Diverse and divergent protein post-translational modifications in two growth stages of a natural microbial community

Li, Zhou; Wang, Yingfeng; Yao, Qiuming; Justice, Nicholas B.; Ahn, Tae-Hyuk; Xu, Dong; Banfield, Jillian F.; Pan, Chongle

doi:10.1038/ncomms5405

Title: Diverse and divergent protein post-translational modifications in two growth stages of a natural microbial community

Journal Article · Wed Jan 01 00:00:00 EST 2014 · Nature Communications

DOI:https://doi.org/10.1038/ncomms5405· OSTI ID:1150361

Li, Zhou ^[1]; Wang, Yingfeng ^[1]; Yao, Qiuming ^[2]; Justice, Nicholas B. ^[3]; Ahn, Tae-Hyuk ^[1]; Xu, Dong ^[2]; Banfield, Jillian F. ^[3]; Pan, Chongle ^[1]

ORNL
University of Missouri, Columbia
University of California, Berkeley

Detailed characterization of posttranslational modifications (PTMs) of proteins in microbial communities remains a significant challenge. Here we directly identify and quantify a broad range of PTMs (hydroxylation, methylation, citrullination, acetylation, phosphorylation, methylthiolation, S-nitrosylation and nitration) in a natural microbial community from an acid mine drainage site. Approximately 29% of the identified proteins of the dominant Leptospirillum group II bacteria are modified, and 43% of modified proteins carry multiple PTM types. Most PTM events, except S-nitrosylations, have low fractional occupancy. Notably, PTM events are detected on Cas proteins involved in antiviral defense, an aspect of Cas biochemistry not considered previously. Further, Cas PTM profiles from Leptospirillum group II differ in early versus mature biofilms. PTM patterns are divergent on orthologues of two closely related, but ecologically differentiated, Leptospirillum group II bacteria. Our results highlight the prevalence and dynamics of PTMs of proteins, with potential significance for ecological adaptation and microbial evolution.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: DE-AC05-00OR22725

OSTI ID:: 1150361

Journal Information:: Nature Communications, Journal Name: Nature Communications

Country of Publication:: United States

Language:: English

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