Detecting and Removing Data Artifacts in Hadamard Transform Ion Mobility-Mass Spectrometry Measurements
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
- Washington State Univ., Pullman, WA (United States). Dept. of Chemistry
Applying Hadamard transform multiplexing to ion mobility separations (IMS) can significantly improve the signal-to-noise ratio and throughput for IMS coupled mass spectrometry (MS) measurements by increasing the ion utilization efficiency. However, it has been determined that both fluctuations in ion intensity as well as spatial shifts in the multiplexed data lower the signal-to-noise ratios and appear as noise in downstream processing of the data. To address this problem, we have developed a novel algorithm that discovers and eliminates data artifacts. The algorithm uses knowledge of the true signal peaks derived from the encoded data and allows for both artifacts and noise to be removed with high confidence, decreasing the likelihood of false identifications in subsequent data processing. The result is that IMS-MS can be applied to increase measurement sensitivity while avoiding artifacts that have previously limited its utility.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Grant/Contract Number:
- AC05-76RLO01830; R01ES022190; R21-CA12619-01; U24-CA-160019-01; 8 P41 GM103493-10; R21 GM103497
- OSTI ID:
- 1166861
- Report Number(s):
- PNNL-SA-100086; 32707; 48135; 400412000
- Journal Information:
- Journal of the American Society for Mass Spectrometry, Vol. 25, Issue 12; ISSN 1044-0305
- Publisher:
- American Society for Mass SpectrometryCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Lipid and Glycolipid Isomer Analyses Using Ultra-High Resolution Ion Mobility Spectrometry Separations
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journal | January 2017 |
Ion Mobility-Mass Spectrometry: Time-Dispersive Instrumentation
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journal | January 2015 |
Influence of multiplexing conditions on artefact signal and the signal-to-noise ratio in the decoded data in Hadamard transform ion mobility spectrometry
|
journal | January 2020 |
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