Improving the Sensitivity of Mass Spectrometry by Using a New Sheath Flow Electrospray Emitter Array at Subambient Pressures

Cox, Jonathan T.; Marginean, Ioan; Kelly, Ryan T.; Smith, Richard D.; Tang, Keqi

doi:10.1007/s13361-014-0856-5

Title: Improving the Sensitivity of Mass Spectrometry by Using a New Sheath Flow Electrospray Emitter Array at Subambient Pressures

Journal Article · Fri Mar 28 00:00:00 EDT 2014 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/s13361-014-0856-5· OSTI ID:1166826

Cox, Jonathan T. ^[1]; Marginean, Ioan ^[1]; Kelly, Ryan T. ^[1]; Smith, Richard D. ^[1]; Tang, Keqi ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division

Arrays of chemically etched emitters with individualized sheath gas capillaries have been developed to enhance electrospray ionization (ESI) at subambient pressures. By including an emitter array in a subambient pressure ionization with nanoelectrospray (SPIN) source, ionization and transmission efficiency can be maximized allowing for increased sensitivity in mass spectrometric analyses. The SPIN source eliminates the major ion losses at conventional ESI-mass spectrometry (MS) interface by placing the emitter in the first vacuum region of the instrument. To facilitate stable electrospray currents in such conditions we have developed an improved emitter array with individualized sheath gas around each emitter. The utility of the new emitter arrays for generating stable multi-electrosprays at subambient pressures was probed by coupling the emitter array/SPIN source with a time of flight (TOF) mass spectrometer. The instrument sensitivity was compared between single emitter/SPIN-MS and multi-emitter/SPIN-MS configurations using an equimolar solution of 9 peptides. An increase in sensitivity correlative to the number of emitters in the array was observed.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; 1R33CA155252; RR18522; 8 P41 GM103493-10; AC05-76RLO01830

OSTI ID:: 1166826

Report Number(s):: PNNL-SA-96045; 48135; 47301; 400412000

Journal Information:: Journal of the American Society for Mass Spectrometry, Vol. 25, Issue 12; ISSN 1044-0305

Publisher:: American Society for Mass SpectrometryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 15 works

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References (33)

Biases in ion transmission through an electrospray ionization-mass spectrometry capillary inlet Page, Jason S.; Marginean, Ioan; Baker, Erin S. Journal of the American Society for Mass Spectrometry, Vol. 20, Issue 12 https://doi.org/10.1016/j.jasms.2009.08.018	journal	December 2009
Ionization and transmission efficiency in an electrospray ionization—mass spectrometry interface Page, Jason S.; Kelly, Ryan T.; Tang, Keqi Journal of the American Society for Mass Spectrometry, Vol. 18, Issue 9, p. 1582-1590 https://doi.org/10.1016/j.jasms.2007.05.018	journal	September 2007
New developments in biochemical mass spectrometry: electrospray ionization Smith, Richard D.; Loo, Joseph A.; Edmonds, Charles G. Analytical Chemistry, Vol. 62, Issue 9 https://doi.org/10.1021/ac00208a002	journal	May 1990
Practical implications of some recent studies in electrospray ionization fundamentals Cech, Nadja B.; Enke, Christie G. Mass Spectrometry Reviews, Vol. 20, Issue 6, p. 362-387 https://doi.org/10.1002/mas.10008	journal	January 2001
Improved Ion Transmission from Atmospheric Pressure to High Vacuum Using a Multicapillary Inlet and Electrodynamic Ion Funnel Interface Kim, Taeman; Udseth, Harold R.; Smith, Richard D. Analytical Chemistry, Vol. 72, Issue 20 https://doi.org/10.1021/ac0003549	journal	October 2000
Improving mass spectrometer sensitivity using a high-pressure electrodynamic ion funnel interface Ibrahim, Yehia; Tang, Keqi; Tolmachev, Aleksey V. Journal of the American Society for Mass Spectrometry, Vol. 17, Issue 9, p. 1299-1305 https://doi.org/10.1016/j.jasms.2006.06.005	journal	September 2006
Ion sampling effects under conditions of total solvent consumption Schneider, Bradley B.; Javaheri, Hassan; Covey, Thomas R. Rapid Communications in Mass Spectrometry, Vol. 20, Issue 10 https://doi.org/10.1002/rcm.2511	journal	January 2006
Inlet Ionization: A New Highly Sensitive Approach for Liquid Chromatography/Mass Spectrometry of Small and Large Molecules Pagnotti, Vincent S.; Inutan, Ellen D.; Marshall, Darrell D. Analytical Chemistry, Vol. 83, Issue 20 https://doi.org/10.1021/ac201982r	journal	October 2011
A New Approach to High Sensitivity Liquid Chromatography-Mass Spectrometry of Peptides using Nanoflow Solvent Assisted Inlet Ionization Wang, Beixi; Inutan, Ellen D.; Trimpin, Sarah Journal of The American Society for Mass Spectrometry, Vol. 23, Issue 3 https://doi.org/10.1007/s13361-011-0320-8	journal	January 2012
Increasing the Sensitivity of Liquid Introduction Mass Spectrometry by Combining Electrospray Ionization and Solvent Assisted Inlet Ionization Pagnotti, Vincent S.; Chakrabarty, Shubhashis; Harron, Andrew F. Analytical Chemistry, Vol. 84, Issue 15 https://doi.org/10.1021/ac3014115	journal	July 2012
Subambient Pressure Ionization with Nanoelectrospray Source and Interface for Improved Sensitivity in Mass Spectrometry Page, Jason S.; Tang, Keqi; Kelly, Ryan T. Analytical Chemistry, Vol. 80, Issue 5 https://doi.org/10.1021/ac702354b	journal	March 2008
Improving Liquid Chromatography-Mass Spectrometry Sensitivity Using a Subambient Pressure Ionization with Nanoelectrospray (SPIN) Interface Tang, Keqi; Page, Jason S.; Marginean, Ioan Journal of The American Society for Mass Spectrometry, Vol. 22, Issue 8 https://doi.org/10.1007/s13361-011-0135-7	journal	April 2011
Improving N -Glycan Coverage using HPLC-MS with Electrospray Ionization at Subambient Pressure Marginean, Ioan; Kronewitter, Scott R.; Moore, Ronald J. Analytical Chemistry, Vol. 84, Issue 21 https://doi.org/10.1021/ac301961u	journal	September 2012
Electrohydrodynamic mass spectrometry Cook, Kelsey D. Mass Spectrometry Reviews, Vol. 5, Issue 4 https://doi.org/10.1002/mas.1280050404	journal	January 1986
On the current emitted by Taylor cone-jets of electrolytes in vacuo: Implications for liquid metal ion sources Gamero-Castaño, M.; Aguirre-de-Carcer, I.; de Juan, L. Journal of Applied Physics, Vol. 83, Issue 5 https://doi.org/10.1063/1.367002	journal	March 1998
Energy distribution and spatial structure of electrosprays of ionic liquids in vacuo Romero-Sanz, I.; Fernandez de la Mora, J. Journal of Applied Physics, Vol. 95, Issue 4 https://doi.org/10.1063/1.1639947	journal	February 2004
Achieving 50% Ionization Efficiency in Subambient Pressure Ionization with Nanoelectrospray Marginean, Ioan; Page, Jason S.; Tolmachev, Aleksey V. Analytical Chemistry, Vol. 82, Issue 22 https://doi.org/10.1021/ac1019123	journal	November 2010
Analytical Properties of the Nanoelectrospray Ion Source Wilm, Matthias; Mann, Matthias Analytical Chemistry, Vol. 68, Issue 1 https://doi.org/10.1021/ac9509519	journal	January 1996
Efficiency of nano-electrospray ionization El-Faramawy, Ayman; Siu, K. W. Michael; Thomson, Bruce A. Journal of the American Society for Mass Spectrometry, Vol. 16, Issue 10 https://doi.org/10.1016/j.jasms.2005.06.011	journal	October 2005
Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS Kelly, Ryan T.; Page, Jason S.; Zhao, Rui Analytical Chemistry, Vol. 80, Issue 1 https://doi.org/10.1021/ac701647s	journal	January 2008
Multinozzle Emitter Arrays for Nanoelectrospray Mass Spectrometry Mao, Pan; Wang, Hung-Ta; Yang, Peidong Analytical Chemistry, Vol. 83, Issue 15, p. 6082-6089 https://doi.org/10.1021/ac2011813	journal	August 2011
Nanoelectrospray emitters: Trends and perspective Gibson, Graham T. T.; Mugo, Samuel M.; Oleschuk, Richard D. Mass Spectrometry Reviews, Vol. 28, Issue 6, p. 918-936 https://doi.org/10.1002/mas.20248	journal	November 2009
Generation of Multiple Electrosprays Using Microfabricated Emitter Arrays for Improved Mass Spectrometric Sensitivity Tang, Keqi; Lin, Yuehe; Matson, Dean W. Analytical Chemistry, Vol. 73, Issue 8 https://doi.org/10.1021/ac001191r	journal	April 2001
Multiple electrosprays generated from a single polycarbonate microstructured fibre: Multiple electrosprays from a polycarbonate MSF Gibson, Graham T. T.; Wright, Ramin D.; Oleschuk, Richard D. Journal of Mass Spectrometry, Vol. 47, Issue 3 https://doi.org/10.1002/jms.2039	journal	March 2012
Array of Chemically Etched Fused-Silica Emitters for Improving the Sensitivity and Quantitation of Electrospray Ionization Mass Spectrometry Kelly, Ryan T.; Page, Jason S.; Tang, Keqi Analytical Chemistry, Vol. 79, Issue 11 https://doi.org/10.1021/ac062417e	journal	June 2007
Nanoelectrospray Emitter Arrays Providing Interemitter Electric Field Uniformity Kelly, Ryan T.; Page, Jason S.; Marginean, Ioan Analytical Chemistry, Vol. 80, Issue 14 https://doi.org/10.1021/ac800508q	journal	July 2008
Microstructured Photonic Fibers as Multichannel Electrospray Emitters Su, Shuqin; Gibson, Graham T. T.; Mugo, Samuel M. Analytical Chemistry, Vol. 81, Issue 17 https://doi.org/10.1021/ac901026t	journal	August 2009
Microfabricated Monolithic Multinozzle Emitters for Nanoelectrospray Mass Spectrometry Kim, Woong; Guo, Mingquan; Yang, Peidong Analytical Chemistry, Vol. 79, Issue 10, p. 3703-3707 https://doi.org/10.1021/ac070010j	journal	May 2007
Simulation and parametric study of a novel multi-spray emitter for ESI–MS applications Sen, A. K.; Darabi, J.; Knapp, D. R. Microfluidics and Nanofluidics, Vol. 3, Issue 3 https://doi.org/10.1007/s10404-006-0122-7	journal	October 2006
Production of protein nanoparticles by electrospray drying Gomez, A.; Bingham, D.; Juan, L. de Journal of Aerosol Science, Vol. 29, Issue 5-6 https://doi.org/10.1016/S0021-8502(97)10031-3	journal	June 1998
Source of heavy molecular ions based on Taylor cones of ionic liquids operating in the pure ion evaporation regime Romero-Sanz, I.; Bocanegra, R.; Fernandez de la Mora, J. Journal of Applied Physics, Vol. 94, Issue 5 https://doi.org/10.1063/1.1598281	journal	September 2003
Influence of space charge on the scale-up of multiplexed electrosprays Deng, Weiwei; Gomez, Alessandro Journal of Aerosol Science, Vol. 38, Issue 10 https://doi.org/10.1016/j.jaerosci.2007.08.005	journal	October 2007
Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801 https://doi.org/10.1021/ac061133r	journal	November 2006

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