Application of an Efficient Gene Targeting System Linking Secondary Metabolites to their Biosynthetic Genes in Aspergillus terreus
Journal Article
·
· Organic Letters, 15(14):3562-3565
Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillusterreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP related melanin synthetase was also identified in this species.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1177332
- Report Number(s):
- PNNL-SA-95505; BM0101020
- Journal Information:
- Organic Letters, 15(14):3562-3565, Journal Name: Organic Letters, 15(14):3562-3565
- Country of Publication:
- United States
- Language:
- English
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