Fluorescence dye tagging scheme for mercury quantification and speciation
Abstract
A fluorescent dye or fluorophore capable of forming complexes with mercury comprises 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate amide, wherein the amide is formed by reacting the succinimidyl ester (Pacific Blue.TM.) with an amino acid containing a thiol group, such as cysteine or glutathione. Mercury complexes of the fluorophore fluoresce when excited by a UV or violet laser diode, and the detected intensity can be calibrated to quantify the concentration of mercury in a sample reacted with the fluorophore.
- Inventors:
- Publication Date:
- Research Org.:
- Los Gatos Research, Mountain View, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1221386
- Patent Number(s):
- 9,139,550
- Application Number:
- 13/570,548
- Assignee:
- Los Gatos Research (Mountain View, CA)
- DOE Contract Number:
- SC0004430
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Aug 09
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Jiao, Hong, and Catterall, Hannah. Fluorescence dye tagging scheme for mercury quantification and speciation. United States: N. p., 2015.
Web.
Jiao, Hong, & Catterall, Hannah. Fluorescence dye tagging scheme for mercury quantification and speciation. United States.
Jiao, Hong, and Catterall, Hannah. 2015.
"Fluorescence dye tagging scheme for mercury quantification and speciation". United States. https://www.osti.gov/servlets/purl/1221386.
@article{osti_1221386,
title = {Fluorescence dye tagging scheme for mercury quantification and speciation},
author = {Jiao, Hong and Catterall, Hannah},
abstractNote = {A fluorescent dye or fluorophore capable of forming complexes with mercury comprises 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate amide, wherein the amide is formed by reacting the succinimidyl ester (Pacific Blue.TM.) with an amino acid containing a thiol group, such as cysteine or glutathione. Mercury complexes of the fluorophore fluoresce when excited by a UV or violet laser diode, and the detected intensity can be calibrated to quantify the concentration of mercury in a sample reacted with the fluorophore.},
doi = {},
url = {https://www.osti.gov/biblio/1221386},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 22 00:00:00 EDT 2015},
month = {Tue Sep 22 00:00:00 EDT 2015}
}
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