Electrode assemblies composed of redox cascades from microbial respiratory electron transfer chains

Journal Article · Tue Oct 01 00:00:00 EDT 2013 · Biochemical Society Transactions

DOI:https://doi.org/10.1042/BST20130147· OSTI ID:1158508

Gates, Andrew J. ^[1]; Marritt, Sophie ^[1]; Bradley, Justin ^[1]; Shi, Liang ^[2]; McMillan, Duncan G. ^[3]; Jeuken, Lars J. ^[3]; Richardson, David ^[1]; Butt, Julea N. ^[1]

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1. Univ. of East Anglia, Norwich (United Kingdom)
2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
3. Univ. of Leeds (United Kingdom)

Respiratory and photosynthetic electron transfer chains are dependent on vectorial electron transfer through a series of redox proteins. Examples include electron transfer from NapC to NapAB nitrate reductase in Paracoccus denitrificans and from CymA to Fcc₃ (flavocytochrome c₃) fumarate reductase in Shewanella oneidensis MR-1. In the present article, we demonstrate that graphite electrodes can serve as surfaces for the stepwise adsorption of NapC and NapAB, and the stepwise adsorption of CymA and Fcc₃. Aspects of the catalytic properties of these assemblies are different from those of NapAB and Fcc₃ adsorbed in isolation. We propose that this is due to the formation of NapC-NapAB and of CymA-Fcc₃ complexes that are capable of supporting vectorial electron transfer.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1158508
• Report Number(s): PNNL-SA-98973; KP1702030
• Journal Information: Biochemical Society Transactions, Vol. 41, Issue 5; ISSN 0300-5127
• Publisher: Portland Press
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
electrode assemblies
redox cascades
microbial respiratory
electron transfer chains