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Title: Polypeptide having carbohydrate degrading activity and uses thereof

Patent ·
OSTI ID:1210881

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Research Organization:
DSM IP ASSETS B.V., Heerlen (Netherlands)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-08GO18079
Assignee:
DSM IP ASSETS B.V. (Heerlen, NL)
Patent Number(s):
9,109,213
Application Number:
13/806,132
OSTI ID:
1210881
Resource Relation:
Patent File Date: 2011 Jun 23
Country of Publication:
United States
Language:
English

References (10)

Nucleic acid and amino acid sequences relating to Apergillus fumigatus for diagnostics and therapeutics patent March 2009
Cellulose and ligno-cellulose active proteins patent May 2014
Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity journal December 2009
Molecular cloning and expression analysis of two distinct β-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii journal July 2007
Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii journal November 2004
Expression in Trichoderma reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus Talaromyces emersonii journal December 2004
Catalytic properties and mode of action of three endo-β-glucanases from Talaromyces emersonii on soluble β-1,4- and β-1,3;1,4-linked glucans journal November 2003
Molecular cloning, transcriptional, and expression analysis of the first cellulase gene (cbh2), encoding cellobiohydrolase II, from the moderately thermophilic fungus Talaromyces emersonii and structure prediction of the gene product journal February 2003
Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii journal November 2001
Stimulation of Lignocellulosic Biomass Hydrolysis by Proteins of Glycoside Hydrolase Family 61 Structure and Function of a Large, Enigmatic Family journal April 2010