Construction of an in vitro primary lung co-culture platform derived from New Zealand white rabbits

Journal Article · Fri May 01 00:00:00 EDT 2015 · In Vitro Cellular & Developmental Biology – Animal, 51(5):433-440

DOI:https://doi.org/10.1007/s11626-014-9853-z· OSTI ID:1191805

Powell, Joshua D.; Hess, Becky M.; Hutchison, Janine R.; Straub, Tim M.

We report the construction of an in vitro three dimensional (3D) co-culture platform consisting of differentiated lung epithelial cells and monocytes from New Zealand white rabbits. Rabbit lung epithelial cells were successfully grown at air-liquid interface, produced mucus, and expressed both sialic acid alpha-2,3 and alpha-2,6. Blood-derived CD14+ monocytes were deposited above the epithelial layer resulting in the differentiation of a subset of monocytes into CD11c+ cells within the co-culture. These proof-of-concept findings provide a convenient means to comparatively study in vitro versus in vivo rabbit lung responses as they relate to inhalation or lung-challenge studies.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1191805
• Report Number(s): PNNL-SA-104791; 400904120
• Journal Information: In Vitro Cellular & Developmental Biology – Animal, 51(5):433-440, Journal Name: In Vitro Cellular & Developmental Biology – Animal, 51(5):433-440
• Country of Publication: United States
• Language: English

Related Subjects

rabbit
lung
primary epithelial