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Title: Disruption of cell walls for enhanced lipid recovery

Patent ·
OSTI ID:1174202
; ; ; ;

Presented herein are methods of using cell wall degrading enzymes for recovery of internal lipid bodies from biomass sources such as algae. Also provided are algal cells that express at least one exogenous gene encoding a cell wall degrading enzyme and methods for recovering lipids from the cells.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
8,986,977
Application Number:
13/731,578
OSTI ID:
1174202
Resource Relation:
Patent File Date: 2012 Dec 31
Country of Publication:
United States
Language:
English

References (14)

Microorganisms and methods for degrading plant cell walls and complex hydrocarbons patent September 1991
Cell-wall degrading enzyme variants patent August 2003
Isolation of microbial oils patent October 2008
Composition comprising enzymatically digested yeast cells and method of preparing same patent January 2013
Bacterial galactanases and use thereof patent-application January 2003
Commercial production of polysaccharide degrading enzymes in plants and methods of using same patent-application February 2006
Method for Processing an Algae Medium Containing Algae Microorganisms to Produce Algal Oil and By-Products patent-application June 2010
Process of Producing Oil from Algae Using Biological Rupturing patent-application December 2010
Methods of saccharification of polysaccharides in plants patent-application April 2011
Analysis of 76 kb of the Chlorella Virus PBCV-1 330-kb Genome: Map Positions 182 to 258 journal September 1996
Accurate and reliable quantification of total microalgal fuel potential as fatty acid methyl esters by in situ transesterification journal February 2012
Analysis of 74 kb of DNA Located at the Right End of the 330-kb Chlorella Virus PBCV-1 Genome journal October 1997
Analysis of 94 kb of the Chlorella Virus PBCV-1 330-kb Genome: Map Positions 88 to 182 journal February 1996
Characterization of a β-1,3-Glucanase Encoded by Chlorella Virus PBCV-1 journal October 2000

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