skip to main content

SciTech ConnectSciTech Connect

Title: Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is a pertinent step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [1] ;  [1] ;  [4] ;  [4] ;  [4] ;  [4] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [5] ;  [1]
  1. Arizona State Univ., Tempe, AZ (United States)
  2. State Univ. of New York, Buffalo, NY (United States)
  3. Arizona State Univ., Tempe, AZ (United States); Beijing Computational Science Research Center, Beijing (China)
  4. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  5. Univ. of Southern California, Los Angeles, CA (United States)
Publication Date:
OSTI Identifier:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Structural Dynamics
Additional Journal Information:
Journal Volume: 2; Journal Issue: 4; Journal ID: ISSN 2329-7778
American Crystallographic Association/AIP
Research Org:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES viruses; x-ray scattering; proteins; x-ray diffraction; crystal structure