skip to main content

SciTech ConnectSciTech Connect

Title: Global SAXS Data Analysis for Multilamellar Vesicles: Evolution of the Scattering Density Profile (SDP) Model

The highly successful scattering density profile (SDP) model, used to jointly analyze small-angle X-ray and neutron scattering data from unilamellar vesicles, has been adapted for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). Using a genetic algorithm, this new method is capable of providing high-resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone X-ray data. Structural parameters such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. The results are in good agreement with previously reported SDP data, which used both neutron and X-ray data. The inclusion of deuterated and non-deuterated MLV neutron data in the analysis improved the lipid backbone information but did not improve, within experimental error, the structural data regarding bilayer thickness and area per lipid.
 [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [4] ;  [5] ;  [2] ;  [1]
  1. University of Graz, Institute of Molecular Biosciences, Austria
  2. ORNL
  3. University of Leeds, UK
  4. Graz University of Technology
  5. Atomic Energy of Canada Limited (AECL), Canadian Neutron Beam Centre (CNBC) and Comenius University,
Publication Date:
OSTI Identifier:
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Applied Crystallography; Journal Volume: 47; Journal Issue: 1
Research Org:
Oak Ridge National Laboratory (ORNL); Spallation Neutron Source
Sponsoring Org:
SC USDOE - Office of Science (SC)
Country of Publication:
United States