Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 Å resolution
Here, a 1.82 Å resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an Rfree of 0.196. Structural analysis shows that five related structures have been reported with a secondary-structure similarity of between 75 and 89%. The five similar structures were all either Clostridium thermocellum or Geotrichum sp. M128 GH74 xyloglucanases. Structural analysis indicates that the A. cellulolyticus GH74 enzyme is an endoxyloglucanase, as it lacks a characteristic loop that blocks one end of the active site in exoxyloglucanases. Superimposition with the C. thermocellum GH74 shows that Asp451 and Asp38 are the catalytic residues.
- Research Organization:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1122295
- Report Number(s):
- NREL/JA-2700-60609; ACSFCL
- Journal Information:
- Acta Crystallographica. Section F, Vol. 69, Issue 12; Related Information: Article No. F69; ISSN 1744-3091
- Publisher:
- International Union of Crystallography
- Country of Publication:
- United States
- Language:
- English
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