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Title: Complete genome sequence of Pelosinus sp. strain UFO1 assembled using single-molecule real-time DNA sequencing technology

Journal Article · · Genome Announcements
 [1];  [2];  [3];  [4];  [5];  [5];  [5];  [5];  [6]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States)
  3. Idaho State Univ., Pocatello, ID (United States)
  4. Idaho National Lab. (INL), Idaho Falls, ID (United States)
  5. Univ. of Georgia, Athens, GA (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
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Pelosinus fermentans strain R7 was isolated from Russian kaolin clays as the type strain and it can reduce Fe(III) during fermentative growth (1). Draft genome sequences for P. fermentans R7 and four strains from Hanford, Washington, USA, have been published (2–4). The P. fermentans 16S rRNA sequence dominated the lactate-based enrichment cultures from three geochemically contrasting soils from the Melton Branch Watershed, Oak Ridge, Tennessee, USA (5) and also at another stimulated, uraniumcontaminated field site near Oak Ridge (6). For the current work, strain UFO1 was isolated from pristine sediments at a background field site in Oak Ridge and characterized as facilitating U(VI) reduction and precipitation with phosphate (7).

Research Organization:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231; AC07-05ID14517; AC05-00OR22725
OSTI ID:
1215523
Report Number(s):
INL/JOU-14-33908
Journal Information:
Genome Announcements, Vol. 2, Issue 5; ISSN 2169-8287
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

References (18)

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rRNA operon multiplicity in Escherichia coli and the physiological implications of rrn inactivation. journal January 1995
Repetitive DNA and next-generation sequencing: computational challenges and solutions journal November 2011
Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses journal January 2007
Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia journal January 2014
Evaluation and validation of de novo and hybrid assembly techniques to derive high-quality genome sequences journal June 2014
Complete Genome Sequence of Serratia liquefaciens Strain ATCC 27592 journal June 2013
Evidence for multiple modes of uranium immobilization by an anaerobic bacterium journal May 2011
Intragenomic heterogeneity of the 16S rRNA gene in strain UFO1 caused by a 100-bp insertion in helix 6: Intragenomic heterogeneity in helix 6 of UFO1 16S rRNA gene journal March 2010
Reducing assembly complexity of microbial genomes with single-molecule sequencing journal January 2013
Genomic and Physiological Characterization of the Chromate-Reducing, Aquifer-Derived Firmicute Pelosinus sp. Strain HCF1 journal October 2012
Draft Genome Sequences for Two Metal-Reducing Pelosinus fermentans Strains Isolated from a Cr(VI)-Contaminated Site and for Type Strain R7 journal August 2012
Draft Genome Sequence of Pelosinus fermentans JBW45, Isolated during In Situ Stimulation for Cr(VI) Reduction journal September 2012
Changes in Bacterial and Archaeal Community Structure and Functional Diversity along a Geochemically Variable Soil Profile journal January 2008
A Limited Microbial Consortium Is Responsible for Extended Bioreduction of Uranium in a Contaminated Aquifer journal July 2011
Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology journal March 2014
De Novo Assembly of the Streptomyces sp. Strain Mg1 Genome Using PacBio Single-Molecule Sequencing journal June 2013
rRNA Operon Copy Number Reflects Ecological Strategies of Bacteria journal April 2000

Cited By (7)

Investigating the Campylobacter jejuni Transcriptional Response to Host Intestinal Extracts Reveals the Involvement of a Widely Conserved Iron Uptake System journal August 2018
Scaffolding of a bacterial genome using MinION nanopore sequencing journal July 2015
A completeness-independent method for pre-selection of closely related genomes for species delineation in prokaryotes journal February 2020
Host-Microbiome Interaction and Cancer: Potential Application in Precision Medicine journal December 2016
Aquatic Macrophytes and Local Factors Drive Bacterial Community Distribution and Interactions in a Riparian Zone of Lake Taihu journal February 2020
Insights from 20 years of bacterial genome sequencing journal February 2015
A Case Study into Microbial Genome Assembly Gap Sequences and Finishing Strategies journal July 2017

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES/GENOMICS/GENOME RESEARCH
genome sequence
Pelosinus
strain UFO1