Study of polymer molecules and conformations with a nanopore

Golovchenko, Jene A.; Li, Jiali; Stein, Derek; Gershow, Marc H.

Title: Study of polymer molecules and conformations with a nanopore

Patent · Tue Mar 03 00:00:00 EST 2015

OSTI ID:1171692

Golovchenko, Jene A.; Li, Jiali; Stein, Derek; Gershow, Marc H.

The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.

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Research Organization:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-01ER45922

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: 8,969,091

Application Number:: 13/766,231

OSTI ID:: 1171692

Resource Relation:: Patent File Date: 2013 Feb 13

Country of Publication:: United States

Language:: English

References (114)

Ion-beam sculpting at nanometre length scales Li, Jiali; Stein, Derek; McMullan, Ciaran Nature, Vol. 412, Issue 6843, p. 166-169 https://doi.org/10.1038/35084037	journal	July 2001
Alignment and sensitive detection of DNA by a moving interface Bensimon, A.; Simon, A.; Chiffaudel, A. Science, Vol. 265, Issue 5181 https://doi.org/10.1126/science.7522347	journal	September 1994
Apparatus for studying particles having an aperture whose cross-sectional area changes progressively along at least part of its length Doutre, Don https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4926114 US Patent Document 4,926,114	patent	May 1990
Electrical biosensor containing a biological receptor immobilized and stabilized in a protein film Taylor, Richard Francis; Marenchic, Ingrid G.; Cook, Edward J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5001048 US Patent Document 5,001,048	patent	March 1991
Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore Movileanu, Liviu; Howorka, Stefan; Braha, Orit Nature Biotechnology, Vol. 18, Issue 10 https://doi.org/10.1038/80295	journal	October 2000
Methods and apparatus for characterizing polynucleotides Akeson, Mark A.; Branton, Daniel; Deamer, David W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7238485 US Patent Document 7,238,485	patent	July 2007
DNA molecules and configurations in a solid-state nanopore microscope Li, Jiali; Gershow, Marc; Stein, Derek Nature Materials, Vol. 2, Issue 9 https://doi.org/10.1038/nmat965	journal	August 2003
Alignment of Conduits for the Nascent Polypeptide Chain in the Ribosome-Sec61 Complex Beckmann, R. Science, Vol. 278, Issue 5346 https://doi.org/10.1126/science.278.5346.2123	journal	December 1997
Process and device for determination of parameters of particles in electrolytes Preiss, Willi; Beckmann, Dieter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5911871 US Patent Document 5,911,871	patent	June 1999
Single-channel currents recorded from membrane of denervated frog muscle fibres Neher, Erwin; Sakmann, Bert Nature, Vol. 260, Issue 5554 https://doi.org/10.1038/260799a0	journal	April 1976
The voltage-dependent activity of Escherichia coli porins in different planar bilayer reconstitutions Lakey, Jeremy H.; Pattus, Franc European Journal of Biochemistry, Vol. 186, Issue 1-2 https://doi.org/10.1111/j.1432-1033.1989.tb15209.x	journal	December 1989
Nucleic acid sequencing using an optically labeled pore Russell, Terence https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6528258 US Patent Document 6,528,258	patent	March 2003
Characterization of Nucleic Acids by Nanopore Analysis Deamer, David W.; Branton, Daniel Accounts of Chemical Research, Vol. 35, Issue 10 https://doi.org/10.1021/ar000138m	journal	October 2002
Induction of DNA replication by transcription in the region upstream of the human c-myc gene in a model replication system. Ohba, R.; Matsumoto, K.; Ishimi, Y. Molecular and Cellular Biology, Vol. 16, Issue 10 https://doi.org/10.1128/MCB.16.10.5754	journal	October 1996
Single-molecule analysis of DNA-protein complexes using nanopores Hornblower, Breton; Coombs, Amy; Whitaker, Richard D. Nature Methods, Vol. 4, Issue 4 https://doi.org/10.1038/nmeth1021	journal	March 2007
Support and anchoring mechanism for membranes in selectively responsive field effect devices Blackburn, Gary https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4456522 US Patent Document 4,456,522	patent	June 1984
Methods and products for analyzing polymers Chan, Eugene Y. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020119455 US Patent Application 09/852968; 20020119455	patent-application	August 2002
Stochastic sensors inspired by biology Bayley, Hagan; Cremer, Paul S. Nature, Vol. 413, Issue 6852, p. 226-230 https://doi.org/10.1038/35093038	journal	September 2001
Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel Vercoutere, Wenonah; Winters-Hilt, Stephen; Olsen, Hugh Nature Biotechnology, Vol. 19, Issue 3 https://doi.org/10.1038/85696	journal	March 2001
Methods and apparatus for characterizing polynucleotides Akeson, Mark A.; Branton, Daniel; Deamer, David W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7947454 US Patent Document 7,947,454	patent	May 2011
Artificial transmembrane ion channels from self-assembling peptide nanotubes Ghadiri, M. Reza; Granja, Juan R.; Buehler, Lukas K. Nature, Vol. 369, Issue 6478 https://doi.org/10.1038/369301a0	journal	May 1994
Method and apparatus for reducing the distortion of a sample zone eluting from a capillary electrophoresis capillary Nordman, Eric S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5833826 US Patent Document 5,833,826	patent	November 1998
Genetic analysis of membrane protein topology by a sandwich gene fusion approach. Ehrmann, M.; Boyd, D.; Beckwith, J. Proceedings of the National Academy of Sciences, Vol. 87, Issue 19 https://doi.org/10.1073/pnas.87.19.7574	journal	October 1990
Methods of determining the presence of double stranded nucleic acids in a sample Deamer, David W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6617113 US Patent Document 6,617,113	patent	September 2003
Open channel noise. V. Fluctuating barriers to ion entry in gramicidin A channels Heinemann, S. H.; Sigworth, F. J. Biophysical Journal, Vol. 57, Issue 3, p. 499-514 https://doi.org/10.1016/S0006-3495(90)82566-2	journal	March 1990
Mechanism of sugar transport through the sugar-specific LamB channel ofEscherichia coli outer membrane Benz, Roland; Schmid, Angela; Vos-Scheperkeuter, Greetje H. The Journal of Membrane Biology, Vol. 100, Issue 1 https://doi.org/10.1007/BF02209137	journal	December 1987
Optimizing planar lipid bilayer single-channel recordings for high resolution with rapid voltage steps Wonderlin, W. F.; Finkel, A.; French, R. J. Biophysical Journal, Vol. 58, Issue 2, p. 289-297 https://doi.org/10.1016/S0006-3495(90)82376-6	journal	August 1990
Sensitivity and selectivity of ion channel biosensor membranes Osman, Peter D.; Cornell, Bruce A.; Raguse, Burkhard https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5234566 US Patent Document 5,234,566	patent	August 1993
Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12 Boulain, Jean Claude; Charbit, Alain; Hofnung, Maurice MGG Molecular & General Genetics, Vol. 205, Issue 2 https://doi.org/10.1007/BF00430448	journal	November 1986
Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymerase Ollis, D. L.; Kline, C.; Steitz, T. A. Nature, Vol. 313, Issue 6005 https://doi.org/10.1038/313818a0	journal	February 1985
Molecular and atomic scale evaluation of biopolymers Branton, Daniel; Golovchenko, Jene A.; Denison, Timothy J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6627067 US Patent Document 6,627,067	patent	September 2003
A protein-conducting channel in the endoplasmic reticulum Simon, Sanford M.; Blobel, Günter Cell, Vol. 65, Issue 3, p. 371-380 https://doi.org/10.1016/0092-8674(91)90455-8	journal	May 1991
Sequencing and the Single Channel Andersen, Olaf Sparre Biophysical Journal, Vol. 77, Issue 6, p. 2899-2901 https://doi.org/10.1016/S0006-3495(99)77122-5	journal	December 1999
Method for characterizing nucleic acid molecules Jendrisak, Jerome; Hoffman, Leslie M.; Smith, III, Robert E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6190865 US Patent Document 6,190,865	patent	February 2001
Synthetic nanopores for molecular spectroscopy Tabib-Azar, Massood; Moetakef, Pouya; Sharghi-Moshtaghin, Reza 2008 IEEE Sensors https://doi.org/10.1109/ICSENS.2008.4716502	conference	October 2008
Instrument for measuring the deforming capacity of red blood corpuscles Kiesewetter, Holger; Myrenne, Heinz; Roggenkamp, Hans-Gunther https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4521729 US Patent Document 4,521,729	patent	June 1985
Methods and apparatus for characterizing polynucleotides Akeson, Mark A.; Branton, Daniel; Deamer, David W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7625706 US Patent Document 7,625,706	patent	December 2009
Open channel noise. IV. Estimation of rapid kinetics of formamide block in gramicidin A channels Heinemann, S. H.; Sigworth, F. J. Biophysical Journal, Vol. 54, Issue 4, p. 757-764 https://doi.org/10.1016/S0006-3495(88)83013-3	journal	October 1988
Molecular motors Chan, Eugene Y. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6210896 US Patent Document 6,210,896	patent	April 2001
Nanopores and nucleic acids: prospects for ultrarapid sequencing Deamer, David W.; Akeson, Mark Trends in Biotechnology, Vol. 18, Issue 4, p. 147-151 https://doi.org/10.1016/S0167-7799(00)01426-8	journal	April 2000
Unzipping Kinetics of Double-Stranded DNA in a Nanopore Sauer-Budge, Alexis F.; Nyamwanda, Jacqueline A.; Lubensky, David K. Physical Review Letters, Vol. 90, Issue 23 https://doi.org/10.1103/PhysRevLett.90.238101	journal	June 2003
A common receptor protein for phage T5 and colicin M in the outer membrane of Escherichia coli B Braun, Volkmar; Schaller, Klaus; Wolff, Helga Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 323, Issue 1, p. 87-97 https://doi.org/10.1016/0005-2736(73)90433-1	journal	September 1973
Effect of point mutations on the in-vitro pore properties of maltoporin, a protein of Escherichia coli outer membrane Dargent, Bénédicte; Charbit, Alain; Hofnung, Maurice Journal of Molecular Biology, Vol. 201, Issue 3, p. 497-506 https://doi.org/10.1016/0022-2836(88)90632-8	journal	June 1988
Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA Molecules Akeson, Mark; Branton, Daniel; Kasianowicz, John J. Biophysical Journal, Vol. 77, Issue 6, p. 3227-3233 https://doi.org/10.1016/S0006-3495(99)77153-5	journal	December 1999
Two types of inactivation in Shaker K⁺ channels: Effects of alterations in the carboxy-terminal region Hoshi, Toshinori; Zagotta, William N.; Aldrich, Richard W. Neuron, Vol. 7, Issue 4, p. 547-556 https://doi.org/10.1016/0896-6273(91)90367-9	journal	October 1991
Molecular architecture and electrostatic properties of a bacterial porin Weiss, M.; Abele, U.; Weckesser, J. Science, Vol. 254, Issue 5038 https://doi.org/10.1126/science.1721242	journal	December 1991
Sandwiched Graphene−Membrane Superstructures Titov, Alexey V.; Král, Petr; Pearson, Ryan ACS Nano, Vol. 4, Issue 1 https://doi.org/10.1021/nn9015778	journal	December 2009
Improved stability of black lipid membranes by coating with polysaccharide derivatives bearing hydrophobic anchor groups Moellerfeld, J.; Prass, W.; Ringsdorf, H. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 857, Issue 2, p. 265-270 https://doi.org/10.1016/0005-2736(86)90355-X	journal	May 1986
Alamethicin. A rich model for channel behavior Hall, J. E.; Vodyanoy, I.; Balasubramanian, T. M. Biophysical Journal, Vol. 45, Issue 1, p. 233-247 https://doi.org/10.1016/S0006-3495(84)84151-X	journal	January 1984
Characterization of individual polymer molecules based on monomer-interface interactions Church, George M.; Deamer, David W.; Branton, Daniel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5795782 US Patent Document 5,795,782	patent	August 1998
Blockade of a mitochondrial cationic channel by an addressing peptide: An electrophysiological study Henry, Jean-Pierre; Chich, Jean-François; Goldschmidt, Denise The Journal of Membrane Biology, Vol. 112, Issue 2 https://doi.org/10.1007/BF01871275	journal	December 1989
Chemo-receptive lipid based membrane transducers Krull, Ulrich J.; Thompson, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4661235 US Patent Document 4,661,235	patent	April 1987
Polymorphism analysis by nucleic acid structure probing Dong, Fang; Lyamichev, Victor; Prudent, James R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6214545 US Patent Document 6,214,545	patent	April 2001
Transcription by T7 RNA polymerase using benzo[a]pyrene-modified templates Nath, Surend T.; Romana, Louis J. Carcinogenesis, Vol. 12, Issue 6 https://doi.org/10.1093/carcin/12.6.973	journal	January 1991
DNA sample preparation and electrophoresis analysis apparatus Kambara, Hideki https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6054035 US Patent Document 6,054,035	patent	April 2000
Voltage-Driven DNA Translocations through a Nanopore Meller, Amit; Nivon, Lucas; Branton, Daniel Physical Review Letters, Vol. 86, Issue 15 https://doi.org/10.1103/PhysRevLett.86.3435	journal	April 2001
RNA polymerase-dependent mechanism for the stepwise T7 phage DNA transport from the virion into E. coli Zavriev, S. K.; Shemyakin, M. F. Nucleic Acids Research, Vol. 10, Issue 5 https://doi.org/10.1093/nar/10.5.1635	journal	January 1982
Biological oscillating device Ikematsu, Mineo; Sugiyama, Yukihiro; Iseki, Masahiro https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5503744 US Patent Document 5,503,744	patent	April 1996
Fluorescence measurement of the kinetics of DNA injection by bacteriophage .lambda. into liposomes Novick, Steven L.; Baldeschwieler, John D. Biochemistry, Vol. 27, Issue 20 https://doi.org/10.1021/bi00420a050	journal	October 1988
"Reversed" alamethicin conductance in lipid bilayers Taylor, R. J.; de Levie, R. Biophysical Journal, Vol. 59, Issue 4, p. 873-879 https://doi.org/10.1016/S0006-3495(91)82299-8	journal	April 1991
Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer Gilmanshin, Rudolf; Chan, Eugene Y. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6263286 US Patent Document 6,263,286	patent	July 2001
Coherent Electron Junction Scanning Probe Interference Microscope, Nanomanipulator and Spectrometer with Assembler and DNA Sequencing Applications Zorn, Miguel Delmar https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070194225 US Patent Application 11/246665; 20070194225	patent-application	August 2007
Nanopores with a spark for single-molecule detection Wang, Hui; Branton, Daniel Nature Biotechnology, Vol. 19, Issue 7 https://doi.org/10.1038/90216	journal	July 2001
Molecular characterization with carbon nanotube control Golovchenko, Jene A.; Branton, Daniel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7468271 US Patent Document 7,468,271	patent	December 2008
Electrokinetic measurements with submicron particles and pores by the resistive pulse technique DeBlois, Ralph W.; Bean, Charles P.; Wesley, Roy K. A. Journal of Colloid and Interface Science, Vol. 61, Issue 2, p. 323-335 https://doi.org/10.1016/0021-9797(77)90395-2	journal	September 1977
Methods and products for analyzing polymers Chan, Eugene Y. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6355420 US Patent Document 6,355,420	patent	March 2002
Control of solid state dimensional features Golovchenko, Jene A.; Branton, Daniel; Aziz, Michael J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6783643 US Patent Document 6,783,643	patent	August 2004
Detector for nucleic acid typing and methods of using the same Yeh, Homer R.; Wick, Charles H. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6238866 US Patent Document 6,238,866	patent	May 2001
Arbitrary sequence oligonucleotide fingerprinting Beattie, Kenneth L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6156502 US Patent Document 6,156,502	patent	December 2000
Methods and apparatuses for characterization of single polymers Chan, Eugene Y.; Gleich, Lance C.; Wellman, Parris S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020081744 US Patent Application 09/783472; 20020081744	patent-application	June 2002
Characterization of individual polymer molecules based on monomer-interface interactions Denison, Timothy J.; Sauer, Alexis; Golovchenko, Jene A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6362002 US Patent Document 6,362,002	patent	March 2002
Characterization of individual polymer molecules based on monomer-interface interactions Akeson, Mark A.; Branton, Daniel; Church, George M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7189503 US Patent Document 7,189,503	patent	March 2007
Colloquium: Physical approaches to DNA sequencing and detection Zwolak, Michael; Di Ventra, Massimiliano Reviews of Modern Physics, Vol. 80, Issue 1, p. 141-165 https://doi.org/10.1103/RevModPhys.80.141	journal	January 2008
Adapting to nanoscale events Branton, Daniel; Golovchenko, Jene Nature, Vol. 398, Issue 6729 https://doi.org/10.1038/19412	journal	April 1999
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP Ollis, D. L.; Brick, P.; Hamlin, R. Nature, Vol. 313, Issue 6005 https://doi.org/10.1038/313762a0	journal	February 1985
Neural modeling device Ikematsu, Mineo; Sugiyama, Yukihiro; Iseki, Masahiro https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5378342 US Patent Document 5,378,342	patent	January 1995
Methods for solid-phase amplification of DNA template (SPADT) using multiarrays Rovera, Giovanni; Mukhopadhyay, Sunil https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6221635 US Patent Document 6,221,635	patent	April 2001
DNA conformation and base number simultaneously determined in a nanopore Fologea, Daniel; Brandin, Eric; Uplinger, James ELECTROPHORESIS, Vol. 28, Issue 18 https://doi.org/10.1002/elps.200700047	journal	September 2007
DNA measuring method Kambara, Hideki; Okano, Kazunori; Takahashi, Satoshi https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5356776 US Patent Document 5,356,776	patent	October 1994
Miniature support for thin films containing single channels or nanopores and methods for using same Akeson, Mark A.; Deamer, David W.; Branton, Daniel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6267872 US Patent Document 6,267,872	patent	July 2001
Hydrophilic polymer coating of high pH stability for silica surfaces for suppression of electroendomosis and solute adsorption Hjerten, Stellan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5221447 US Patent Document 5,221,447	patent	June 1993
Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores Sukharev, S. I.; Klenchin, V. A.; Serov, S. M. Biophysical Journal, Vol. 63, Issue 5, p. 1320-1327 https://doi.org/10.1016/S0006-3495(92)81709-5	journal	November 1992
Miniature support for thin films containing single channels or nanopores and methods for using the same Akeson, Mark A.; Deamer, David W.; Branton, Daniel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6746594 US Patent Document 6,746,594	patent	June 2004
Methods and apparatus for characterization of single polymers Chan, Eugene Y.; Gleich, Lance C.; Wellman, Parris S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020039737 US Patent Application 09/875779; 20020039737	patent-application	April 2002
Rapid nanopore discrimination between single polynucleotide molecules Meller, A.; Nivon, L.; Brandin, E. Proceedings of the National Academy of Sciences, Vol. 97, Issue 3 https://doi.org/10.1073/pnas.97.3.1079	journal	February 2000
Selective Ion Passage through Functionalized Graphene Nanopores Sint, Kyaw; Wang, Boyang; Král, Petr Journal of the American Chemical Society, Vol. 130, Issue 49, p. 16448-16449 https://doi.org/10.1021/ja804409f	journal	December 2008
Study of polymer molecules and conformations with a nanopore Golovchenko, Jene A.; Li, Jiali; Stein, Derek https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7846738 US Patent Document 7,846,738	patent	December 2010
The potential and challenges of nanopore sequencing Branton, Daniel; Deamer, David W.; Marziali, Andre Nature Biotechnology, Vol. 26, Issue 10 https://doi.org/10.1038/nbt.1495	journal	October 2008
Electrochemical microsensors and method of making such sensors Smith, Rosemary L.; Collins, Scott https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4874499 US Patent Document 4,874,499	patent	October 1989
Control of solid state dimensional features Golovchenko, Jene A.; Branton, Daniel; Stein, Derek https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6464842 US Patent Document 6,464,842	patent	October 2002
Aqueous Stabilization and Self-Assembly of Graphene Sheets into Layered Bio-Nanocomposites using DNA Patil, Avinash J.; Vickery, Jemma L.; Scott, Thomas B. Advanced Materials, Vol. 21, Issue 31, p. 3159-3164 https://doi.org/10.1002/adma.200803633	journal	August 2009
Methods for the detection of nucleic acids Shuber, Anthony P.; Lapidus, Stanley N.; Daley, George Q. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6203993 US Patent Document 6,203,993	patent	March 2001
Sequence-specific detection of individual DNA strands using engineered nanopores Howorka, Stefan; Cheley, Stephen; Bayley, Hagan Nature Biotechnology, Vol. 19, Issue 7 https://doi.org/10.1038/90236	journal	July 2001
Hydrophobic substitution mutations in the S4 sequence alter voltage-dependent gating in shaker K⁺ channels Lopez, George A.; Ian, Yuh Nung; Ian, Lily Yeh Neuron, Vol. 7, Issue 2, p. 327-336 https://doi.org/10.1016/0896-6273(91)90271-Z	journal	August 1991
Multiple-aperture particle counting sizing and deformability-measuring apparatus Fisher, Timothy C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5376878 US Patent Document 5,376,878	patent	December 1994
Methods of analyzing polymers using ordered label strategies Chan, Eugene Y. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6403311 US Patent Document 6,403,311	patent	June 2002
Characterization of individual polymer molecules based on monomer-interface interactions Denison, Timothy J.; Sauer, Alexis; Golovchenko, Jene A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6673615 US Patent Document 6,673,615	patent	January 2004
Open channel noise. III. High-resolution recordings show rapid current fluctuations in gramicidin A and four chemical analogues Sigworth, F. J.; Urry, D. W.; Prasad, K. U. Biophysical Journal, Vol. 52, Issue 6, p. 1055-1064 https://doi.org/10.1016/S0006-3495(87)83299-X	journal	December 1987
Receptor-based sensor Fare, Thomas L.; Ligler, Frances S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5111221 US Patent Document 5,111,221	patent	May 1992
Rolling circle amplification assay for nucleic acid analysis Mahtani, Melanie M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6221603 US Patent Document 6,221,603	patent	April 2001
Material deposition techniques for control of solid state aperture surface properties Branton, Daniel; Gordon, Roy G.; Chen, Peng https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050241933 US Patent Application 11/015349; 20050241933	patent-application	November 2005
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches Hamill, O. P.; Marty, A.; Neher, E. Pflügers Archiv - European Journal of Physiology, Vol. 391, Issue 2 https://doi.org/10.1007/BF00656997	journal	August 1981
Electronic Counting and Sizing of Bacteria Kubitschek, Herbert E. Nature, Vol. 182, Issue 4630 https://doi.org/10.1038/182234a0	journal	July 1958
Characterization of individual polymer molecules based on monomer-interface interactions Baldarelli, Richard; Branton, Daniel; Church, George M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6015714 US Patent Document 6,015,714	patent	January 2000
Methods of determining the presence of double stranded nucleic acids in a sample Deamer, David W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6428959 US Patent Document 6,428,959	patent	August 2002
Channeling phage DNA through membranes: from in vivo to in vitro Letellier, Lucienne; Boulanger, Pascale; de Frutos, Marta Research in Microbiology, Vol. 154, Issue 4, p. 283-287 https://doi.org/10.1016/S0923-2508(03)00072-X	journal	May 2003
Selectivity for maltose and maltodextrins of maltoporin, a pore-forming protein of E. coli outer membrane Dargent, Bénédicte; Rosenbusch, Jürg; Pattus, Franc FEBS Letters, Vol. 220, Issue 1, p. 136-142 https://doi.org/10.1016/0014-5793(87)80891-8	journal	August 1987
Study of polymer molecules and conformations with a nanopore Golovchenko, Jene A.; Li, Jiali; Stein, Derek https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8394640 US Patent Document 8,394,640	patent	March 2013
SpoIIIE strips proteins off the DNA during chromosome translocation Marquis, K. A.; Burton, B. M.; Nollmann, M. Genes & Development, Vol. 22, Issue 13 https://doi.org/10.1101/gad.1684008	journal	July 2008
Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes Simons, Malcolm James https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5612179 US Patent Document 5,612,179	patent	March 1997
Single Ion-Channel Recordings Using Glass Nanopore Membranes White, Ryan J.; Ervin, Eric N.; Yang, Tinglu Journal of the American Chemical Society, Vol. 129, Issue 38 https://doi.org/10.1021/ja073174q	journal	September 2007
Biophysical and molecular mechanisms of Shaker potassium channel inactivation Hoshi, T.; Zagotta, W.; Aldrich, R. Science, Vol. 250, Issue 4980 https://doi.org/10.1126/science.2122519	journal	October 1990
Lithographic fabrication of nanoapertures Fleming, James G. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6503409 US Patent Document 6,503,409	patent	January 2003
Counting polymers moving through a single ion channel Bezrukov, Sergey M.; Vodyanoy, Igor; Parsegian, V. Adrian Nature, Vol. 370, Issue 6487 https://doi.org/10.1038/370279a0	journal	July 1994

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