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Title: Methods and materials for deconstruction of biomass for biofuels production

Patent ·
OSTI ID:1179022

The present invention relates to nucleic acids, peptides, vectors, cells, and plants useful in the production of biofuels. In certain embodiments, the invention relates to nucleic acid sequences and peptides from extremophile organisms, such as SSO1949 and Ce1A, that are useful for hydrolyzing plant cell wall materials. In further embodiments, the invention relates to modified versions of such sequences that have been optimized for production in one or both of monocot and dicot plants. In other embodiments, the invention provides for targeting peptide production or activity to a certain location within the cell or organism, such as the apoplast. In further embodiments, the invention relates to transformed cells or plants. In additional embodiments, the invention relates to methods of producing biofuel utilizing such nucleic acids, peptides, targeting sequences, vectors, cells, and/or plants.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC04-94AL85000
Assignee:
Sandia Corporation (Albuquerque, NM)
Patent Number(s):
9,024,111
Application Number:
13/436,508
OSTI ID:
1179022
Resource Relation:
Patent File Date: 2012 Mar 30
Country of Publication:
United States
Language:
English

References (7)

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Simple and versatile selection of Arabidopsis transformants journal August 2002
A highly acid-stable and thermostable endo-β-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus journal January 2005
Global potential bioethanol production from wasted crops and crop residues journal April 2004
Dramatic effects of truncation and sub-cellular targeting on the accumulation of recombinant microbial cellulase in tobacco journal September 2001
Microbial &bgr;-glucuronidase genes, gene products and uses thereof patent May 2002
Glucanases, Nucleic Acids Encoding Them And Methods For Making And Using Them patent-application September 2008