

Application Number:: 12/270,348

OSTI ID:: 1224444

Resource Relation:: Patent File Date: 2008 Nov 13

Country of Publication:: United States

Language:: English

References (15)

Process for amplifying, detecting, and/or-cloning nucleic acid sequences Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683195 US Patent Document 4,683,195	patent	July 1987
Process for amplifying nucleic acid sequences Mullis, Kary B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683202 US Patent Document 4,683,202	patent	July 1987
End-complementary polymerase reaction Stemmer, Willem P.; Lipshutz, Robert J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5834252 US Patent Document 5,834,252	patent	November 1998
Method for recombining polynucleotides Slater, Steven C.; Ng, Wing-On; Feng, Guo Hong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020119535 US Patent Application 09/746432; 20020119535	patent-application	August 2002
Method for the complete chemical synthesis and assembly of genes and genomes Evans, Glen A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030165946 US Patent Application 10/322360; 20030165946	patent-application	September 2003
Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control Atwood, John Girdner; Mossa, Albert Carmelo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040086927 US Patent Application 10/691186; 20040086927	patent-application	June 2004
Thermal reaction device and method for using the same McBride, Lincoln; Facer, Geoffrey; Unger, Marc https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050252773 US Patent Application 11/084357; 20050252773	patent-application	November 2005
Method for carrying out a biochemical protocol in continuous flow in a microreactor Fouillet, Yves; Vauchier, Claude; Clerc, Jean-Frederic https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050282224 US Patent Application 11/197040; 20050282224	patent-application	December 2005
Pumped fluid cooling system and method Werner, Douglas; Goodson, Kenneth; Munch, Mark https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060042785 US Patent Application 10/927800; 20060042785	patent-application	March 2006
Thermal cycler for microfluidic array assays Yoder, Karl; Brenan, Colin; Linton, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060094108 US Patent Application 11/227425; 20060094108	patent-application	May 2006
Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture Beer, Neil; Hindson, Benjamin; Colson, Billy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080166793 US Patent Application 11/650363; 20080166793	patent-application	July 2008
Portable Rapid Microfluidic Thermal Cycler for Extremely Fast Nucleic Acid Amplification Beer, Neil Reginald; Vafai, Kambiz; Mahjoob, Shadi https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090226971 US Patent Application 12/270030; 20090226971	patent-application	September 2009
Isothermal surface production and regulation for high heat flux applications utilizing porous inserts Khanafer, K.; Vafai, K. International Journal of Heat and Mass Transfer, Vol. 44, Issue 15, p. 2933-2947 https://doi.org/10.1016/S0017-9310(00)00336-7	journal	August 2001
Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification Mahjoob, Shadi; Vafai, Kambiz; Beer, N. Reginald International Journal of Heat and Mass Transfer, Vol. 51, Issue 9-10, p. 2109-2122 https://doi.org/10.1016/j.ijheatmasstransfer.2007.11.014	journal	May 2008
On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858 https://doi.org/10.1021/ac800048k	journal	March 2008

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