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Title: Characterization and use of the spent beam for serial operation of LCLS

Journal Article · · Journal of Synchrotron Radiation (Online)

X-ray free-electron laser sources such as the Linac Coherent Light Source offer very exciting possibilities for unique research. However, beam time at such facilities is very limited and in high demand. This has led to significant efforts towards beam multiplexing of various forms. One such effort involves re-using the so-called spent beam that passes through the hole in an area detector after a weak interaction with a primary sample. This beam can be refocused into a secondary interaction region and used for a second, independent experiment operating in series. The beam profile of this refocused beam was characterized for a particular experimental geometry at the Coherent X-ray Imaging instrument at LCLS. A demonstration of this multiplexing capability was performed with two simultaneous serial femtosecond crystallography experiments, both yielding interpretable data of sufficient quality to produce electron density maps.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
OSTI ID:
1189939
Journal Information:
Journal of Synchrotron Radiation (Online), Vol. 22, Issue 3; ISSN 1600-5775
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

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Cited By (10)

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Multi-wavelength anomalous diffraction de novo phasing using a two-colour X-ray free-electron laser with wide tunability journal October 2017
Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin journal April 2017
An outlook on using serial femtosecond crystallography in drug discovery journal May 2019
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation journal April 2019
In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells journal January 2016
Serial Femtosecond Crystallography text January 2015
Climbing the Data Mountain: Processing of SFX Data text January 2018
The Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation text January 2019
Serial Femtosecond Crystallography journal November 2015