Characterization of protein redox dynamics induced during light-to-dark transitions and nutrient limitation in cyanobacteria
Protein redox chemistry constitutes a major void in knowledge pertaining to photoautotrophic system regulation and signaling processes. We have employed a chemical biology approach to analyze redox sensitive proteins in live Synechococcus sp. PCC 7002 cells in both light and dark periods, and to understand how cellular redox balance is disrupted during nutrient perturbation. The present work identified several novel putative redox-sensitive proteins that are involved in the generation of reductant, macromolecule synthesis, and carbon flux through central metabolic pathways, and may be involved in cell signaling and response mechanisms. Furthermore, our research suggests that dynamic redox changes in response to specific nutrient limitations contribute to the regulatory changes driven by a shift from light to dark. Taken together, these results contribute to the high-level understanding of post-translational mechanisms regulating flux distributions and therefore present potential metabolic engineering targets for redirecting carbon towards biofuel precursors.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1171873
- Report Number(s):
- PNNL-SA-101966; 47297; KP1601010
- Journal Information:
- Frontiers in Microbiology, 5:Article No. 325, Journal Name: Frontiers in Microbiology, 5:Article No. 325
- Country of Publication:
- United States
- Language:
- English
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