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Title: Lipid-Based Nanodiscs as Models for Studying Mesoscale Coalescence A Transport Limited Case

Lipid-based nanodiscs (bicelles) are able to form in mixtures of long- and short-chain lipids. Initially, they are of uniform size but grow upon dilution. Previously, nanodisc growth kinetics have been studied using time-resolved small angle neutron scattering (SANS), a technique which is not well suited for probing their change in size immediately after dilution. To address this, we have used dynamic light scattering (DLS), a technique which permits the collection of useful data in a short span of time after dilution of the system. The DLS data indicate that the negatively charged lipids in nanodiscs play a significant role in disc stability and growth. Specifically, the charged lipids are most likely drawn out from the nanodiscs into solution, thereby reducing interparticle repulsion and enabling the discs to grow. We describe a population balance model, which takes into account Coulombic interactions and adequately predicts the initial growth of nanodiscs with a single parameter i.e., surface potential. The results presented here strongly support the notion that the disc coalescence rate strongly depends on nanoparticle charge density. The present system containing low-polydispersity lipid nanodiscs serves as a good model for understanding how charged discoidal micelles coalesce.
 [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [1]
  1. University of Connecticut, Storrs
  2. ORNL
Publication Date:
OSTI Identifier:
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Soft Matter; Journal Volume: 10; Journal Issue: 28
Research Org:
Oak Ridge National Laboratory (ORNL); High Flux Isotope Reactor (HFIR)
Sponsoring Org:
SC USDOE - Office of Science (SC)
Country of Publication:
United States