Extracellular secretion of recombinant proteins

Linger, Jeffrey G.; Darzins, Aldis

Title: Extracellular secretion of recombinant proteins

Patent · Tue Jul 22 00:00:00 EDT 2014

OSTI ID:1149687

Linger, Jeffrey G.; Darzins, Aldis

Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.

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Research Organization:: National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC36-08G028308

Assignee:: Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Number(s):: 8,785,159

Application Number:: 12/950,442

OSTI ID:: 1149687

Resource Relation:: Patent File Date: 2010 Nov 19

Country of Publication:: United States

Language:: English

References (20)

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