U.S. Department of EnergyOffice of Scientific and Technical Information
Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation
Abstract
The alternative sigma factor E (σE) is critical for response to extracytoplasmic stress in Salmonella. Extensive studies have been conducted on σE-regulated gene expression, particularly at the transcriptional level. Increasing evidence suggests however that σE may indirectly participate in post-transcriptional regulation. Here in this study, we conducted sample-matched global proteomic and transcriptomic analyses to determine the level of regulation mediated by σE in Salmonella. We analysed samples from wild type and isogenic rpoE mutant Salmonella cultivated in three different conditions; nutrient-rich and conditions that mimic early and late intracellular infection. We found that 30% of the observed proteome was regulated by σE combining all three conditions. In different growth conditions, σE affected the expression of a broad spectrum of Salmonella proteins required for miscellaneous functions. Those involved in transport and binding, protein synthesis, and stress response were particularly highlighted. By comparing transcriptomic and proteomic data, we identified genes post-transcriptionally regulated by σE and found that post-transcriptional regulation was responsible for a majority of changes observed in the σE-regulated proteome. Further, comparison of transcriptomic and proteomic data from hfq mutant of Salmonella demonstrated that σE–mediated post-transcriptional regulation was partially dependent on the RNA-binding protein Hfq.
- Authors:
-
- Oregon Health & Science Univ., Portland, OR (United States). Dept. of Molecular Microbiology and Immunology
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH)
- OSTI Identifier:
- 1182899
- Report Number(s):
- PNNL-SA-105707
Journal ID: ISSN 1535-3893; 42294; 47623; 48135; 47418; 400412000
- Grant/Contract Number:
- AC05-76RLO01830; GM094623; GM103493
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Journal of Proteome Research
- Additional Journal Information:
- Journal Volume: 14; Journal Issue: 4; Journal ID: ISSN 1535-3893
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; infection; post-transcriptional regulation; proteomics; Salmonella; sigma factor E; transcriptomics; virulence
Citation Formats
Li, Jie, Nakayasu, Ernesto S., Overall, Christopher C., Johnson, Rudd C., Kidwai, Afshan S., McDermott, Jason E., Ansong, Charles, Heffron, Fred, Cambronne, Eric D., and Adkins, Joshua N. Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation. United States: N. p., 2015.
Web. doi:10.1021/pr5010423.
Li, Jie, Nakayasu, Ernesto S., Overall, Christopher C., Johnson, Rudd C., Kidwai, Afshan S., McDermott, Jason E., Ansong, Charles, Heffron, Fred, Cambronne, Eric D., & Adkins, Joshua N. Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation. United States. https://doi.org/10.1021/pr5010423
Li, Jie, Nakayasu, Ernesto S., Overall, Christopher C., Johnson, Rudd C., Kidwai, Afshan S., McDermott, Jason E., Ansong, Charles, Heffron, Fred, Cambronne, Eric D., and Adkins, Joshua N. 2015.
"Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation". United States. https://doi.org/10.1021/pr5010423. https://www.osti.gov/servlets/purl/1182899.
@article{osti_1182899,
title = {Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation},
author = {Li, Jie and Nakayasu, Ernesto S. and Overall, Christopher C. and Johnson, Rudd C. and Kidwai, Afshan S. and McDermott, Jason E. and Ansong, Charles and Heffron, Fred and Cambronne, Eric D. and Adkins, Joshua N.},
abstractNote = {The alternative sigma factor E (σE) is critical for response to extracytoplasmic stress in Salmonella. Extensive studies have been conducted on σE-regulated gene expression, particularly at the transcriptional level. Increasing evidence suggests however that σE may indirectly participate in post-transcriptional regulation. Here in this study, we conducted sample-matched global proteomic and transcriptomic analyses to determine the level of regulation mediated by σE in Salmonella. We analysed samples from wild type and isogenic rpoE mutant Salmonella cultivated in three different conditions; nutrient-rich and conditions that mimic early and late intracellular infection. We found that 30% of the observed proteome was regulated by σE combining all three conditions. In different growth conditions, σE affected the expression of a broad spectrum of Salmonella proteins required for miscellaneous functions. Those involved in transport and binding, protein synthesis, and stress response were particularly highlighted. By comparing transcriptomic and proteomic data, we identified genes post-transcriptionally regulated by σE and found that post-transcriptional regulation was responsible for a majority of changes observed in the σE-regulated proteome. Further, comparison of transcriptomic and proteomic data from hfq mutant of Salmonella demonstrated that σE–mediated post-transcriptional regulation was partially dependent on the RNA-binding protein Hfq.},
doi = {10.1021/pr5010423},
url = {https://www.osti.gov/biblio/1182899},
journal = {Journal of Proteome Research},
issn = {1535-3893},
number = 4,
volume = 14,
place = {United States},
year = {Mon Feb 16 00:00:00 EST 2015},
month = {Mon Feb 16 00:00:00 EST 2015}
}
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