Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study

Sampath, Sujatha; Yarger, Jeffery L.

doi:10.1039/C4RA13936D

Title: Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study

Journal Article · Thu Nov 27 00:00:00 EST 2014 · RSC Adv.

DOI:https://doi.org/10.1039/C4RA13936D· OSTI ID:1168489

Sampath, Sujatha ^[1]; Yarger, Jeffery L. ^[2]

Univ. of Wisconsin-Milwaukee, WI (United States). Dept. of Physics; Arizona State Univ., Tempe, AZ (United States). Dept. of Chemistry and Biochemistry
Arizona State Univ., Tempe, AZ (United States). Dept. of Chemistry and Biochemistry

Interaction with water causes shrinkage and significant changes in the structure of spider dragline silks, which has been referred to as supercontraction in the literature. Preferred orientation or alignment of protein chains with respect to the fiber axis is extensively changed during this supercontraction process. Synchrotron X-ray micro-fiber diffraction experiments have been performed on Nephila clavipes and Argiope aurantia major and minor ampullate dragline spider fibers in the native dry, contracted (by immersion in water) and restretched (from contracted) states. Changes in the orientation of β-sheet nanocrystallites and the oriented component of the amorphous network have been determined from wide-angle X-ray diffraction patterns. While both the crystalline and amorphous components lose preferred orientation on wetting with water, the nano-crystallites regain their orientation on wet-restretching, whereas the oriented amorphous components only partially regain their orientation. Dragline major ampullate silks in both the species contract more than their minor ampullate silks.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOD; National Science Foundation (NSF)

Grant/Contract Number:: AC02-06CH11357; SC0004791

OSTI ID:: 1168489

Journal Information:: RSC Adv., Vol. 5, Issue (2) ; 2015

Country of Publication:: United States

Language:: ENGLISH

Citation Metrics:

Cited by: 16 works

Citation information provided by
Web of Science

References (55)

Dimensions, Birefringences, and Force-Elongation Behavior of Major and Minor Ampullate Silk Fibers from Orb-Web-Spinning Spiders—The Effects of Wetting on these Properties Work, Robert W. Textile Research Journal, Vol. 47, Issue 10 https://doi.org/10.1177/004051757704701003	journal	October 1977
Relationships between supercontraction and mechanical properties of spider silk Liu, Yi; Shao, Zhengzhong; Vollrath, Fritz Nature Materials, Vol. 4, Issue 12 https://doi.org/10.1038/nmat1534	journal	November 2005
Self-tightening of spider silk fibers induced by moisture Guinea, G. V.; Elices, M.; Pérez-Rigueiro, J. Polymer, Vol. 44, Issue 19 https://doi.org/10.1016/S0032-3861(03)00625-6	journal	September 2003
How super is supercontraction? Persistent versus cyclic responses to humidity in spider dragline silk Blackledge, T. A.; Boutry, C.; Wong, S. -C. Journal of Experimental Biology, Vol. 212, Issue 13 https://doi.org/10.1242/jeb.028944	journal	June 2009
Recovery in spider silk fibers: Recovery in Spider Silk Fibers Elices, M.; Pérez-Rigueiro, J.; Plaza, G. Journal of Applied Polymer Science, Vol. 92, Issue 6 https://doi.org/10.1002/app.20383	journal	March 2004
The hidden link between supercontraction and mechanical behavior of spider silks Elices, Manuel; Plaza, Gustavo R.; Pérez-Rigueiro, José Journal of the Mechanical Behavior of Biomedical Materials, Vol. 4, Issue 5 https://doi.org/10.1016/j.jmbbm.2010.09.008	journal	July 2011
Evolution of supercontraction in spider silk: structure-function relationship from tarantulas to orb-weavers Boutry, C.; Blackledge, T. A. Journal of Experimental Biology, Vol. 213, Issue 20 https://doi.org/10.1242/jeb.046110	journal	October 2010
Spider Silk: Ancient Ideas for New Biomaterials Lewis, Randolph V. Chemical Reviews, Vol. 106, Issue 9 https://doi.org/10.1021/cr010194g	journal	September 2006
Liquid crystalline spinning of spider silk Vollrath, Fritz; Knight, David P. Nature, Vol. 410, Issue 6828 https://doi.org/10.1038/35069000	journal	March 2001
Hierarchical structures made of proteins. The complex architecture of spider webs and their constituent silk proteins Heim, Markus; Römer, Lin; Scheibel, Thomas Chem. Soc. Rev., Vol. 39, Issue 1 https://doi.org/10.1039/B813273A	journal	January 2010
Decoding the secrets of spider silk Eisoldt, Lukas; Smith, Andrew; Scheibel, Thomas Materials Today, Vol. 14, Issue 3 https://doi.org/10.1016/S1369-7021(11)70057-8	journal	March 2011
Tu(r)ning weakness to strength Buehler, Markus J. Nano Today, Vol. 5, Issue 5 https://doi.org/10.1016/j.nantod.2010.08.001	journal	January 2010
Supercontraction and Backbone Dynamics in Spider Silk: ¹³ C and ² H NMR Studies Yang, Zhitong; Liivak, Oskar; Seidel, Andreas Journal of the American Chemical Society, Vol. 122, Issue 37 https://doi.org/10.1021/ja0017099	journal	September 2000
Spider silk as rubber Gosline, John M.; Denny, Mark W.; DeMont, M. Edwin Nature, Vol. 309, Issue 5968 https://doi.org/10.1038/309551a0	journal	June 1984
Molecular Orientation and Two-Component Nature of the Crystalline Fraction of Spider Dragline Silk Simmons, A. H.; Michal, C. A.; Jelinski, L. W. Science, Vol. 271, Issue 5245 https://doi.org/10.1126/science.271.5245.84	journal	January 1996
Molecular Modeling of Spider Silk Elasticity Termonia, Yves Macromolecules, Vol. 27, Issue 25 https://doi.org/10.1021/ma00103a018	journal	December 1994
Molecular chain orientation in supercontracted and re-extended spider silk Grubb, D. T.; Ji, Gending International Journal of Biological Macromolecules, Vol. 24, Issue 2-3 https://doi.org/10.1016/S0141-8130(98)00086-5	journal	March 1999
Combined structural model of spider dragline silk Ene, Roxana; Papadopoulos, Periklis; Kremer, Friedrich Soft Matter, Vol. 5, Issue 22 https://doi.org/10.1039/b911159j	journal	January 2009
Spider silk: the unraveling of a mystery Lewis, Randolph V. Accounts of Chemical Research, Vol. 25, Issue 9 https://doi.org/10.1021/ar00021a002	journal	September 1992
Stretching of supercontracted fibers: a link between spinning and the variability of spider silk Guinea, G. V. Journal of Experimental Biology, Vol. 208, Issue 1 https://doi.org/10.1242/jeb.01344	journal	January 2005
Spidroins from the Brazilian spider Nephilengys cruentata (Araneae: Nephilidae) Bittencourt, D.; Souto, B. M.; Verza, N. C. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Vol. 147, Issue 4 https://doi.org/10.1016/j.cbpb.2007.03.013	journal	August 2007
Mechanical Behavior of Silk During the Evolution of Orb-Web Spinning Spiders Elices, Manuel; Plaza, Gustavo R.; Arnedo, Miquel A. Biomacromolecules, Vol. 10, Issue 7 https://doi.org/10.1021/bm900312c	journal	July 2009
Creep and low Strength of Spider Dragline Subjected to Constant Loads Smith, Christopher; Ritchie, Joanne; Bell, Fraser I. Journal of Arachnology, Vol. 31, Issue 3 https://doi.org/10.1636/H02-46	journal	December 2003
Supercontraction Stress in Spider Webs Savage, Ken N.; Guerette, Paul A.; Gosline, John M. Biomacromolecules, Vol. 5, Issue 3 https://doi.org/10.1021/bm034270w	journal	May 2004
Minor Ampullate Silks from Nephila and Argiope Spiders: Tensile Properties and Microstructural Characterization Guinea, G. V.; Elices, M.; Plaza, G. R. Biomacromolecules, Vol. 13, Issue 7 https://doi.org/10.1021/bm3004644	journal	June 2012
Structural studies of spider silk proteins in the fiber Parkhe, Ajay D.; Seeley, Stacy K.; Gardner, Kenneth Journal of Molecular Recognition, Vol. 10, Issue 1 https://doi.org/10.1002/(SICI)1099-1352(199701/02)10:1<1::AID-JMR338>3.0.CO;2-7	journal	January 1997
Solid-State NMR Investigation of Major and Minor Ampullate Spider Silk in the Native and Hydrated States Holland, Gregory P.; Jenkins, Janelle E.; Creager, Melinda S. Biomacromolecules, Vol. 9, Issue 2 https://doi.org/10.1021/bm700950u	journal	February 2008
Fiber Morphology of Spider Silk: The Effects of Tensile Deformation Grubb, David T.; Jelinski, Lynn W. Macromolecules, Vol. 30, Issue 10 https://doi.org/10.1021/ma961293c	journal	May 1997
Aspects of X-ray diffraction on single spider fibers Riekel, C.; Bränden, C.; Craig, C. International Journal of Biological Macromolecules, Vol. 24, Issue 2-3 https://doi.org/10.1016/S0141-8130(98)00084-1	journal	March 1999
X-ray diffraction from single fibres of spider silk Bram, A.; Brändén, C. I.; Craig, C. Journal of Applied Crystallography, Vol. 30, Issue 3 https://doi.org/10.1107/S0021889896012344	journal	June 1997
In Situ X-ray Diffraction during Forced Silking of Spider Silk Riekel, Christian; Müller, Martin; Vollrath, Fritz Macromolecules, Vol. 32, Issue 13 https://doi.org/10.1021/ma990067a	journal	June 1999
X-ray diffraction study of nanocrystalline and amorphous structure within major and minor ampullate dragline spider silks Sampath, Sujatha; Isdebski, Thomas; Jenkins, Janelle E. Soft Matter, Vol. 8, Issue 25 https://doi.org/10.1039/c2sm25373a	journal	January 2012
Supercontraction stress in wet spider dragline Bell, Fraser I.; McEwen, Iain J.; Viney, Christopher Nature, Vol. 416, Issue 6876 https://doi.org/10.1038/416037a	journal	March 2002
Variability in the mechanical properties of spider silks on three levels: interspecific, intraspecific and intraindividual Madsen, Bo; Shao, Zheng Zhong; Vollrath, Fritz International Journal of Biological Macromolecules, Vol. 24, Issue 2-3 https://doi.org/10.1016/S0141-8130(98)00094-4	journal	March 1999
The effect of spinning conditions on the mechanics of a spider's dragline silk Vollrath, Fritz; Madsen, Bo; Shao, Zhengzhong Proceedings of the Royal Society of London. Series B: Biological Sciences, Vol. 268, Issue 1483 https://doi.org/10.1098/rspb.2001.1590	journal	November 2001
Accurate New Method for Molecular Orientation Quantification Using Polarized Raman Spectroscopy Richard-Lacroix, Marie; Pellerin, Christian Macromolecules, Vol. 46, Issue 14 https://doi.org/10.1021/ma400955u	journal	June 2013
High Mobility Field Effect Transistors Based on Macroscopically Oriented Regioregular Copolymers Tseng, Hsin-Rong; Ying, Lei; Hsu, Ben B. Y. Nano Letters, Vol. 12, Issue 12 https://doi.org/10.1021/nl303612z	journal	February 2012
Molecular origins of anisotropy in the thermal conductivity of deformed polymer melts: stress versus orientation contributions Schieber, Jay D.; Venerus, David C.; Gupta, Sahil Soft Matter, Vol. 8, Issue 47 https://doi.org/10.1039/c2sm26788h	journal	January 2012
Shrinkage and chain folding in drawn poly(ethylene terephthalate) fibres Wilson, M. P. W. Polymer, Vol. 15, Issue 5 https://doi.org/10.1016/0032-3861(74)90124-4	journal	May 1974
Oriented noncrystalline structure in PET fibers prepared with threadline modification process Wu, Gang; Jiang, Jiao-Dong; Tucker, Paul A. Journal of Polymer Science Part B: Polymer Physics, Vol. 34, Issue 12 https://doi.org/10.1002/(SICI)1099-0488(19960915)34:12<2035::AID-POLB9>3.0.CO;2-Y	journal	September 1996
Structure of heat-treated Nylon 6 and 6.6 fibers. I. The shrinkage mechanism Simal, Abigail Lisb�o; Martin, Adriana Regina Journal of Applied Polymer Science, Vol. 68, Issue 3 https://doi.org/10.1002/(SICI)1097-4628(19980418)68:3<441::AID-APP11>3.0.CO;2-Y	journal	April 1998
The effect of solvents on the contraction and mechanical properties of spider silk Shao, Zhengzhong; Vollrath, Fritz Polymer, Vol. 40, Issue 7 https://doi.org/10.1016/S0032-3861(98)00266-3	journal	March 1999
Structure of a protein superfiber: spider dragline silk. Xu, M.; Lewis, R. V. Proceedings of the National Academy of Sciences, Vol. 87, Issue 18 https://doi.org/10.1073/pnas.87.18.7120	journal	September 1990
Hypotheses that correlate the sequence, structure, and mechanical properties of spider silk proteins Hayashi, Cheryl Y.; Shipley, Nichola H.; Lewis, Randolph V. International Journal of Biological Macromolecules, Vol. 24, Issue 2-3 https://doi.org/10.1016/S0141-8130(98)00089-0	journal	March 1999
Quantitative Correlation between the Protein Primary Sequences and Secondary Structures in Spider Dragline Silks Jenkins, Janelle E.; Creager, Melinda S.; Lewis, Randolph V. Biomacromolecules, Vol. 11, Issue 1 https://doi.org/10.1021/bm9010672	journal	January 2010
2H–13C HETCOR MAS NMR for indirect detection of 2H quadrupole patterns and spin–lattice relaxation rates Shi, Xiangyan; Yarger, Jeffery L.; Holland, Gregory P. Journal of Magnetic Resonance, Vol. 226 https://doi.org/10.1016/j.jmr.2012.10.013	journal	January 2013
Silken toolkits: biomechanics of silk fibers spun by the orb web spider Argiope argentata (Fabricius 1775) Blackledge, T. A. Journal of Experimental Biology, Vol. 209, Issue 13 https://doi.org/10.1242/jeb.02275	journal	July 2006
An Investigation of the Divergence of Major Ampullate Silk Fibers from Nephila clavipes and Argiope aurantia Brooks, Amanda E.; Steinkraus, Holly B.; Nelson, Shane R. Biomacromolecules, Vol. 6, Issue 6 https://doi.org/10.1021/bm050421e	journal	November 2005
Proline and Processing of Spider Silks Liu, Yi; Sponner, Alexander; Porter, David Biomacromolecules, Vol. 9, Issue 1 https://doi.org/10.1021/bm700877g	journal	January 2008
Elucidating proline dynamics in spider dragline silk fibre using ² H– ¹³ C HETCOR MAS NMR Shi, Xiangyan; Yarger, Jeffery L.; Holland, Gregory P. Chem. Commun., Vol. 50, Issue 37 https://doi.org/10.1039/C4CC00971A	journal	January 2014
The effect of proline on the network structure of major ampullate silks as inferred from their mechanical and optical properties Savage, K. N.; Gosline, J. M. Journal of Experimental Biology, Vol. 211, Issue 12 https://doi.org/10.1242/jeb.014217	journal	June 2008
Two Mechanisms for Supercontraction in Nephila Spider Dragline Silk Guan, Juan; Vollrath, Fritz; Porter, David Biomacromolecules, Vol. 12, Issue 11 https://doi.org/10.1021/bm201032v	journal	November 2011
Protein Composition Correlates with the Mechanical Properties of Spider (Argiope trifasciata) Dragline Silk Marhabaie, Mohammad; Leeper, Thomas C.; Blackledge, Todd A. Biomacromolecules, Vol. 15, Issue 1 https://doi.org/10.1021/bm401110b	journal	December 2013
Supercontraction of dragline silk spun by lynx spiders (Oxyopidae) Pérez-Rigueiro, J.; Plaza, G. R.; Torres, F. G. International Journal of Biological Macromolecules, Vol. 46, Issue 5 https://doi.org/10.1016/j.ijbiomac.2010.03.013	journal	June 2010
Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR and X-ray Diffraction Jenkins, Janelle E.; Sampath, Sujatha; Butler, Emily Biomacromolecules, Vol. 14, Issue 10 https://doi.org/10.1021/bm400791u	journal	September 2013

Cited By (5)

Experimental Methods for Characterizing the Secondary Structure and Thermal Properties of Silk Proteins McGill, Meghan; Holland, Gregory P.; Kaplan, David L. Macromolecular Rapid Communications, Vol. 40, Issue 1 https://doi.org/10.1002/marc.201800390	journal	August 2018
Phase transitions as intermediate steps in the formation of molecularly engineered protein fibers Mohammadi, Pezhman; Aranko, A. Sesilja; Lemetti, Laura Communications Biology, Vol. 1, Issue 1 https://doi.org/10.1038/s42003-018-0090-y	journal	July 2018
The transcriptome of Darwin’s bark spider silk glands predicts proteins contributing to dragline silk toughness Garb, Jessica E.; Haney, Robert A.; Schwager, Evelyn E. Communications Biology, Vol. 2, Issue 1 https://doi.org/10.1038/s42003-019-0496-1	journal	July 2019
Effect of stress on the molecular structure and mechanical properties of supercontracted spider dragline silks Dong, Qinglin; Fang, Guangqiang; Huang, Yufang Journal of Materials Chemistry B, Vol. 8, Issue 1 https://doi.org/10.1039/c9tb02032b	journal	January 2020
Using hydrodynamic focusing to predictably alter the diameter of synthetic silk fibers Hoffmann, Bradley; Gruat-Henry, Catherine; Mulinti, Pranothi PLOS ONE, Vol. 13, Issue 4 https://doi.org/10.1371/journal.pone.0195522	journal	April 2018

Similar Records

X-ray diffraction study of nanocrystalline and amorphous structure within major and minor ampullate dragline spider silks

Journal Article · Wed Jul 25 00:00:00 EDT 2012 · Soft Matter · OSTI ID:1168489

Sampath, Sujatha; Isdebski, Thomas; Jenkins, Janelle E; +4 more

Reproducing Natural Spider Silks' Copolymer Behavior in Synthetic Silk Mimics

Journal Article · Tue Oct 30 00:00:00 EDT 2012 · Biomacromolecules · OSTI ID:1168489

An, Bo; Jenkins, Janelle E; Sampath, Sujatha; +4 more

Crystal Structure of the Nephila clavipes Major Ampullate Spidroin 1A N-terminal Domain Reveals Plasticity at the Dimer Interface

Journal Article · Thu Jul 21 00:00:00 EDT 2016 · Journal of Biological Chemistry · OSTI ID:1168489

Atkison, James H.; Parnham, Stuart; Marcotte, Jr., William R.; +1 more

Related Subjects

36 MATERIALS SCIENCE

Title: Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study

Citation Formats

References (55)

Cited By (5)

Similar Records

Related Subjects