Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

Schostag, Morten; Stibal, Marek; Jacobsen, Carsten S.; Baelum, Jacob; Tas, Neslihan; Elberling, Bo; Jansson, Janet K.; Semenchuk, Phillip; Prieme, Anders

doi:10.3389/fmicb.2015.00399

Title: Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

Abstract

The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below -10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soilmore »« less

Authors:: Schostag, Morten; Stibal, Marek; Jacobsen, Carsten S.; Baelum, Jacob; Tas, Neslihan; Elberling, Bo; Jansson, Janet K.; Semenchuk, Phillip; Prieme, Anders

Publication Date:: Thu Apr 30 00:00:00 EDT 2015

Research Org.:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1221483

Report Number(s):: PNNL-SA-110669

DOE Contract Number:: AC05-76RL01830

Resource Type:: Journal Article

Journal Name:: Frontiers in Microbiology, 6:Article No. 399

Additional Journal Information:: Journal Name: Frontiers in Microbiology, 6:Article No. 399

Country of Publication:: United States

Language:: English

Subject:: permafrost; arctic; bacterial communities; Alphaproteobacteria; Cyanobacteria; phototrophic organisms

Citation Formats


                    Schostag, Morten, Stibal, Marek, Jacobsen, Carsten S., Baelum, Jacob, Tas, Neslihan, Elberling, Bo, Jansson, Janet K., Semenchuk, Phillip, and Prieme, Anders. Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses.  United States: N. p., 2015. 
        Web.  doi:10.3389/fmicb.2015.00399.

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                    Schostag, Morten, Stibal, Marek, Jacobsen, Carsten S., Baelum, Jacob, Tas, Neslihan, Elberling, Bo, Jansson, Janet K., Semenchuk, Phillip, & Prieme, Anders. Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses.  United States.  https://doi.org/10.3389/fmicb.2015.00399

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                    Schostag, Morten, Stibal, Marek, Jacobsen, Carsten S., Baelum, Jacob, Tas, Neslihan, Elberling, Bo, Jansson, Janet K., Semenchuk, Phillip, and Prieme, Anders. 2015.  
        "Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses".  United States.  https://doi.org/10.3389/fmicb.2015.00399.

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@article{osti_1221483,

  title        = {Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses},

  author       = {Schostag, Morten and Stibal, Marek and Jacobsen, Carsten S. and Baelum, Jacob and Tas, Neslihan and Elberling, Bo and Jansson, Janet K. and Semenchuk, Phillip and Prieme, Anders},

  abstractNote = {The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below -10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface.},

  doi          = {10.3389/fmicb.2015.00399},

  url          = {https://www.osti.gov/biblio/1221483},
  journal      = {Frontiers in Microbiology, 6:Article No. 399},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Apr 30 00:00:00 EDT 2015},

  month        = {Thu Apr 30 00:00:00 EDT 2015}

}

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https://doi.org/10.3389/fmicb.2016.00772

Diversity and Composition of Bacterial Community in Soils and Lake Sediments from an Arctic Lake Area
journal, July 2016

Wang, Neng Fei; Zhang, Tao; Yang, Xiao
Frontiers in Microbiology, Vol. 7
https://doi.org/10.3389/fmicb.2016.01170

Microbial dynamics in a High Arctic glacier forefield: a combined field, laboratory, and modelling approach
journal, January 2016

Bradley, James A.; Arndt, Sandra; Šabacká, Marie
Biogeosciences, Vol. 13, Issue 19
https://doi.org/10.5194/bg-13-5677-2016

Linkages between geochemistry and microbiology in a proglacial terrain in the High Arctic
text, January 2018

Wojcik, Robin; Donhauser, Johanna; Frey, Beat
Freie Universität Berlin
https://doi.org/10.17169/refubium-2750

The subzero microbiome: Microbial activity in frozen and thawing soils
text, January 2017

Nikrad, Mrinalini P.; Kerkhof, Lee J.; Häggblom, Max M.
Rutgers University
https://doi.org/10.7282/t3571f35

Microbial diversity in European alpine permafrost and active layers
text, January 2016

Beat, Frey,; Thomas, Rime,; Marcia, Phillips,
ETH Zurich
https://doi.org/10.3929/ethz-b-000114910

Influence of glaciation on mechanisms of mineral weathering in two high Arctic catchments
journal, January 2016

Hindshaw, Ruth S.; Heaton, Tim H. E.; Boyd, Eric S.
Chemical Geology, Vol. 420
https://doi.org/10.1016/j.chemgeo.2015.11.004

Erratum: Potential microbial contamination during sampling of permafrost soil assessed by tracers
journal, April 2017

Bang-Andreasen, Toke; Schostag, Morten; Priemé, Anders
Scientific Reports, Vol. 7, Issue 1
https://doi.org/10.1038/srep46006

Emerging from the ice‐fungal communities are diverse and dynamic in earliest soil developmental stages of a receding glacier
journal, March 2019

Dresch, Philipp; Falbesoner, Johannes; Ennemoser, Chiara
Environmental Microbiology, Vol. 21, Issue 5
https://doi.org/10.1111/1462-2920.14598

Rare Taxa Exhibit Disproportionate Cell-Level Metabolic Activity in Enriched Anaerobic Digestion Microbial Communities
journal, January 2019

Jia, Yangyang; Leung, Marcus H. Y.; Tong, Xinzhao
mSystems, Vol. 4, Issue 1
https://doi.org/10.1128/msystems.00208-18

A comparison of methods used to unveil the genetic and metabolic pool in the built environment
journal, April 2018

Gomez-Silvan, Cinta; Leung, Marcus H. Y.; Grue, Katherine A.
Microbiome, Vol. 6, Issue 1
https://doi.org/10.1186/s40168-018-0453-0

Bacterial Community Structures in Freshwater Polar Environments of Svalbard
journal, January 2016

Ntougias, Spyridon; Polkowska, Żaneta; Nikolaki, Sofia
Microbes and Environments, Vol. 31, Issue 4
https://doi.org/10.1264/jsme2.me16074

Diversity and Composition of Bacterial Community in Soils and Lake Sediments from an Arctic Lake Area
journal, July 2016

Wang, Neng Fei; Zhang, Tao; Yang, Xiao
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https://doi.org/10.3389/fmicb.2016.01170

Microbial Communities and Diversities in Mudflat Sediments Analyzed Using a Modified Metatranscriptomic Method
journal, January 2018

Yan, Yong-Wei; Jiang, Qiu-Yue; Wang, Jian-Gong
Frontiers in Microbiology, Vol. 9
https://doi.org/10.3389/fmicb.2018.00093

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Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

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The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy numbermore »« less
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Title: Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses

Abstract

Citation Formats

Global analysis of nitrogen and phosphorus limitation dataset, January 2007

Effect of Freeze-Thaw Cycles on Bacterial Communities of Arctic Tundra Soil journal, April 2009

Deepened snow alters soil microbial nutrient limitations in arctic birch hummock tundra journal, June 2008

Seasonal dynamics of nutrients and bacterial communities in unvegetated alpine glacier forefields journal, February 2012

A surface-mediated origin of the RNA world: biogenic activities of clay-adsorbed RNA molecules journal, February 2005

Late snowmelt delays plant development and results in lower reproductive success in the High Arctic journal, January 2011

Bacterial communities in tetrachloroethene-polluted groundwaters: A case study journal, June 2013

A cross-seasonal comparison of active and total bacterial community composition in Arctic tundra soil using bromodeoxyuridine labeling journal, February 2011

The seasonal pattern of soil microbial community structure in mesic low arctic tundra journal, October 2013

Soil fertility is associated with fungal and bacterial richness, whereas pH is associated with community composition in polar soil microbial communities journal, November 2014

Temperature-dependent changes in the soil bacterial community in limed and unlimed soil journal, July 2003

Biogeochemistry of Metalliferous Peats: Sulfur Speciation and Depth Distributions of dsr AB Genes and Cd, Fe, Mn, S, and Zn in Soil Cores journal, August 2007

Pyrosequencing enumerates and contrasts soil microbial diversity journal, July 2007

A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses journal, January 2009

Despite strong seasonal responses, soil microbial consortia are more resilient to long-term changes in rainfall than overlying grassland journal, March 2009

Seasonality and resource availability control bacterial and archaeal communities in soils of a temperate beech forest journal, September 2010

The functional potential of high Arctic permafrost revealed by metagenomic sequencing, qPCR and microarray analyses journal, April 2010

Selection of primers for optimal taxonomic classification of environmental 16S rRNA gene sequences journal, January 2012

Active and total microbial communities in forest soil are largely different and highly stratified during decomposition journal, July 2011

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms journal, March 2012

Organic carbon transformations in high-Arctic peat soils: key functions and microorganisms journal, September 2012

Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses journal, July 2013

Bacterial genome replication at subzero temperatures in permafrost journal, August 2013

Distinct microbial communities associated with buried soils in the Siberian tundra journal, December 2013

Bacterial growth at −15 °C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1 journal, February 2013

The effect of permafrost thaw on old carbon release and net carbon exchange from tundra journal, May 2009

Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw journal, November 2011

Past extreme warming events linked to massive carbon release from thawing permafrost journal, April 2012

Multi-omics of permafrost, active layer and thermokarst bog soil microbiomes journal, March 2015

Plot-scale evidence of tundra vegetation change and links to recent summer warming journal, April 2012

Long-term CO2 production following permafrost thaw journal, July 2013

Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing journal, December 2012

UPARSE: highly accurate OTU sequences from microbial amplicon reads journal, August 2013

Infant gut microbiota characteristics generally do not modify effects of lipid-based nutrient supplementation on growth or inflammation: secondary analysis of a randomized controlled trial in Malawi journal, September 2020

Ancient bacteria show evidence of DNA repair journal, August 2007

Statistics and characteristics of permafrost and ground-ice distribution in the Northern Hemisphere journal, March 2008

UCHIME improves sensitivity and speed of chimera detection journal, June 2011

Design and Experimental Application of a Novel Non-Degenerate Universal Primer Set that Amplifies Prokaryotic 16S rRNA Genes with a Low Possibility to Amplify Eukaryotic rRNA Genes journal, November 2013

Rhodoplanes gen. nov., a New Genus of Phototrophic Bacteria Including Rhodopseudomonas rosea as Rhodoplanes roseus comb. nov. and Rhodoplanes elegans sp. nov. journal, October 1994

Rhodoplanes serenus sp. nov., a purple non-sulfur bacterium isolated from pond water journal, March 2009

Rhodoplanes pokkaliisoli sp. nov., a phototrophic alphaproteobacterium isolated from a waterlogged brackish paddy soil journal, July 2009

Granulicella paludicola gen. nov., sp. nov., Granulicella pectinivorans sp. nov., Granulicella aggregans sp. nov. and Granulicella rosea sp. nov., acidophilic, polymer-degrading acidobacteria from Sphagnum peat bogs journal, January 2010

Rhodoplanes piscinae sp. nov. isolated from pond water journal, December 2012

Rhodoplanes oryzae sp. nov., a phototrophic alphaproteobacterium isolated from the rhizosphere soil of paddy journal, July 2014

Acidobacteria dominate the active bacterial communities of Arctic tundra with widely divergent winter-time snow accumulation and soil temperatures journal, November 2012

Bacterial community structure and soil properties of a subarctic tundra soil in Council, Alaska journal, August 2014

Bacterial responses to fluctuations and extremes in temperature and brine salinity at the surface of Arctic winter sea ice journal, June 2014

Predicting long-term carbon mineralization and trace gas production from thawing permafrost of Northeast Siberia journal, January 2013

Water availability controls microbial temperature responses in frozen soil CO2 production: FROZEN SOILS AND UNFROZEN WATER journal, November 2009

Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes journal, February 2012

Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems journal, December 2007

Transcription dynamics of the functional tfdA gene during MCPA herbicide degradation by Cupriavidus necator AEO106 (pRO101) in agricultural soil journal, March 2008

Bacterial and fungal community structure in Arctic tundra tussock and shrub soils: Community structure in Arctic tundra tussock and shrub soils journal, February 2007

Multivariate analyses in microbial ecology: Multivariate analyses in microbial ecology journal, November 2007

Soil-specific limitations for access and analysis of soil microbial communities by metagenomics: Limitations in soil metagenomics journal, June 2011

Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils journal, April 2012

Effects of season and experimental warming on the bacterial community in a temperate mountain forest soil assessed by 16S rRNA gene pyrosequencing journal, June 2012

Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella journal, April 2004

Functional Ecology of Free-Living Nitrogen Fixation: A Contemporary Perspective journal, December 2011

Spatial and Temporal Variation of Archaeal, Bacterial and Fungal Communities in Agricultural Soils journal, December 2012

The Effect of Primer Choice and Short Read Sequences on the Outcome of 16S rRNA Gene Based Diversity Studies journal, August 2013

Toward an Ecological Classification of soil Bacteria journal, June 2007

Consistent effects of nitrogen fertilization on soil bacterial communities in contrasting systems journal, December 2010

Soil bacterial community composition altered by increased nutrient availability in Arctic tundra soils journal, October 2014

Multivariate analyses in microbial ecology text, January 2007

Bacterial communities in ancient permafrost profiles of Svalbard, Arctic journal, September 2017

Linkages between geochemistry and microbiology in a proglacial terrain in the High Arctic journal, December 2018

Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil journal, January 2019

Microbial diversity in European alpine permafrost and active layers journal, January 2016

The subzero microbiome: microbial activity in frozen and thawing soils journal, April 2016

Aeolian dispersal of bacteria in southwest Greenland: their sources, abundance, diversity and physiological states journal, February 2018

Disentangling the complexity of permafrost soil by using high resolution profiling of microbial community composition, key functions and respiration rates journal, July 2018

Emerging from the ice‐fungal communities are diverse and dynamic in earliest soil developmental stages of a receding glacier journal, March 2019

Microbial diversity and biogeography in Arctic soils: Microbial diversity and biogeography journal, August 2018

Rare Taxa Exhibit Disproportionate Cell-Level Metabolic Activity in Enriched Anaerobic Digestion Microbial Communities journal, January 2019

A comparison of methods used to unveil the genetic and metabolic pool in the built environment journal, April 2018

Molecular Evidence for Metabolically Active Bacteria in the Atmosphere journal, May 2016

Global analysis of nitrogen and phosphorus limitation
dataset, January 2007

Effect of Freeze-Thaw Cycles on Bacterial Communities of Arctic Tundra Soil
journal, April 2009

Deepened snow alters soil microbial nutrient limitations in arctic birch hummock tundra
journal, June 2008

Seasonal dynamics of nutrients and bacterial communities in unvegetated alpine glacier forefields
journal, February 2012

A surface-mediated origin of the RNA world: biogenic activities of clay-adsorbed RNA molecules
journal, February 2005

Late snowmelt delays plant development and results in lower reproductive success in the High Arctic
journal, January 2011

Bacterial communities in tetrachloroethene-polluted groundwaters: A case study
journal, June 2013

A cross-seasonal comparison of active and total bacterial community composition in Arctic tundra soil using bromodeoxyuridine labeling
journal, February 2011

The seasonal pattern of soil microbial community structure in mesic low arctic tundra
journal, October 2013

Soil fertility is associated with fungal and bacterial richness, whereas pH is associated with community composition in polar soil microbial communities
journal, November 2014

Temperature-dependent changes in the soil bacterial community in limed and unlimed soil
journal, July 2003

Biogeochemistry of Metalliferous Peats: Sulfur Speciation and Depth Distributions of dsr AB Genes and Cd, Fe, Mn, S, and Zn in Soil Cores
journal, August 2007

Pyrosequencing enumerates and contrasts soil microbial diversity
journal, July 2007

A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses
journal, January 2009

Despite strong seasonal responses, soil microbial consortia are more resilient to long-term changes in rainfall than overlying grassland
journal, March 2009

Seasonality and resource availability control bacterial and archaeal communities in soils of a temperate beech forest
journal, September 2010

The functional potential of high Arctic permafrost revealed by metagenomic sequencing, qPCR and microarray analyses
journal, April 2010

Selection of primers for optimal taxonomic classification of environmental 16S rRNA gene sequences
journal, January 2012

Active and total microbial communities in forest soil are largely different and highly stratified during decomposition
journal, July 2011

Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012

Organic carbon transformations in high-Arctic peat soils: key functions and microorganisms
journal, September 2012

Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses
journal, July 2013

Bacterial genome replication at subzero temperatures in permafrost
journal, August 2013

Distinct microbial communities associated with buried soils in the Siberian tundra
journal, December 2013

Bacterial growth at −15 °C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1
journal, February 2013

The effect of permafrost thaw on old carbon release and net carbon exchange from tundra
journal, May 2009

Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw
journal, November 2011

Past extreme warming events linked to massive carbon release from thawing permafrost
journal, April 2012

Multi-omics of permafrost, active layer and thermokarst bog soil microbiomes
journal, March 2015

Plot-scale evidence of tundra vegetation change and links to recent summer warming
journal, April 2012

Long-term CO2 production following permafrost thaw
journal, July 2013

Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing
journal, December 2012

UPARSE: highly accurate OTU sequences from microbial amplicon reads
journal, August 2013

Infant gut microbiota characteristics generally do not modify effects of lipid-based nutrient supplementation on growth or inflammation: secondary analysis of a randomized controlled trial in Malawi
journal, September 2020

Ancient bacteria show evidence of DNA repair
journal, August 2007

Statistics and characteristics of permafrost and ground-ice distribution in the Northern Hemisphere
journal, March 2008

UCHIME improves sensitivity and speed of chimera detection
journal, June 2011

Design and Experimental Application of a Novel Non-Degenerate Universal Primer Set that Amplifies Prokaryotic 16S rRNA Genes with a Low Possibility to Amplify Eukaryotic rRNA Genes
journal, November 2013

Rhodoplanes gen. nov., a New Genus of Phototrophic Bacteria Including Rhodopseudomonas rosea as Rhodoplanes roseus comb. nov. and Rhodoplanes elegans sp. nov.
journal, October 1994

Rhodoplanes serenus sp. nov., a purple non-sulfur bacterium isolated from pond water
journal, March 2009

Rhodoplanes pokkaliisoli sp. nov., a phototrophic alphaproteobacterium isolated from a waterlogged brackish paddy soil
journal, July 2009

Granulicella paludicola gen. nov., sp. nov., Granulicella pectinivorans sp. nov., Granulicella aggregans sp. nov. and Granulicella rosea sp. nov., acidophilic, polymer-degrading acidobacteria from Sphagnum peat bogs
journal, January 2010

Rhodoplanes piscinae sp. nov. isolated from pond water
journal, December 2012

Rhodoplanes oryzae sp. nov., a phototrophic alphaproteobacterium isolated from the rhizosphere soil of paddy
journal, July 2014

Acidobacteria dominate the active bacterial communities of Arctic tundra with widely divergent winter-time snow accumulation and soil temperatures
journal, November 2012

Bacterial community structure and soil properties of a subarctic tundra soil in Council, Alaska
journal, August 2014

Bacterial responses to fluctuations and extremes in temperature and brine salinity at the surface of Arctic winter sea ice
journal, June 2014

Predicting long-term carbon mineralization and trace gas production from thawing permafrost of Northeast Siberia
journal, January 2013

Water availability controls microbial temperature responses in frozen soil CO2 production: FROZEN SOILS AND UNFROZEN WATER
journal, November 2009

Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes
journal, February 2012

Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems
journal, December 2007

Transcription dynamics of the functional tfdA gene during MCPA herbicide degradation by Cupriavidus necator AEO106 (pRO101) in agricultural soil
journal, March 2008

Bacterial and fungal community structure in Arctic tundra tussock and shrub soils: Community structure in Arctic tundra tussock and shrub soils
journal, February 2007

Multivariate analyses in microbial ecology: Multivariate analyses in microbial ecology
journal, November 2007

Soil-specific limitations for access and analysis of soil microbial communities by metagenomics: Limitations in soil metagenomics
journal, June 2011

Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils
journal, April 2012

Effects of season and experimental warming on the bacterial community in a temperate mountain forest soil assessed by 16S rRNA gene pyrosequencing
journal, June 2012

Relationship of Critical Temperature to Macromolecular Synthesis and Growth Yield in Psychrobacter cryopegella
journal, April 2004

Functional Ecology of Free-Living Nitrogen Fixation: A Contemporary Perspective
journal, December 2011

Spatial and Temporal Variation of Archaeal, Bacterial and Fungal Communities in Agricultural Soils
journal, December 2012

The Effect of Primer Choice and Short Read Sequences on the Outcome of 16S rRNA Gene Based Diversity Studies
journal, August 2013

Toward an Ecological Classification of soil Bacteria
journal, June 2007

Consistent effects of nitrogen fertilization on soil bacterial communities in contrasting systems
journal, December 2010

Soil bacterial community composition altered by increased nutrient availability in Arctic tundra soils
journal, October 2014

Multivariate analyses in microbial ecology
text, January 2007

Bacterial communities in ancient permafrost profiles of Svalbard, Arctic
journal, September 2017

Linkages between geochemistry and microbiology in a proglacial terrain in the High Arctic
journal, December 2018

Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil
journal, January 2019

Microbial diversity in European alpine permafrost and active layers
journal, January 2016

The subzero microbiome: microbial activity in frozen and thawing soils
journal, April 2016

Aeolian dispersal of bacteria in southwest Greenland: their sources, abundance, diversity and physiological states
journal, February 2018

Disentangling the complexity of permafrost soil by using high resolution profiling of microbial community composition, key functions and respiration rates
journal, July 2018

Emerging from the ice‐fungal communities are diverse and dynamic in earliest soil developmental stages of a receding glacier
journal, March 2019

Microbial diversity and biogeography in Arctic soils: Microbial diversity and biogeography
journal, August 2018

Rare Taxa Exhibit Disproportionate Cell-Level Metabolic Activity in Enriched Anaerobic Digestion Microbial Communities
journal, January 2019

A comparison of methods used to unveil the genetic and metabolic pool in the built environment
journal, April 2018

Molecular Evidence for Metabolically Active Bacteria in the Atmosphere
journal, May 2016

Diversity and Composition of Bacterial Community in Soils and Lake Sediments from an Arctic Lake Area
journal, July 2016

Microbial dynamics in a High Arctic glacier forefield: a combined field, laboratory, and modelling approach
journal, January 2016

Linkages between geochemistry and microbiology in a proglacial terrain in the High Arctic
text, January 2018