FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis

Waadt, Rainer; Hitomi, Kenichi; Nishimura, Noriyuki; Hitomi, Chiharu; Adams, Stephen R.; Getzoff, Elizabeth D.; Schroeder, Julian I.

doi:10.7554/eLife.01739

Title: FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis

Journal Article · Tue Apr 15 00:00:00 EDT 2014 · eLife

DOI:https://doi.org/10.7554/eLife.01739· OSTI ID:1197664

Waadt, Rainer; Hitomi, Kenichi; Nishimura, Noriyuki; Hitomi, Chiharu; Adams, Stephen R.; Getzoff, Elizabeth D.; Schroeder, Julian I.

Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100–600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root.

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Research Organization:: Univ. of California, San Diego, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FG02-03ER15449

OSTI ID:: 1197664

Alternate ID(s):: OSTI ID: 1197665; OSTI ID: 1628805

Journal Information:: eLife, Journal Name: eLife Vol. 3; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 182 works

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References (90)

Generation of Active Pools of Abscisic Acid Revealed by In Vivo Imaging of Water-Stressed Arabidopsis Christmann, Alexander; Hoffmann, Thomas; Teplova, Irina Plant Physiology, Vol. 137, Issue 1 https://doi.org/10.1104/pp.104.053082	journal	December 2004
A gate–latch–lock mechanism for hormone signalling by abscisic acid receptors Melcher, Karsten; Ng, Ley-Moy; Zhou, X. Edward Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08613	journal	November 2009
A Brand New START: Abscisic Acid Perception and Transduction in the Guard Cell Joshi-Saha, A.; Valon, C.; Leung, J. Science Signaling, Vol. 4, Issue 201 https://doi.org/10.1126/scisignal.2002164	journal	November 2011
ABC transporter AtABCG25 is involved in abscisic acid transport and responses Kuromori, T.; Miyaji, T.; Yabuuchi, H. Proceedings of the National Academy of Sciences, Vol. 107, Issue 5 https://doi.org/10.1073/pnas.0912516107	journal	January 2010
Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair Geiger, Dietmar; Scherzer, Sönke; Mumm, Patrick Proceedings of the National Academy of Sciences, Vol. 106, Issue 50 https://doi.org/10.1073/pnas.0912021106	journal	December 2009
A hydraulic signal in root-to-shoot signalling of water shortage: Hydraulic signalling of water shortage Christmann, Alexander; Weiler, Elmar W.; Steudle, Ernst The Plant Journal, Vol. 52, Issue 1 https://doi.org/10.1111/j.1365-313X.2007.03234.x	journal	July 2007
Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins Park, S. -Y.; Fung, P.; Nishimura, N. Science https://doi.org/10.1126/science.1173041	journal	April 2009
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93% Goedhart, Joachim; von Stetten, David; Noirclerc-Savoye, Marjolaine Nature Communications, Vol. 3, Issue 1 https://doi.org/10.1038/ncomms1738	journal	January 2012
Structural Mechanism of Abscisic Acid Binding and Signaling by Dimeric PYR1 Nishimura, N.; Hitomi, K.; Arvai, A. S. Science, Vol. 326, Issue 5958 https://doi.org/10.1126/science.1181829	journal	October 2009
Action of Natural Abscisic Acid Precursors and Catabolites on Abscisic Acid Receptor Complexes Kepka, Michal; Benson, Chantel L.; Gonugunta, Vijay K. Plant Physiology, Vol. 157, Issue 4 https://doi.org/10.1104/pp.111.182584	journal	October 2011
ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves Tanaka, Yoko; Nose, Tomoe; Jikumaru, Yusuke The Plant Journal, Vol. 74, Issue 3 https://doi.org/10.1111/tpj.12136	journal	March 2013
Abscisic Acid: Emergence of a Core Signaling Network Cutler, Sean R.; Rodriguez, Pedro L.; Finkelstein, Ruth R. Annual Review of Plant Biology, Vol. 61, Issue 1 https://doi.org/10.1146/annurev-arplant-042809-112122	journal	June 2010
Activation of Glucosidase via Stress-Induced Polymerization Rapidly Increases Active Pools of Abscisic Acid Lee, Kwang Hee; Piao, Hai Lan; Kim, Ho-Youn Cell, Vol. 126, Issue 6 https://doi.org/10.1016/j.cell.2006.07.034	journal	September 2006
Structural basis of abscisic acid signalling Miyazono, Ken-ichi; Miyakawa, Takuya; Sawano, Yoriko Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08583	journal	October 2009
Protein Phosphatases 2C Regulate the Activation of the Snf1-Related Kinase OST1 by Abscisic Acid in Arabidopsis Vlad, Florina; Rubio, Silvia; Rodrigues, Americo The Plant Cell, Vol. 21, Issue 10 https://doi.org/10.1105/tpc.109.069179	journal	October 2009
Guard cell ABA and CO2 signaling network updates and Ca2+ sensor priming hypothesis Israelsson, Maria; Siegel, Robert S.; Young, Jared Current Opinion in Plant Biology, Vol. 9, Issue 6 https://doi.org/10.1016/j.pbi.2006.09.006	journal	December 2006
Complex Structures of the Abscisic Acid Receptor PYL3/RCAR13 Reveal a Unique Regulatory Mechanism Zhang, Xingliang; Zhang, Qi; Xin, Qi Structure, Vol. 20, Issue 5 https://doi.org/10.1016/j.str.2012.02.019	journal	May 2012
Water-stress-induced changes in the abscisic acid content of guard cells and other cells of Vicia faba L. leaves as determined by enzyme-amplified immunoassay Harris, M. J.; Outlaw, W. H.; Mertens, R. Proceedings of the National Academy of Sciences, Vol. 85, Issue 8 https://doi.org/10.1073/pnas.85.8.2584	journal	April 1988
Evolution of Abscisic Acid Synthesis and Signaling Mechanisms Hauser, Felix; Waadt, Rainer; Schroeder, Julian I. Current Biology, Vol. 21, Issue 9 https://doi.org/10.1016/j.cub.2011.03.015	journal	May 2011
Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase Sato, Aiko; Sato, Yuki; Fukao, Yoichiro Biochemical Journal, Vol. 424, Issue 3 https://doi.org/10.1042/BJ20091221	journal	December 2009
Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis Umezawa, T.; Sugiyama, N.; Mizoguchi, M. Proceedings of the National Academy of Sciences, Vol. 106, Issue 41 https://doi.org/10.1073/pnas.0907095106	journal	September 2009
A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress. Forcat, Silvia; Bennett, Mark H.; Mansfield, John W. Plant Methods, Vol. 4, Issue 1 https://doi.org/10.1186/1746-4811-4-16	journal	January 2008
Cameleon calcium indicator reports cytoplasmic calcium dynamics in Arabidopsis guard cells Allen, Gethyn J.; Kwak, June M.; Chu, Sarah P. The Plant Journal, Vol. 19, Issue 6 https://doi.org/10.1046/j.1365-313x.1999.00574.x	journal	September 1999
FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca2+ dynamics: Improved vectors for Ca2+ imaging in plants Krebs, Melanie; Held, Katrin; Binder, Andreas The Plant Journal, Vol. 69, Issue 1 https://doi.org/10.1111/j.1365-313X.2011.04780.x	journal	October 2011
The Molecular Basis of ABA-Independent Inhibition of PP2Cs by a Subclass of PYL Proteins Hao, Qi; Yin, Ping; Li, Wenqi Molecular Cell, Vol. 42, Issue 5 https://doi.org/10.1016/j.molcel.2011.05.011	journal	June 2011
The Arabidopsis ABA-Activated Kinase OST1 Phosphorylates the bZIP Transcription Factor ABF3 and Creates a 14-3-3 Binding Site Involved in Its Turnover Sirichandra, Caroline; Davanture, Marlène; Turk, Benjamin E. PLoS ONE, Vol. 5, Issue 11 https://doi.org/10.1371/journal.pone.0013935	journal	November 2010
The Fluorescent Toolbox for Assessing Protein Location and Function Giepmans, B. N. G. Science, Vol. 312, Issue 5771 https://doi.org/10.1126/science.1124618	journal	April 2006
Auxin and ethylene: collaborators or competitors? Muday, Gloria K.; Rahman, Abidur; Binder, Brad M. Trends in Plant Science, Vol. 17, Issue 4 https://doi.org/10.1016/j.tplants.2012.02.001	journal	April 2012
Abscisic acid Biosynthesis and Catabolism Nambara, Eiji; Marion-Poll, Annie Annual Review of Plant Biology, Vol. 56, Issue 1 https://doi.org/10.1146/annurev.arplant.56.032604.144046	journal	June 2005
A Spatio-Temporal Understanding of Growth Regulation during the Salt Stress Response in Arabidopsis Geng, Y.; Wu, R.; Wee, C. W. The Plant Cell, Vol. 25, Issue 6 https://doi.org/10.1105/tpc.113.112896	journal	June 2013
Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo MGG Molecular & General Genetics, Vol. 236-236, Issue 2-3 https://doi.org/10.1007/BF00277130	journal	January 1993
Chemical Genetics Reveals Negative Regulation of Abscisic Acid Signaling by a Plant Immune Response Pathway Kim, Tae-Houn; Hauser, Felix; Ha, Tracy Current Biology, Vol. 21, Issue 11 https://doi.org/10.1016/j.cub.2011.04.045	journal	June 2011
The abscisic acid receptor PYR1 in complex with abscisic acid Santiago, Julia; Dupeux, Florine; Round, Adam Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08591	journal	November 2009
The long‐distance abscisic acid signal in the droughted plant: the fate of the hormone on its way from root to shoot Sauter, Angela; Davies, W. J.; Hartung, Wolfram Journal of Experimental Botany, Vol. 52, Issue 363 https://doi.org/10.1093/jexbot/52.363.1991	journal	October 2001
K+ channels of stomatal guard cells: Abscisic-acid-evoked control of the outward rectifier mediated by cytoplasmic pH Blatt, MichaelR.; Armstrong, Fiona Planta, Vol. 191, Issue 3 https://doi.org/10.1007/BF00195690	journal	August 1993
The role of stomata in sensing and driving environmental change Hetherington, Alistair M.; Woodward, F. Ian Nature, Vol. 424, Issue 6951 https://doi.org/10.1038/nature01843	journal	August 2003
Structural Insights into the Abscisic Acid Stereospecificity by the ABA Receptors PYR/PYL/RCAR Zhang, Xingliang; Jiang, Lun; Wang, Guoqiang PLoS ONE, Vol. 8, Issue 7 https://doi.org/10.1371/journal.pone.0067477	journal	July 2013
In vitro reconstitution of an abscisic acid signalling pathway Fujii, Hiroaki; Chinnusamy, Viswanathan; Rodrigues, Americo Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08599	journal	November 2009
Optogenetic reporters Alford, Spencer C.; Wu, Jiahui; Zhao, Yongxin Biology of the Cell, Vol. 105, Issue 1 https://doi.org/10.1111/boc.201200054	journal	December 2012
A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics Wend, Sabrina; Bosco, Cristina Dal; Kämpf, Michael M. Scientific Reports, Vol. 3, Issue 1 https://doi.org/10.1038/srep02052	journal	June 2013
Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase Sirichandra, Caroline; Gu, Dan; Hu, Heng-Cheng FEBS Letters, Vol. 583, Issue 18 https://doi.org/10.1016/j.febslet.2009.08.033	journal	August 2009
Transport of ABA from the site of biosynthesis to the site of action Seo, Mitsunori; Koshiba, Tomokazu Journal of Plant Research, Vol. 124, Issue 4 https://doi.org/10.1007/s10265-011-0411-4	journal	March 2011
Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta Waadt, Rainer; Schmidt, Lena K.; Lohse, Marc The Plant Journal, Vol. 56, Issue 3 https://doi.org/10.1111/j.1365-313X.2008.03612.x	journal	November 2008
Quantitative Imaging with Fluorescent Biosensors Okumoto, Sakiko; Jones, Alexander; Frommer, Wolf B. Annual Review of Plant Biology, Vol. 63, Issue 1 https://doi.org/10.1146/annurev-arplant-042110-103745	journal	June 2012
A thermodynamic switch modulates abscisic acid receptor sensitivity: A thermodynamic switch in the ABA pathway Dupeux, Florine; Santiago, Julia; Betz, Katja The EMBO Journal, Vol. 30, Issue 20 https://doi.org/10.1038/emboj.2011.294	journal	August 2011
Conformations of variably linked chimeric proteins evaluated by synchrotron X-ray small-angle scattering Arai, Ryoichi; Wriggers, Willy; Nishikawa, Yukihiro Proteins: Structure, Function, and Bioinformatics, Vol. 57, Issue 4 https://doi.org/10.1002/prot.20244	journal	December 2004
PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis: Arabidopsis ABI1 interacting proteins Nishimura, Noriyuki; Sarkeshik, Ali; Nito, Kazumasa The Plant Journal, Vol. 61, Issue 2 https://doi.org/10.1111/j.1365-313X.2009.04054.x	journal	November 2009
Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Ulmasov, T.; Murfett, J.; Hagen, G. The Plant Cell, Vol. 9, Issue 11 https://doi.org/10.1105/tpc.9.11.1963	journal	November 1997
A novel sensor to map auxin response and distribution at high spatio-temporal resolution Brunoud, Géraldine; Wells, Darren M.; Oliva, Marina Nature, Vol. 482, Issue 7383 https://doi.org/10.1038/nature10791	journal	January 2012
Structural insights into the mechanism of abscisic acid signaling by PYL proteins Yin, Ping; Fan, He; Hao, Qi Nature Structural & Molecular Biology, Vol. 16, Issue 12 https://doi.org/10.1038/nsmb.1730	journal	November 2009
PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid Kang, J.; Hwang, J. -U.; Lee, M. Proceedings of the National Academy of Sciences, Vol. 107, Issue 5 https://doi.org/10.1073/pnas.0909222107	journal	January 2010
ABA perception and signalling Raghavendra, Agepati S.; Gonugunta, Vijay K.; Christmann, Alexander Trends in Plant Science, Vol. 15, Issue 7 https://doi.org/10.1016/j.tplants.2010.04.006	journal	July 2010
Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs Santiago, Julia; Rodrigues, Americo; Saez, Angela The Plant Journal, Vol. 60, Issue 4 https://doi.org/10.1111/j.1365-313X.2009.03981.x	journal	August 2009
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Three Arabidopsis SnRK2 Protein Kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, Involved in ABA Signaling are Essential for the Control of Seed Development and Dormancy Nakashima, Kazuo; Fujita, Yasunari; Kanamori, Norihito Plant and Cell Physiology, Vol. 50, Issue 7 https://doi.org/10.1093/pcp/pcp083	journal	June 2009
The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression Norris, Susan R.; Meyer, Sandra E.; Callis, Judy Plant Molecular Biology, Vol. 21, Issue 5 https://doi.org/10.1007/BF00027120	journal	March 1993
Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity Rekas, Agata; Alattia, Jean-René; Nagai, Takeharu Journal of Biological Chemistry, Vol. 277, Issue 52 https://doi.org/10.1074/jbc.M209524200	journal	October 2002
Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors Ma, Y.; Szostkiewicz, I.; Korte, A. Science https://doi.org/10.1126/science.1172408	journal	April 2009
Fiji: an open-source platform for biological-image analysis Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2019	journal	June 2012
Activation of abscisic acid biosynthesis in the leaves of Arabidopsis thaliana in response to water deficit Ikegami, Keiichi; Okamoto, Masanori; Seo, Mitsunori Journal of Plant Research, Vol. 122, Issue 2 https://doi.org/10.1007/s10265-008-0201-9	journal	December 2008
Cytosolic Alkalization and Cytosolic Calcium Oscillation in Arabidopsis Guard Cells Response to ABA and MeJA Islam, M. M.; Hossain, M. A.; Jannat, R. Plant and Cell Physiology, Vol. 51, Issue 10 https://doi.org/10.1093/pcp/pcq131	journal	August 2010
The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh L�ng, Viola; Palva, E. Tapio Plant Molecular Biology, Vol. 20, Issue 5 https://doi.org/10.1007/BF00027165	journal	December 1992
Endodermal ABA Signaling Promotes Lateral Root Quiescence during Salt Stress in Arabidopsis Seedlings Duan, Lina; Dietrich, Daniela; Ng, Chong Han The Plant Cell, Vol. 25, Issue 1 https://doi.org/10.1105/tpc.112.107227	journal	January 2013
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Clough, Steven J.; Bent, Andrew F. The Plant Journal, Vol. 16, Issue 6, p. 735-743 https://doi.org/10.1046/j.1365-313x.1998.00343.x	journal	December 1998
The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells Nagaya, Shingo; Kawamura, Kazue; Shinmyo, Atsuhiko Plant and Cell Physiology, Vol. 51, Issue 2 https://doi.org/10.1093/pcp/pcp188	journal	December 2009
Drought Induction of Arabidopsis 9-cis-Epoxycarotenoid Dioxygenase Occurs in Vascular Parenchyma Cells Endo, Akira; Sawada, Yoshiaki; Takahashi, Hirokazu Plant Physiology, Vol. 147, Issue 4 https://doi.org/10.1104/pp.108.116632	journal	June 2008
Rapid Adjustment of Guard-Cell Abscisic Acid Levels to Current Leaf-Water Status Harris, Michael J.; Outlaw, William H. Plant Physiology, Vol. 95, Issue 1 https://doi.org/10.1104/pp.95.1.171	journal	January 1991
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation Walter, Michael; Chaban, Christina; Schütze, Katia The Plant Journal, Vol. 40, Issue 3 https://doi.org/10.1111/j.1365-313X.2004.02219.x	journal	September 2004
Guard Cell Signal Transduction Network: Advances in Understanding Abscisic Acid, CO ₂ , and Ca ²⁺ Signaling Kim, Tae-Houn; Böhmer, Maik; Hu, Honghong Annual Review of Plant Biology, Vol. 61, Issue 1 https://doi.org/10.1146/annurev-arplant-042809-112226	journal	June 2010
Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance Okamoto, M.; Peterson, F. C.; Defries, A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 29 https://doi.org/10.1073/pnas.1305919110	journal	July 2013
The Stomatal Response to Reduced Relative Humidity Requires Guard Cell-Autonomous ABA Synthesis Bauer, Hubert; Ache, Peter; Lautner, Silke Current Biology, Vol. 23, Issue 1 https://doi.org/10.1016/j.cub.2012.11.022	journal	January 2013
Protein Fragment Bimolecular Fluorescence Complementation Analyses for the In vivo Study of Protein-Protein Interactions and Cellular Protein Complex Localizations Waadt, Rainer; Schlücking, Kathrin; Schroeder, Julian I. Arabidopsis Protocols https://doi.org/10.1007/978-1-62703-580-4_33	book	August 2013
Genetic analysis of osmotic and cold stress signal transduction in Arabidopsis: interactions and convergence of abscisic acid-dependent and abscisic acid-independent pathways. Ishitani, M.; Xiong, L.; Stevenson, B. The Plant Cell, Vol. 9, Issue 11 https://doi.org/10.1105/tpc.9.11.1935	journal	November 1997
Abscisic acid dynamics in roots detected with genetically encoded FRET sensors Jones, Alexander M.; Danielson, Jonas ÅH; ManojKumar, Shruti N. eLife, Vol. 3 https://doi.org/10.7554/eLife.01741	journal	April 2014
Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins Nagai, T.; Yamada, S.; Tominaga, T. Proceedings of the National Academy of Sciences, Vol. 101, Issue 29 https://doi.org/10.1073/pnas.0400417101	journal	July 2004
Different Plant Hormones Regulate Similar Processes through Largely Nonoverlapping Transcriptional Responses Nemhauser, Jennifer L.; Hong, Fangxin; Chory, Joanne Cell, Vol. 126, Issue 3 https://doi.org/10.1016/j.cell.2006.05.050	journal	August 2006
Arabidopsis mutants of AtABCG22, an ABC transporter gene, increase water transpiration and drought susceptibility: AtABCG22 mutants increase water transpiration Kuromori, Takashi; Sugimoto, Eriko; Shinozaki, Kazuo The Plant Journal, Vol. 67, Issue 5 https://doi.org/10.1111/j.1365-313X.2011.04641.x	journal	June 2011
Localized changes in apoplastic and cytoplasmic pH are associated with root hair development in Arabidopsis thaliana Bibikova, T. N.; Jacob, T.; Dahse, I. Development, Vol. 125, Issue 15 https://doi.org/10.1242/dev.125.15.2925	journal	August 1998
Abscisic acid-activated SNRK2 protein kinases function in the gene-regulation pathway of ABA signal transduction by phosphorylating ABA response element-binding factors: TRAB1 phosphorylation by SnRK2 protein kinases Kobayashi, Yuhko; Murata, Michiharu; Minami, Hideyuki The Plant Journal, Vol. 44, Issue 6 https://doi.org/10.1111/j.1365-313X.2005.02583.x	journal	November 2005
Rapid Development of Genetically Encoded FRET Reporters Piljić, Alen; de Diego, Iñaki; Wilmanns, Matthias ACS Chemical Biology, Vol. 6, Issue 7 https://doi.org/10.1021/cb100402n	journal	April 2011
Auxin: A Trigger for Change in Plant Development Vanneste, Steffen; Friml, Jiří Cell, Vol. 136, Issue 6 https://doi.org/10.1016/j.cell.2009.03.001	journal	March 2009
ABA transport and transporters Boursiac, Yann; Léran, Sophie; Corratgé-Faillie, Claire Trends in Plant Science, Vol. 18, Issue 6 https://doi.org/10.1016/j.tplants.2013.01.007	journal	June 2013
Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1 Furihata, T.; Maruyama, K.; Fujita, Y. Proceedings of the National Academy of Sciences, Vol. 103, Issue 6 https://doi.org/10.1073/pnas.0505667103	journal	January 2006
Potent and selective activation of abscisic acid receptors in vivo by mutational stabilization of their agonist-bound conformation Mosquna, A.; Peterson, F. C.; Park, S. -Y. Proceedings of the National Academy of Sciences, Vol. 108, Issue 51 https://doi.org/10.1073/pnas.1112838108	journal	December 2011
ABA-based chemical signalling: the co-ordination of responses to stress in plants Wilkinson, S.; Davies, W. J. Plant, Cell and Environment, Vol. 25, Issue 2 https://doi.org/10.1046/j.0016-8025.2001.00824.x	journal	February 2002
Bright cyan fluorescent protein variants identified by fluorescence lifetime screening Goedhart, Joachim; van Weeren, Laura; Hink, Mark A. Nature Methods, Vol. 7, Issue 2 https://doi.org/10.1038/nmeth.1415	journal	January 2010
Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor Kanno, Y.; Hanada, A.; Chiba, Y. Proceedings of the National Academy of Sciences, Vol. 109, Issue 24 https://doi.org/10.1073/pnas.1203567109	journal	May 2012
Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABI1 PP2C phosphatase action Brandt, B.; Brodsky, D. E.; Xue, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 26 https://doi.org/10.1073/pnas.1116590109	journal	June 2012
A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells Lee, Sung Chul; Lan, Wenzhi; Buchanan, Bob B. Proceedings of the National Academy of Sciences, Vol. 106, Issue 50 https://doi.org/10.1073/pnas.0910601106	journal	December 2009
Ca2+ Indicators Based on Computationally Redesigned Calmodulin-Peptide Pairs Palmer, Amy E.; Giacomello, Marta; Kortemme, Tanja Chemistry & Biology, Vol. 13, Issue 5 https://doi.org/10.1016/j.chembiol.2006.03.007	journal	May 2006

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