Extraction and analysis of neuron firing signals from deep cortical video microscopy

Conference · Wed Jan 01 00:00:00 EST 2014

OSTI ID:1156773

Kerekes, Ryan A ^[1]; Blundon, Jay ^[2]

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1. ORNL
2. St. Jude Children's Research Hospital

We introduce a method for extracting and analyzing neuronal activity time signals from video of the cortex of a live animal. The signals correspond to the firing activity of individual cortical neurons. Activity signals are based on the changing fluorescence of calcium indicators in the cells over time. We propose a cell segmentation method that relies on a user-specified center point, from which the signal extraction method proceeds. A stabilization approach is used to reduce tissue motion in the video. The extracted signal is then processed to flatten the baseline and detect action potentials. We show results from applying the method to a cortical video of a live mouse.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1156773
• Resource Relation: Conference: 2014 Annual ORNL Biomedical Science and Engineering Center Conference, Oak Ridge, TN, USA, 20140506, 20140508
• Country of Publication: United States
• Language: English