Development of a Process for the Spinning of Synthetic Spider Silk

Copeland, Cameron G.; Bell, Brianne E.; Christensen, Chad D.; Lewis, Randolph V.

doi:10.1021/acsbiomaterials.5b00092

Title: Development of a Process for the Spinning of Synthetic Spider Silk

Journal Article · Fri Jun 05 00:00:00 EDT 2015 · ACS Biomaterials Science & Engineering

DOI:https://doi.org/10.1021/acsbiomaterials.5b00092· OSTI ID:1203745

Copeland, Cameron G. ^[1]; Bell, Brianne E. ^[1]; Christensen, Chad D. ^[1]; Lewis, Randolph V. ^[1]

Utah State Univ., Logan, UT (United States). Dept. of Biological Engineering and Synthetic Biomanufacturing Center

Spider silks have unique mechanical properties but current efforts to duplicate those properties with recombinant proteins have been unsuccessful. Here, this study was designed to develop a single process to spin fibers with excellent and consistent mechanical properties. As-spun fibers produced were brittle, but by stretching the fibers the mechanical properties were greatly improved. A water-dip or water-stretch further increased the strength and elongation of the synthetic spider silk fibers. Given the promising results of the water stretch, a mechanical double-stretch system was developed. Both a methanol/water mixture and an isopropanol/water mixture were independently used to stretch the fibers with this system. We found that the methanol mixture produced fibers with high tensile strength while the isopropanol mixture produced fibers with high elongation.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF)

OSTI ID:: 1203745

Journal Information:: ACS Biomaterials Science & Engineering, Vol. 1, Issue 7; ISSN 2373-9878

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 57 works

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Recombinant spider silk from aqueous solutions via a bio-inspired microfluidic chip Peng, Qingfa; Zhang, Yaopeng; Lu, Li Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep36473	journal	November 2016
Method for the Destruction of Endotoxin in Synthetic Spider Silk Proteins Decker, Richard E.; Harris, Thomas I.; Memmott, Dylan R. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-29719-6	journal	August 2018
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3D nanofabrication inside rapid prototyped microfluidic channels showcased by wet-spinning of single micrometre fibres Lölsberg, Jonas; Linkhorst, John; Cinar, Arne Lab on a Chip, Vol. 18, Issue 9 https://doi.org/10.1039/c7lc01366c	journal	January 2018
Mechanically Strong Globular‐Protein‐Based Fibers Obtained Using a Microfluidic Spinning Technique He, Haonan; Yang, Chenjing; Wang, Fan Angewandte Chemie, Vol. 132, Issue 11 https://doi.org/10.1002/ange.201915262	journal	March 2020
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