Quantitative characterization of collagen in the fibrotic capsule surrounding implanted polymeric microparticles through second harmonic generation imaging
The collagenous capsule formed around an implant will ultimately determine the nature of its in vivo fate. To provide a better understanding of how surface modifications can alter the collagen orientation and composition in the fibrotic capsule, we used second harmonic generation (SHG) microscopy to evaluate collagen organization and structure generated in mice subcutaneously injected with chemically functionalized polystyrene particles. SHG is sensitive to the orientation of a molecule, making it a powerful tool for measuring the alignment of collagen fibers. Additionally, SHG arises from the second order susceptibility of the interrogated molecule in response to the electric field. Variation in these tensor components distinguishes different molecular sources of SHG, providing collagen type specificity. Here, we demonstrated the ability of SHG to differentiate collagen type I and type III quantitatively and used this method to examine fibrous capsules of implanted polystyrene particles. Data presented in this work shows a wide range of collagen fiber orientations and collagen compositions in response to surface functionalized polystyrene particles. Dimethylamino functionalized particles were able to form a thin collagenous matrix resembling healthy skin. These findings have the potential to improve the fundamental understanding of how material properties influence collagen organization and composition quantitatively.
- Iowa State Univ., Ames, IA (United States). Dept. of Materials Science & Engineering.
- Iowa State Univ., Ames, IA, (United States). Dept. of Chemical & Biological Engineering; Ames National Laboratory, Ames, IA (United States)
- Pennsylvania State Hershey College of Medicine, Hershey, PA (United States)
- Publication Date:
- OSTI Identifier:
- Grant/Contract Number:
- W-7405-ENG-82; CBET 1227867; CMMI-0963224;
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 6; Journal ID: ISSN 1932-6203
- Public Library of Science
- Research Org:
- Ames Laboratory (AMES), Ames, IA (United States); National Science Foundation; Roy J. Carver Charitable Trust Grant No. 13-4265
- Sponsoring Org:
- USDOE Office of Science (SC)
- Country of Publication:
- United States
- 59 BASIC BIOLOGICAL SCIENCES
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