Streptomyces coelicolor SCO4226 Is a Nickel Binding Protein

Lu, Mo; Jiang, Yong-Liang; Wang, Shu; Jin, Hua; Zhang, Rong-Guang; Virolle, Marie-Joelle; Chen, Yuxing; Zhou, Cong-Zhao

doi:10.1371/journal.pone.0109660

Title: Streptomyces coelicolor SCO4226 Is a Nickel Binding Protein

Journal Article · Mon Oct 06 00:00:00 EDT 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0109660· OSTI ID:1191713

Lu, Mo ^[1]; Jiang, Yong-Liang ^[1]; Wang, Shu ^[1]; Jin, Hua ^[1]; Zhang, Rong-Guang ^[2]; Virolle, Marie-Joelle ^[3]; Chen, Yuxing ^[1]; Zhou, Cong-Zhao ^[1]

Univ. of Science and Technology of China, Hefei (China)
Chinese Academy of Sciences (CAS), Beijing (China)
Univ. Paris-Sud, Orsay (France)

The open reading frame SCO4226 of Streptomyces coelicolor A3(2) encodes an 82-residue hypothetical protein. Biochemical assays revealed that each SCO4226 dimer binds four nickel ions. To decipher the molecular function, we solved the crystal structures of SCO4226 in both apo- and nickel-bound (Ni-SCO4226) forms at 1.30 and 2.04 Å resolution, respectively. Each subunit of SCO4226 dimer adopts a canonical ferredoxin-like fold with five β-strands flanked by two α-helices. In the structure of Ni-SCO4226, four nickel ions are coordinated at the surface of the dimer. Further biochemical assays suggested that the binding of Ni2+ triggers the self-aggregation of SCO4226 in vitro. In addition, RT-qPCR assays demonstrated that the expression of SCO4226 gene in S. coelicolor is specifically up-regulated by the addition of Ni²⁺, but not other divalent ions such as Cu²⁺, Mn²⁺ or Co²⁺. All these results suggested that SCO4226 acts as a nickel binding protein, probably required for nickel sequestration and/or detoxification.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

OSTI ID:: 1191713

Journal Information:: PLoS ONE, Vol. 9, Issue 10; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 9 works

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