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Title: Effect of D2O on growth properties and chemical structure of annual ryegrass (Lolium multiflorum)

In present paper, we report the production and detailed structural analysis of deuterium-enriched rye grass (Lolium multiflorum) for neutron scattering experiments. An efficient method to produce deuterated biomass was developed by designing hydroponic perfusion chambers. In preliminary studies, the partial deuterated rye samples were grown in increasing levels of D2O to study the seed germination and the level of deuterium incorporation as a function of D2O concentration. Solution NMR method indicated 36.9 % deuterium incorporation in 50 % D2O grown annual rye samples and further significant increase in the deuterium incorporation level was observed by germinating the rye seedlings in H2O and growing in 50 % D2O inside the perfusion chambers. Moreover, in an effort to compare the substrate characteristics related to enzymatic hydrolysis on deuterated and protiated version of biomass, annual rye grown in 50 % D2O was selected for detailed biomass characterization studies. The compositional analyses, degree of polymerization and cellulose crystallinity were compared with its protiated control. The cellulose molecular weight indicated slight variation with deuteration; however, hemicellulose molecular weights and cellulose crystallinity remain unaffected with the deuteration. Besides the minor differences in biomass components, the development of deuterated biomass for neutron scattering application is essential tomore » understand the complex biomass conversion processes.« less
 [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [1] ;  [2]
  1. ORNL
  2. Georgia Institute of Technology, Atlanta
Publication Date:
OSTI Identifier:
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Agricultural and Food Chemistry; Journal Volume: 62; Journal Issue: 12
Research Org:
Oak Ridge National Laboratory (ORNL); Center for Structural Molecular Biology (CSMB)
Sponsoring Org:
SC USDOE - Office of Science (SC)
Country of Publication:
United States
Lolium multiflorum. Hydroponics. Cellulose. Crystallinity. 13C CPMAS; deuteration