Yeast cell surface display for lipase whole cell catalyst and its applications
Abstract
The cell surface display technique allows for the expression of target proteins or peptides on the microbial cell surface by fusing an appropriate protein as an anchoring motif. Yeast display systems, such as Pichia pastoris, Yarowia lipolytica and Saccharomyces cerevisiae, are ideal, alternative and extensive display systems with the advantage of simple genetic manipulation and post-translational modification of expressed heterologous proteins. Engineered yeasts show high performance characteristics and variant utilizations. Herein, we comprehensively summarize the variant factors affecting lipase whole cell catalyst activity and display efficiency, including the structure and size of target proteins, screening anchor proteins, type and chain length of linkers, and the appropriate matching rules among the above-mentioned display units. Furthermore, we also address novel approaches to enhance stability and activity of recombinant lipases, such as VHb gene co-expression, multi-enzyme co-display technique, and the micro-environmental interference and self-assembly techniques. Finally, we represent the variety of applications of whole cell surface displayed lipases on yeast cells in non-aqueous phases, including synthesis of esters, PUFA enrichment, resolution of chiral drugs, organic synthesis and biofuels. We demonstrate that the lipase surface display technique is a powerful tool for functionalizing yeasts to serve as whole cell catalysts, and increasing interest ismore »
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1170471
- Report Number(s):
- PNNL-SA-102321
47292; KP1601010
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Journal of Molecular Catalysis B: Enzymatic, 106:17-25
- Additional Journal Information:
- Journal Name: Journal of Molecular Catalysis B: Enzymatic, 106:17-25
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Whole cell catalyst; Lipase; Surface display technique; Yeast cell; Application; Environmental Molecular Sciences Laboratory
Citation Formats
Liu, Yun, Zhang, Rui, Lian, Zhongshuai, Wang, Shihui, and Wright, Aaron T. Yeast cell surface display for lipase whole cell catalyst and its applications. United States: N. p., 2014.
Web. doi:10.1016/j.molcatb.2014.04.011.
Liu, Yun, Zhang, Rui, Lian, Zhongshuai, Wang, Shihui, & Wright, Aaron T. Yeast cell surface display for lipase whole cell catalyst and its applications. United States. https://doi.org/10.1016/j.molcatb.2014.04.011
Liu, Yun, Zhang, Rui, Lian, Zhongshuai, Wang, Shihui, and Wright, Aaron T. 2014.
"Yeast cell surface display for lipase whole cell catalyst and its applications". United States. https://doi.org/10.1016/j.molcatb.2014.04.011.
@article{osti_1170471,
title = {Yeast cell surface display for lipase whole cell catalyst and its applications},
author = {Liu, Yun and Zhang, Rui and Lian, Zhongshuai and Wang, Shihui and Wright, Aaron T.},
abstractNote = {The cell surface display technique allows for the expression of target proteins or peptides on the microbial cell surface by fusing an appropriate protein as an anchoring motif. Yeast display systems, such as Pichia pastoris, Yarowia lipolytica and Saccharomyces cerevisiae, are ideal, alternative and extensive display systems with the advantage of simple genetic manipulation and post-translational modification of expressed heterologous proteins. Engineered yeasts show high performance characteristics and variant utilizations. Herein, we comprehensively summarize the variant factors affecting lipase whole cell catalyst activity and display efficiency, including the structure and size of target proteins, screening anchor proteins, type and chain length of linkers, and the appropriate matching rules among the above-mentioned display units. Furthermore, we also address novel approaches to enhance stability and activity of recombinant lipases, such as VHb gene co-expression, multi-enzyme co-display technique, and the micro-environmental interference and self-assembly techniques. Finally, we represent the variety of applications of whole cell surface displayed lipases on yeast cells in non-aqueous phases, including synthesis of esters, PUFA enrichment, resolution of chiral drugs, organic synthesis and biofuels. We demonstrate that the lipase surface display technique is a powerful tool for functionalizing yeasts to serve as whole cell catalysts, and increasing interest is providing an impetus for broad application of this technique.},
doi = {10.1016/j.molcatb.2014.04.011},
url = {https://www.osti.gov/biblio/1170471},
journal = {Journal of Molecular Catalysis B: Enzymatic, 106:17-25},
number = ,
volume = ,
place = {United States},
year = {Fri Aug 01 00:00:00 EDT 2014},
month = {Fri Aug 01 00:00:00 EDT 2014}
}