Transformation of gram positive bacteria by sonoporation

Yang, Yunfeng; Li, Yongchao

Title: Transformation of gram positive bacteria by sonoporation

Patent · Tue Mar 11 00:00:00 EDT 2014

OSTI ID:1126704

Yang, Yunfeng; Li, Yongchao

The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.

View Patent

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-000R22725

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 8,669,085

Application Number:: 12/657,885

OSTI ID:: 1126704

Country of Publication:: United States

Language:: English

References (27)

Rhodococcus bacterium for the production of aryl acylamidase Best, David J.; Vaughan, Peter A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5030571 US Patent Document 5,030,571	patent	July 1991
Sonophoretic drug delivery system Ogden, John E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5656016 US Patent Document 5,656,016	patent	August 1997
Ultrasound enhanced bioprocesses: Cholesterol oxidation by Rhodococcus erythropolis Bar, Raphael Biotechnology and Bioengineering, Vol. 32, Issue 5, p. 655-663 https://doi.org/10.1002/bit.260320510	journal	August 1988
Inactivation of microbes using ultrasound: a review Piyasena, P.; Mohareb, E.; McKellar, R. C. International Journal of Food Microbiology, Vol. 87, Issue 3, p. 207-216 https://doi.org/10.1016/S0168-1605(03)00075-8	journal	November 2003
Synthesis and Applications of Small Molecule Libraries Thompson, Lorin A.; Ellman, Jonathan A. Chemical Reviews, Vol. 96, Issue 1 https://doi.org/10.1021/cr9402081	journal	January 1996
Metabolic flux analysis and pharmaceutical production Boghigian, Brett A.; Seth, Gargi; Kiss, Robert Metabolic Engineering, Vol. 12, Issue 2, p. 81-95 https://doi.org/10.1016/j.ymben.2009.10.004	journal	March 2010
Cell-linked and extracellular cholesterol oxidase activities from Rhodococcus erythropolis. Isolation and physiological characterization Sojo, M.; Bru, R.; Lopez-Molina, D. Applied Microbiology and Biotechnology, Vol. 47, Issue 5 https://doi.org/10.1007/s002530050977	journal	May 1997
The polymer physics and chemistry of microbial cell attachment and adhesion Geoghegan, Mark; Andrews, Johanna S.; Biggs, Catherine A. Faraday Discussions, Vol. 139 https://doi.org/10.1039/B717046G	journal	January 2008
Application of electroporation for transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus, Bacillus, Staphylococcus, Enterococcus and Propionibacterium Luchansky, J. B.; Muriana, P. M.; Klaenhammer, T. R. Molecular Microbiology, Vol. 2, Issue 5, p. 637-646 https://doi.org/10.1111/j.1365-2958.1988.tb00072.x	journal	September 1988
Gene transfer to Clostridium cellulolyticum ATCC 35319 Jennert, Katrin C. B.; Young, Danielle I.; Young, Michael Microbiology, Vol. 146, Issue 12 https://doi.org/10.1099/00221287-146-12-3071	journal	December 2000
Ultrasound: Mechanical gene transfer into plant cells by sonoporation Liu, Y.; Yang, H.; Sakanishi, A. Biotechnology Advances, Vol. 24, Issue 1, p. 1-16 https://doi.org/10.1016/j.biotechadv.2005.04.002	journal	January 2006
Sonophoresis. II. Examination of the Mechanism(s) of Ultrasound-Enhanced Transdermal Drug Delivery Bommannan, D.; Menon, Gopinathan K.; Okuyama, Hirohisa Pharmaceutical Research, Vol. 09, Issue 8, p. 1043-1047 https://doi.org/10.1023/A:1015806528336	journal	January 1992
Ultrasound Enhances Reporter Gene Expression After Transfection of Vascular Cells In Vitro Lawrie, Allan; Brisken, Axel F.; Francis, Sheila E. Circulation, Vol. 99, Issue 20 https://doi.org/10.1161/01.CIR.99.20.2617	journal	May 1999
Gene therapy progress and prospects: Ultrasound for gene transfer Newman, C. M. H.; Bettinger, T. Gene Therapy, Vol. 14, Issue 6 https://doi.org/10.1038/sj.gt.3302925	journal	March 2007
Direct gene transfer to plant protoplasts by mild sonication Joersbo, Morten; Brunstedt, Janne Plant Cell Reports, Vol. 9, Issue 4 https://doi.org/10.1007/BF00232181	journal	August 1990
The Use of Sonication for the Efficient Delivery of Plasmid DNA into Cells Wyber, Julie Ann; Andrews, Julie; D'Emanuele, Antony Pharmaceutical Research, Vol. 14, Issue 6, p. 750-756 https://doi.org/10.1023/A:1012198321879	journal	January 1997
Ultrasound-mediated DNA transfer for bacteria Song, Y.; Hahn, T.; Thompson, I. P. Nucleic Acids Research, Vol. 35, Issue 19 https://doi.org/10.1093/nar/gkm710	journal	October 2007
Sonoporation Is an Efficient Tool for Intracellular Fluorescent Dextran Delivery and One-Step Double-Crossover Mutant Construction in Fusobacterium nucleatum Han, Y. W.; Ikegami, A.; Chung, P. Applied and Environmental Microbiology, Vol. 73, Issue 11 https://doi.org/10.1128/AEM.00428-07	journal	April 2007
Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria Han, Y. W.; Ikegami, A.; Rajanna, C. Journal of Bacteriology, Vol. 187, Issue 15 https://doi.org/10.1128/JB.187.15.5330-5340.2005	journal	July 2005
Engineering microbial consortia: a new frontier in synthetic biology Brenner, Katie; You, Lingchong; Arnold, Frances H. Trends in Biotechnology, Vol. 26, Issue 9, p. 483-489 https://doi.org/10.1016/j.tibtech.2008.05.004	journal	September 2008
Plasma membrane poration induced by ultrasound exposure: Implication for drug delivery Mehier-Humbert, Sophie; Bettinger, Thierry; Yan, Feng Journal of Controlled Release, Vol. 104, Issue 1, p. 213-222 https://doi.org/10.1016/j.jconrel.2005.01.007	journal	May 2005
Mechanism of intracellular delivery by acoustic cavitation Schlicher, Robyn K.; Radhakrishna, Harish; Tolentino, Timothy P. Ultrasound in Medicine & Biology, Vol. 32, Issue 6, p. 915-924 https://doi.org/10.1016/j.ultrasmedbio.2006.02.1416	journal	June 2006
Sequence-specific DNA binding by the Msp l DNA methyltransferase Dubey, Ashok K.; Roberts, Richard J. Nucleic Acids Research, Vol. 20, Issue 12 https://doi.org/10.1093/nar/20.12.3167	journal	January 1992
Design of Antisense RNA Constructs for Downregulation of the Acetone Formation Pathway of Clostridium acetobutylicum Tummala, S. B.; Welker, N. E.; Papoutsakis, E. T. Journal of Bacteriology, Vol. 185, Issue 6 https://doi.org/10.1128/JB.185.6.1923-1934.2003	journal	March 2003
Electrotransformation of Clostridium thermocellum Tyurin, M. V.; Desai, S. G.; Lynd, L. R. Applied and Environmental Microbiology, Vol. 70, Issue 2 https://doi.org/10.1128/AEM.70.2.883-890.2004	journal	February 2004
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002
Role of Spontaneous Current Oscillations during High-Efficiency Electrotransformation of Thermophilic Anaerobes Tyurin, M. V.; Sullivan, C. R.; Lynd, L. R. Applied and Environmental Microbiology, Vol. 71, Issue 12 https://doi.org/10.1128/AEM.71.12.8069-8076.2005	journal	December 2005

Similar Records

Recognition of U-rich RNA by Hfq from the Gram-positive pathogen Listeria monocytogenes

Journal Article · Fri Aug 22 00:00:00 EDT 2014 · RNA · OSTI ID:1126704

Kovach, Alexander R.; Hoff, Kirsten E.; Canty, John T.; +2 more

Methods for targetted mutagenesis in gram-positive bacteria

Patent · Tue May 27 00:00:00 EDT 2014 · OSTI ID:1126704

Yang, Yunfeng

Comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. [Salmonella typhimurium; Escherichia coli; Sarcina lutea; Staphylococcus aureus; Streptococcus lactis; Streptococcus faecalis]

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Journal of Bacteriology; (USA) · OSTI ID:1126704

Dahl, T A; Midden, W R; Hartman, P E

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Transformation of gram positive bacteria by sonoporation

Citation Formats

References (27)

Similar Records

Related Subjects