Identification of potential molecular markers of ionizing radiation-induced mutations at the hprt locus in CHO cells
- Argonne National Laboratory, IL (United States)
- Univ. of Texas Medical Branch, Galveston, TX (United States); and others
Using multiplex polymerase chain reaction-based exon deletion analysis, we have analyzed mutations at the hprt locus from independent CHO cell mutants isolated from untreated, {sup 60}Co x-ray-, and {sup 212}Bi-exposed CHO-K1 cello and its radiation-sensitive derivative, xrs-5. In the 71 spontaneous CHO-K1 mutants analyzed, 78% showed no change in exon number or size, 20% showed loss of 1-8 exons (partial deletion), and 3% showed loss of all nine hprt exons (total deletion). Exposure of CHO-K1 cells to 6 Gy of {gamma} rays (10% survival) produced 45% of the 20 mutants analyzed showing partial deletion, and 30% showing total deletion. Exposure to an equitoxic dose of a radiation from {sup 212}Bi, a {sup 220}Rn daughter, resulted in a spectrum similar to the {gamma}-ray spectrum in that more than 75% of the 49 mutants analyzed were deletions. The {alpha}-radiation, however, tended to produce larger intragenic deletions that {gamma} radiation. Of the 87 spontaneous xrs-5 mutants analyzed for deletions 44% showed partial deletion, and 14% showed total deletion. Exposure to {alpha} radiation (10% survival) resulted in a deletion spectrum similar to that seen in CHO-K1 cells. Of the 49 mutants analyzed, 43% showed no change in exon number or size, 16% showed partial deletion, and 41% showed total deletion. While the defect in xrs-5 has a profound effect on spontaneous mutation spectra, it does not appear to affect {alpha}-induced mutation spectra.
- OSTI ID:
- 115146
- Report Number(s):
- CONF-9503160-; ISSN 0893-6692; CNN: Grant 1RO1CA56434; TRN: 95:023018
- Journal Information:
- Environmental and Molecular Mutagenesis, Vol. 25, Issue Suppl.25; Conference: 26. annual Environmental Mutagen Society meeting, St. Louis, MO (United States), 12-16 Mar 1995; Other Information: PBD: 1995
- Country of Publication:
- United States
- Language:
- English
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