Bioaccumulation of dietary 2,2{prime},4,4{prime},5,5{prime}-hexachlorobiphenyl and induction of hepatic arylhydrocarbon hydroxylase in rainbow trout (Oncorhynchus mykiss)
- Oregon State Univ., Corvallis, OR (United States). Oak Creek Lab. of Biology
Juvenile rainbow trout (Oncorhynchus mykiss) were fed either 5 or 20 {micro}g, 2,2{prime},4,4{prime},5,5{prime}-hexachlorobiphenyl (245-HxCB)/g diet (wet wt.) for 4,8, or 12 weeks. Hepatic xenobiotic-metabolizing enzyme activities and dietary 245-HxCB accumulation in liver, muscle, and remaining carcass were determined. Liver-to-body weight ratios were not altered by either of the two 245-HxCB concentrations. Relative growth rate increased with time but was not altered by 245-HxCB concentration. Bioaccumulation of 245-HxCB was dose and time dependent in all tissues without reaching apparent stayed state. Hepatic arylhydrocarbon hydroxylase (AHH) activities increased with 245-HxCB dose and with time. Ethoxyresorufin-O-deethylase (EROD) activities also increased in fish fed 20 {micro}g 245-HxCB/g diet. No 245-HxCB-induced changes in uridine diphosphoglucuronosyl transferase (UDP-DT) or NADPH-dytochrome-c reductase (NCCR) activities were determined. High-resolution GC-MS analysis of the 245-HxCB standard revealed trace (0.4--0.5%) contamination by two mono-ortho pentachlorobiphenyls (PnCBs): 2,3,3{prime},4,4{prime}-PnCB and 2,3,4,4{prime},5-PnCB. Total liver accumulation of these contaminants was inversely related with corresponding EROD and AHH activities and estimated to contribute minimally to their induction. Results from this study suggested that long-term dietary 245-HxCB exposure induced cytochrome P4501A activities in rainbow trout liver.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 118642
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 14, Issue 10; Other Information: PBD: Oct 1995
- Country of Publication:
- United States
- Language:
- English
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