PR-PR: Cross-Platform Laboratory Automation System

Journal Article · Fri Aug 01 00:00:00 EDT 2014 · ACS SYNTHETIC BIOLOGY

DOI:https://doi.org/10.1021/sb4001728· OSTI ID:1211212

Linshiz, G; Stawski, N; Goyal, G; Bi, CH; Poust, S; Sharma, M; Mutalik, V; Keasling, JD; Hillson, NJ

To enable protocol standardization, sharing, and efficient implementation across laboratory automation platforms, we have further developed the PR-PR open-source high-level biology-friendly robot programming language as a cross-platform laboratory automation system. Beyond liquid-handling robotics, PR-PR now supports microfluidic and microscopy platforms, as well as protocol translation into human languages, such as English. While the same set of basic PR-PR commands and features are available for each supported platform, the underlying optimization and translation modules vary from platform to platform. Here, we describe these further developments to PR-PR, and demonstrate the experimental implementation and validation of PR-PR protocols for combinatorial modified Golden Gate DNA assembly across liquid-handling robotic, microfluidic, and manual platforms. To further test PR-PR cross-platform performance, we then implement and assess PR-PR protocols for Kunkel DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic and manual platforms.

• Sponsoring Organization: USDOE Advanced Research Projects Agency - Energy (ARPA-E)
• DOE Contract Number: DE-0000206-1577
• OSTI ID: 1211212
• Journal Information: ACS SYNTHETIC BIOLOGY, Vol. 3, Issue 8; ISSN 2161-5063
• Country of Publication: United States
• Language: English