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Title: Evaluation of High Throughput Screening Methods in Picking up Differences between Cultivars of Lignocellulosic Biomass for Ethanol Production

Here, we present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. We found the best correlation of glucose yields between the Riverside and NREL systems (R2 = 0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2 = 0.4269). The three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.
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  1. Univ. of Copenhagen (Denmark)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. Univ. of California, Riverside, CA (United States)
Publication Date:
OSTI Identifier:
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biomass and Bioenergy; Journal Volume: 66; Journal Issue: July 2014
Research Org:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science
Country of Publication:
United States
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY Chemical and Biosciences; Bioenergy; high throughput screening; lignocellulosic ethanol; wheat straw; genetic selection; microscale pretreatment