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Title: Microfluidic device for acoustic cell lysis

Patent ·
OSTI ID:1207283
; ; ; ;

A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC04-94AL85000
Assignee:
Sandia Corporation (Albuquerque, NM)
Patent Number(s):
9,096,823
Application Number:
12/872,919
OSTI ID:
1207283
Resource Relation:
Patent File Date: 2010 Aug 31
Country of Publication:
United States
Language:
English

References (13)

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Acoustic Differential Extraction for Forensic Analysis of Sexual Assault Evidence journal August 2009
A Minisonicator To Rapidly Disrupt Bacterial Spores for DNA Analysis journal October 1999
Nucleic acid purification using microfabricated silicon structures journal October 2003
Continuous Spore Disruption Using Radially Focused, High-Frequency Ultrasound journal August 2001
Reversible capture of genomic DNA by a Nafion-coated electrode journal September 2008
Toward a microchip-based solid-phase extraction method for isolation of nucleic acids journal March 2002
Liquid Streaming by High-Frequency Ultrasonic Waves journal May 1996
Intelligent front-end sample preparation tool using acoustic streaming report September 2009
Miniaturized genetic analysis systems and methods patent January 2001
Microfluidic channels with integrated ultrasonic transducers for temperature measurement and method patent September 2004
Fluid-handling apparatus and methods patent June 2010
Apparatus to automatically lyse a sample patent August 2010

Cited By (1)

Microfluidic device for acoustic cell lysis patent August 2015

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