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Title: The Pacific Northwest National Laboratory library of bacterial and archaeal proteomic biodiversity

Journal Article · · Scientific Data, 2:Art. No. 150041
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  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Science Laboratory

This dataset deposition announces the submission to public repositories of the PNNL Biodiversity Library, a large collection of global proteomics data for 112 bacterial and archaeal organisms. The data comprises 35,162 tandem mass spectrometry (MS/MS) datasets from ~10 years of research. All data has been searched, annotated and organized in a consistent manner to promote reuse by the community. Protein identifications were cross-referenced with KEGG functional annotations which allows for pathway oriented investigation. We present the data as a freely available community resource. A variety of data re-use options are described for computational modeling, proteomics assay design and bioengineering. Instrument data and analysis files are available at ProteomeXchange via the MassIVE partner repository under the identifiers PXD001860 and MSV000079053.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC05-76RL01830
OSTI ID:
1213004
Report Number(s):
PNNL-SA-108944; 48582; KP1601010
Journal Information:
Scientific Data, 2:Art. No. 150041, Journal Name: Scientific Data, 2:Art. No. 150041
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

References (29)

Gene Expression Omnibus: NCBI gene expression and hybridization array data repository journal January 2002
PRISM: A data management system for high-throughput proteomics journal March 2006
Spectral archives: extending spectral libraries to analyze both identified and unidentified spectra journal May 2011
Comparative proteogenomics: Combining mass spectrometry and comparative genomics to analyze multiple genomes journal July 2008
A support vector machine model for the prediction of proteotypic peptides for accurate mass and time proteomics journal June 2010
Proteogenomic Analysis of Bacteria and Archaea: A 46 Organism Case Study journal November 2011
Comparative Bacterial Proteomics: Analysis of the Core Genome Concept journal February 2008
Does Trypsin Cut Before Proline? journal January 2008
The Proteomics Identifications (PRIDE) database and associated tools: status in 2013 journal November 2012
mzML—a Community Standard for Mass Spectrometry Data journal August 2010
Mass spectrometry-based proteomics journal March 2003
The Generating Function of CID, ETD, and CID/ETD Pairs of Tandem Mass Spectra: Applications to Database Search journal September 2010
The mzIdentML Data Standard for Mass Spectrometry-Based Proteomics Results journal February 2012
Estimating probabilities of correct identification from results of mass spectral library searches journal April 1994
Influence of Peptide Composition, Gas-Phase Basicity, and Chemical Modification on Fragmentation Efficiency:  Evidence for the Mobile Proton Model journal January 1996
Bifurcating Fragmentation Behavior of Gas-Phase Tryptic Peptide Dications in Collisional Activation journal December 2008
Computational prediction of proteotypic peptides for quantitative proteomics journal December 2006
Understanding the improved sensitivity of spectral library searching over sequence database searching in proteomics data analysis journal February 2011
Open Mass Spectrometry Search Algorithm journal October 2004
MyriMatch:  Highly Accurate Tandem Mass Spectral Peptide Identification by Multivariate Hypergeometric Analysis journal February 2007
Phosphorylation-Specific MS/MS Scoring for Rapid and Accurate Phosphoproteome Analysis journal August 2008
Mapping protein post-translational modifications with mass spectrometry journal September 2007
Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions journal May 2013
Accurate Annotation of Peptide Modifications through Unrestrictive Database Search journal January 2008
Phosphoproteome Analysis of E. coli Reveals Evolutionary Conservation of Bacterial Ser/Thr/Tyr Phosphorylation journal October 2007
A proteogenomic update to Yersinia: enhancing genome annotation journal August 2010
Ortho-proteogenomics: Multiple proteomes investigation through orthology and a new MS-based protocol journal October 2008
Using BiblioSpec for Creating and Searching Tandem MS Peptide Libraries journal December 2007
Building and Searching Tandem Mass Spectral Libraries for Peptide Identification journal September 2011

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