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Title: Expanding the eukaryotic genetic code

Patent ·
OSTI ID:1169093
; ; ;

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

Research Organization:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG0300ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,945,926
Application Number:
13/329,202
OSTI ID:
1169093
Country of Publication:
United States
Language:
English

References (90)

Glycoprotein synthesis patent August 2005
In vivo incorporation of unnatural amino acids patent May 2006
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs patent August 2006
Glycoprotein synthesis patent October 2006
Glycoprotein synthesis patent February 2007
Glycoprotein synthesis patent April 2007
Glycoprotein synthesis patent May 2007
Glycoprotein synthesis patent July 2007
Glycoprotein synthesis patent August 2007
Expanding the eukaryotic genetic code patent November 2009
Glycoprotein synthesis patent November 2010
patent-application October 2004
Unnatural reactive amino acid genetic code additions patent-application December 2004
Expanding the eukaryotic genetic code patent-application January 2005
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application June 2005
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application September 2005
Site-specific incorporation of redox active amino acids into proteins patent-application October 2005
Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination patent-application December 2005
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application March 2006
Site specific incorporation of keto amino acids into proteins patent-application March 2006
In vivo incorporation of alkynyl amino acids into proteins in eubacteria patent-application April 2006
Site-specific labeling of proteins for NMR studies patent-application May 2006
In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria patent-application May 2006
Adding photoregulated amino acids to the genetic code patent-application June 2006
Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application July 2006
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof patent-application August 2006
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application October 2006
Unnatural reactive amino acid genetic code additions patent-application November 2006
Site-specific incorporation of redox active amino acids into proteins patent-application January 2007
Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application January 2007
Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application February 2007
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application May 2007
Expanding the eukaryotic genetic code patent-application July 2007
Expanding the eukaryotic genetic code patent-application July 2007
Glycoprotein synthesis patent-application July 2007
Selective posttranslational modification of phage-displayed polypeptides patent-application August 2007
Glycoprotein synthesis patent-application August 2007
Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine patent-application October 2007
Site specific incorporation of keto amino acids into proteins patent-application September 2008
In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris patent-application August 2009
Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs journal September 1990
Expanding tRNA recognition of a tRNA synthetase by a single amino acid change journal May 2003
Programming peptidomimetic syntheses by translating genetic codes designed de novo journal May 2003
Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation journal November 2002
Misacylation of Yeast Amber Suppressor tRNATyr by E. coli Lysyl-tRNA Synthetase and Its Effective Repression by Genetic Engineering of the tRNA Sequence journal April 2006
Site-specific incorporation of biotinylated amino acids to identify surface-exposed residues in integral membrane proteins journal October 1997
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells journal July 1998
A Mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine journal September 2000
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system journal July 2002
Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation journal December 2001
Click Chemistry: Diverse Chemical Function from a Few Good Reactions journal June 2001
Click Chemistry In Situ: Acetylcholinesterase as a Reaction Vessel for the Selective Assembly of a Femtomolar Inhibitor from an Array of Building Blocks journal March 2002
Progress toward the evolution of an organism with an expanded genetic code journal April 1999
A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code journal September 2000
Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis journal May 1997
Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator. journal August 1989
Decomposition of Protein Tryptophan Fluorescence Spectra into Log-Normal Components. II. The Statistical Proof of Discreteness of Tryptophan Classes in Proteins journal September 2001
A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective “Ligation” of Azides and Terminal Alkynes journal July 2002
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells journal November 2002
Activity-Based Protein Profiling in Vivo Using a Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
  • Speers, Anna E.; Adam, Gregory C.; Cravatt, Benjamin F.
  • Journal of the American Chemical Society, Vol. 125, Issue 16, p. 4686-4687 https://doi.org/10.1021/ja034490h
journal April 2003
Peptidotriazoles on Solid Phase  [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides journal May 2002
Protein Tyrosine Kinase Csk-Catalyzed Phosphorylation of Src Containing Unnatural Tyrosine Analogues journal September 2001
Expanding the Genetic Code of Escherichia coli journal April 2001
Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli journal March 2002
Addition of the keto functional group to the genetic code of Escherichia coli journal December 2002
Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition journal March 2003
Imaging biochemistry inside cells journal May 2001
Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins journal November 2001
Exploring the Limits of Codon and Anticodon Size journal February 2002
Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286
  • Arslan, Tuncer; Mamaev, Sergey V.; Mamaeva, Natalia V.
  • Journal of the American Chemical Society, Vol. 119, Issue 45, p. 10877-10887 https://doi.org/10.1021/ja971927a
journal November 1997
Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury journal January 1996
Aliphatic amino azides as key building blocks for efficient polyamine syntheses journal July 1993
Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
  • Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
  • Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027 https://doi.org/10.1021/ja027007w
journal July 2002
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal August 2002
An Expanded Eukaryotic Genetic Code journal August 2003
Progress Toward an Expanded Eukaryotic Genetic Code journal June 2003
Site-Specific Protein Modification Using a Ketone Handle
  • Cornish, Virginia W.; Hahn, Klaus M.; Schultz, Peter G.
  • Journal of the American Chemical Society, Vol. 118, Issue 34, p. 8150-8151 https://doi.org/10.1021/ja961216x
journal January 1996
Preparation of biological labels with acetylenic linker arms journal January 1997
Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
  • Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
  • Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783 https://doi.org/10.1021/ja0370037
journal October 2003
A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase. journal April 1990
Recent advances in technology for measuring and manipulating cell signals journal June 2000
The Selective Incorporation of Alkenes into Proteins in Escherichia coli journal August 2002
A New Strategy for the Site-Specific Modification of Proteins in Vivo journal June 2003
Chemoselective ligation reactions with proteins, oligosaccharides and cells journal December 1998
Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli journal March 2001
Expanding the Genetic code journal June 2006
Cellular screening assays using fluorescence microscopy journal October 2001
In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis journal November 2002
An expanding genetic code journal December 2004
Unnatural amino acids as probes of protein structure and function journal December 2000

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