On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces

Cox, Jonathan T.; Marginean, Ioan; Smith, Richard D.; Tang, Keqi

doi:10.1007/s13361-014-0998-5

Title: On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces

Journal Article · Tue Sep 30 00:00:00 EDT 2014 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/s13361-014-0998-5· OSTI ID:1166814

Cox, Jonathan T. ^[1]; Marginean, Ioan ^[1]; Smith, Richard D. ^[1]; Tang, Keqi ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division

It is well known that the achievable sensitivity of electrospray ionization mass spectrometry (ESI-MS) is largely determined by the ionization efficiency in the ESI source and ion transmission efficiency through the ESI-MS interface. In this report we systematically study the ion transmission and ionization efficiencies in different ESI-MS interface configurations. The configurations under investigation include a single emitter/single inlet capillary, single emitter/multi-inlet capillary, and a subambient pressure ionization with nanoelectrospray (SPIN) MS interfaces with a single emitter and an emitter array, respectively. We present an effective method to evaluate the overall ion utilization efficiency of an ESI-MS interface by measuring the total gas phase ion current transmitted through the interface and correlating it to the observed ion abundance measured in the corresponding mass spectrum. Our experimental results suggest that the overall ion utilization efficiency in the SPIN-MS interface configurations is better than that in the inlet capillary based ESI-MS interface configurations.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RL01830; AC05-76RLO01830; 1R33CA155252; GM103493-12

OSTI ID:: 1166814

Report Number(s):: PNNL-SA-103142; 47301; 48135; 47418; 400412000

Journal Information:: Journal of the American Society for Mass Spectrometry, Vol. 26, Issue 1; ISSN 1044-0305

Publisher:: American Society for Mass SpectrometryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 15 works

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Title: On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces

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