Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby

Tsien, Roger Y; Wang, Lei

Title: Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby

Patent · Tue Jan 13 00:00:00 EST 2015

OSTI ID:1167214

Tsien, Roger Y; Wang, Lei

Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.

View Patent

Cite

Export

Save

Research Organization:: Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG03-01ER63276

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 8,932,859

Application Number:: 12/124,888

OSTI ID:: 1167214

Resource Relation:: Patent File Date: 2008 May 21

Country of Publication:: United States

Language:: English

References (28)

Modified green fluorescent proteins Tsien, Roger Y.; Heim, Roger https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5625048 US Patent Document 5,625,048	patent	April 1997
Directed evolution of protein in mammalian cells Peelle, Beau https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6723537 US Patent Document 6,723,537	patent	April 2004
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Gossen, M.; Bujard, H. Proceedings of the National Academy of Sciences, Vol. 89, Issue 12 https://doi.org/10.1073/pnas.89.12.5547	journal	June 1992
CD40 Stimulation of Human Peripheral B Lymphocytes: Distinct Response from Naive and Memory Cells Fecteau, Jessie F.; Néron, Sonia The Journal of Immunology, Vol. 171, Issue 9 https://doi.org/10.4049/jimmunol.171.9.4621	journal	October 2003
Hypermutation targets a green fluorescent protein-encoding transgene in the presence of immunoglobulin enhancers Bachl, Jürgen; Olsson, Carina European Journal of Immunology, Vol. 29, Issue 4, p. 1383-1389 https://doi.org/10.1002/(SICI)1521-4141(199904)29:04<1383::AID-IMMU1383>3.0.CO;2-X	journal	April 1999
Increased Transcription Levels Induce Higher Mutation Rates in a Hypermutating Cell Line Bachl, Jürgen; Carlson, Chris; Gray-Schopfer, Vanessa The Journal of Immunology, Vol. 166, Issue 8 https://doi.org/10.4049/jimmunol.166.8.5051	journal	April 2001
A monomeric red fluorescent protein Campbell, R. E.; Tour, O.; Palmer, A. E. Proceedings of the National Academy of Sciences, Vol. 99, Issue 12, p. 7877-7882 https://doi.org/10.1073/pnas.082243699	journal	June 2002
Somatic Hypermutation in the Heavy Chain Locus Correlates with Transcription Fukita, Yosho; Jacobs, Heinz; Rajewsky, Klaus Immunity, Vol. 9, Issue 1, p. 105-114 https://doi.org/10.1016/S1074-7613(00)80592-0	journal	July 1998
The roots of antibody diversity Gearhart, Patricia J. Nature, Vol. 419, Issue 6902 https://doi.org/10.1038/419029a	journal	September 2002
Death by Deamination: A Novel Host Restriction System for HIV-1 Goff, Stephen P. Cell, Vol. 114, Issue 3, p. 281-283 https://doi.org/10.1016/S0092-8674(03)00602-0	journal	August 2003
RNA Editing Enzyme APOBEC1 and Some of Its Homologs Can Act as DNA Mutators Harris, Reuben S.; Petersen-Mahrt, Svend K.; Neuberger, Michael S. Molecular Cell, Vol. 10, Issue 5, p. 1247-1253 https://doi.org/10.1016/S1097-2765(02)00742-6	journal	November 2002
The generation of antibody diversity through somatic hypermutation and class switch recombination Li, Z. Genes & Development, Vol. 18, Issue 1 https://doi.org/10.1101/gad.1161904	journal	January 2004
Fluorescent proteins from nonbioluminescent Anthozoa species Matz, Mikhail V.; Fradkov, Arkady F.; Labas, Yulii A. Nature Biotechnology, Vol. 17, Issue 10 https://doi.org/10.1038/13657	journal	October 1999
Somatic hypermutation of the AID transgene in B and non-B cells Martin, A.; Scharff, M. D. Proceedings of the National Academy of Sciences, Vol. 99, Issue 19, p. 12304-12308 https://doi.org/10.1073/pnas.192442899	journal	August 2002
Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas Martin, Alberto; Bardwell, Philip D.; Woo, Caroline J. Nature, Vol. 415, Issue 6873 https://doi.org/10.1038/nature714	journal	January 2002
Crystal Structure of the Aequorea victoria Green Fluorescent Protein Orm , M.; Cubitt, A. B.; Kallio, K. Science, Vol. 273, Issue 5280 https://doi.org/10.1126/science.273.5280.1392	journal	September 1996
Hypermutation of multiple proto-oncogenes in B-cell diffuse large-cell lymphomas Pasqualucci, Laura; Neumeister, Peter; Goossens, Tina Nature, Vol. 412, Issue 6844 https://doi.org/10.1038/35085588	journal	July 2001
AID mutates E. coli suggesting a DNA deamination mechanism for antibody diversification Petersen-Mahrt, Svend K.; Harris, Reuben S.; Neuberger, Michael S. Nature, Vol. 418, Issue 6893 https://doi.org/10.1038/nature00862	journal	July 2002
Evolutionary and somatic selection of the antibody repertoire in the mouse Rajewsky, K.; Forster, I.; Cumano, A. Science, Vol. 238, Issue 4830 https://doi.org/10.1126/science.3317826	journal	November 1987
TdT-Accessible Breaks Are Scattered over the Immunoglobulin V Domain in a Constitutively Hypermutating B Cell Line Sale, Julian E.; Neuberger, Michael S. Immunity, Vol. 9, Issue 6, p. 859-869 https://doi.org/10.1016/S1074-7613(00)80651-2	journal	December 1998
Ablation of XRCC2/3 transforms immunoglobulin V gene conversion into somatic hypermutation Sale, Julian E.; Calandrini, Daniella M.; Takata, Minoru Nature, Vol. 412, Issue 6850 https://doi.org/10.1038/35091100	journal	August 2001
GFP-like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity Shagin, Dmitry A.; Barsova, Ekaterina V.; Yanushevich, Yurii G. Molecular Biology and Evolution, Vol. 21, Issue 5 https://doi.org/10.1093/molbev/msh079	journal	May 2004
The TATA binding protein, c-Myc and survivin genes are not somatically hypermutated, while Ig and BCL6 genes are hypermutated in human memory B cells Shen, Hong Ming; Michael, Nancy; Kim, Nayun International Immunology, Vol. 12, Issue 7 https://doi.org/10.1093/intimm/12.7.1085	journal	July 2000
Somatic hypermutation of immunoglobulin and non–immunoglobulin genes Storb, Ursula; Ming Shen, Hong; Michael, Nancy Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 356, Issue 1405 https://doi.org/10.1098/rstb.2000.0743	journal	January 2001
Genome-wide somatic hypermutation Wang, C. L.; Harper, R. A.; Wabl, M. Proceedings of the National Academy of Sciences, Vol. 101, Issue 19, p. 7352-7356 https://doi.org/10.1073/pnas.0402009101	journal	April 2004
Evolution of new nonantibody proteins via iterative somatic hypermutation Wang, L.; Jackson, W. C.; Steinbach, P. A. Proceedings of the National Academy of Sciences, Vol. 101, Issue 48, p. 16745-16749 https://doi.org/10.1073/pnas.0407752101	journal	November 2004
A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins Yanushevich, Yurii G.; Staroverov, Dmitry B.; Savitsky, Alexander P. FEBS Letters, Vol. 511, Issue 1-3, p. 11-14 https://doi.org/10.1016/S0014-5793(01)03263-X	journal	December 2001
Human Activation-Induced Cytidine Deaminase Is Induced by IL-4 and Negatively Regulated by CD45: Implication of CD45 as a Janus Kinase Phosphatase in Antibody Diversification Zhou, Cheng; Saxon, Andrew; Zhang, Ke The Journal of Immunology, Vol. 170, Issue 4 https://doi.org/10.4049/jimmunol.170.4.1887	journal	February 2003

Similar Records

Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same

Patent · Tue Jul 01 00:00:00 EDT 2008 · OSTI ID:1167214

Tsien, Roger Y; Wang, Lei

Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

Patent · Tue Apr 02 00:00:00 EDT 2019 · OSTI ID:1167214

Sweeney, Matthew; Wogulis, Mark

Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

Patent · Tue Nov 14 00:00:00 EST 2017 · OSTI ID:1167214

Sweeney, Matt; Wogulis, Mark

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby

Citation Formats

References (28)

Similar Records

Related Subjects